MED8_SCHPO
ID MED8_SCHPO Reviewed; 200 AA.
AC O94646;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 137.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE AltName: Full=Cell separation protein sep15;
DE AltName: Full=Mediator complex subunit 8;
GN Name=med8; Synonyms=sep15; ORFNames=SPBC21.04;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11191205; DOI=10.1007/s002940000158;
RA Zilahi E., Miklos I., Sipiczki M.;
RT "The Schizosaccharomyces pombe sep15+ gene encodes a protein homologous to
RT the Med8 subunit of the Saccharomyces cerevisiae transcriptional mediator
RT complex.";
RL Curr. Genet. 38:227-232(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RX PubMed=11572939; DOI=10.1073/pnas.211253898;
RA Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D.,
RA Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.;
RT "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential
RT subunits conserved between yeast and metazoan cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:11572939}.
CC -!- INTERACTION:
CC O94646; O74825: rpb4; NbExp=3; IntAct=EBI-1533413, EBI-697320;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC {ECO:0000305}.
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DR EMBL; AF093755; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CU329671; CAB36884.1; -; Genomic_DNA.
DR PIR; T39886; T39886.
DR RefSeq; NP_596338.1; NM_001022259.2.
DR PDB; 3C0T; X-ray; 2.40 A; B=180-200.
DR PDB; 4H63; X-ray; 3.40 A; H=1-200.
DR PDB; 5N9J; X-ray; 3.40 A; U=1-200.
DR PDB; 5U0P; EM; 4.40 A; H=1-200.
DR PDB; 5U0S; EM; 7.80 A; H=1-200.
DR PDBsum; 3C0T; -.
DR PDBsum; 4H63; -.
DR PDBsum; 5N9J; -.
DR PDBsum; 5U0P; -.
DR PDBsum; 5U0S; -.
DR AlphaFoldDB; O94646; -.
DR SMR; O94646; -.
DR BioGRID; 277281; 15.
DR DIP; DIP-38755N; -.
DR IntAct; O94646; 5.
DR MINT; O94646; -.
DR STRING; 4896.SPBC21.04.1; -.
DR MaxQB; O94646; -.
DR PaxDb; O94646; -.
DR EnsemblFungi; SPBC21.04.1; SPBC21.04.1:pep; SPBC21.04.
DR GeneID; 2540761; -.
DR KEGG; spo:SPBC21.04; -.
DR PomBase; SPBC21.04; med8.
DR VEuPathDB; FungiDB:SPBC21.04; -.
DR eggNOG; ENOG502S8U1; Eukaryota.
DR HOGENOM; CLU_108151_0_0_1; -.
DR InParanoid; O94646; -.
DR OMA; PQWYSLQ; -.
DR PhylomeDB; O94646; -.
DR EvolutionaryTrace; O94646; -.
DR PRO; PR:O94646; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0016592; C:mediator complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:PomBase.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:PomBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR019364; Mediatior_Med8_fun/met.
DR PANTHER; PTHR13074; PTHR13074; 1.
DR Pfam; PF10232; Med8; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..200
FT /note="Mediator of RNA polymerase II transcription subunit
FT 8"
FT /id="PRO_0000096396"
FT HELIX 4..37
FT /evidence="ECO:0007829|PDB:4H63"
FT HELIX 43..66
FT /evidence="ECO:0007829|PDB:4H63"
FT HELIX 68..72
FT /evidence="ECO:0007829|PDB:4H63"
FT STRAND 74..78
FT /evidence="ECO:0007829|PDB:4H63"
FT TURN 84..86
FT /evidence="ECO:0007829|PDB:4H63"
FT HELIX 88..94
FT /evidence="ECO:0007829|PDB:4H63"
FT HELIX 101..117
FT /evidence="ECO:0007829|PDB:4H63"
FT HELIX 130..147
FT /evidence="ECO:0007829|PDB:4H63"
FT HELIX 148..150
FT /evidence="ECO:0007829|PDB:4H63"
FT STRAND 154..157
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 160..165
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 184..197
FT /evidence="ECO:0007829|PDB:3C0T"
SQ SEQUENCE 200 AA; 23333 MW; A61A9E49B83A86F0 CRC64;
MEDISTEKTV ESLEAIRHRI AQIVQSLTHF LAILHQSESL SPWPTIHKNF NILLSQIHSL
SNNLAAHSHT LQTTSIYPSL EFPVKEQEPL LTTLLRTKAL PEVEEWEANT LQEYEASISS
QPKKKEANDA YQKDQLWDQA RIIFMEEREN YSWFDFVTRR QESEGEFVSQ RQLEIDRATE
EQNANQMLTD ILSFMKSGKR
