ID   MED8_XENTR              Reviewed;         267 AA.
AC   Q08BU1;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE   AltName: Full=Mediator complex subunit 8;
GN   Name=med8;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors. May play a role as a target
CC       recruitment subunit in E3 ubiquitin-protein ligase complexes and thus
CC       in ubiquitination and subsequent proteasomal degradation of target
CC       proteins (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Component of the Mediator complex. May be part of a
CC       multisubunit E3 ubiquitin-protein ligase complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC       {ECO:0000305}.
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DR   EMBL; BC124562; AAI24563.1; -; mRNA.
DR   RefSeq; NP_001016614.1; NM_001016614.3.
DR   AlphaFoldDB; Q08BU1; -.
DR   SMR; Q08BU1; -.
DR   STRING; 8364.ENSXETP00000021128; -.
DR   PaxDb; Q08BU1; -.
DR   DNASU; 549368; -.
DR   Ensembl; ENSXETT00000083719; ENSXETP00000075936; ENSXETG00000038548.
DR   GeneID; 549368; -.
DR   KEGG; xtr:549368; -.
DR   CTD; 112950; -.
DR   Xenbase; XB-GENE-963498; med8.
DR   eggNOG; KOG3583; Eukaryota.
DR   HOGENOM; CLU_085476_0_0_1; -.
DR   InParanoid; Q08BU1; -.
DR   OMA; IIMRVND; -.
DR   OrthoDB; 1392867at2759; -.
DR   PhylomeDB; Q08BU1; -.
DR   TreeFam; TF316778; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000008143; Chromosome 4.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000038548; Expressed in egg cell and 12 other tissues.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019364; Mediatior_Med8_fun/met.
DR   PANTHER; PTHR13074; PTHR13074; 1.
DR   Pfam; PF10232; Med8; 1.
PE   2: Evidence at transcript level;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Ubl conjugation pathway.
FT   CHAIN           1..267
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   8"
FT                   /id="PRO_0000304528"
FT   REGION          190..267
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          1..26
FT                   /evidence="ECO:0000255"
FT   COILED          116..160
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        205..267
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   267 AA;  29448 MW;  70C43A89E1279B26 CRC64;
     MQREEKQLEA CLDALISQVS DIKNSLVGFI HKLENEYDRL TWPSVLDSFA LLSGQLNTLN
     KVLKNEKTPL LRNQVIIPLL LSPDRDEEIM RLTEGRVPVF SHEVVPDHLR TKPDPDVEEL
     EKQLSAEAAR ITSEAAQKQV QSMNKMCSNL LDKISKEERE SELGSLRQNK QTFNPTDTNA
     LVAAVAFGKG LSNRRPPGQG GPMAPGQTGA SSMLPNATGM QVPMSLPPNQ QQQHMAGVSM
     SQGSQPGKMP SSIKTNIKSA SMHPYQR