ID   MED8_YARLI              Reviewed;         194 AA.
AC   Q6CG02;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE   AltName: Full=Mediator complex subunit 8;
GN   Name=MED8; OrderedLocusNames=YALI0B02068g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC       {ECO:0000305}.
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DR   EMBL; CR382128; CAG82628.1; -; Genomic_DNA.
DR   RefSeq; XP_500410.1; XM_500410.1.
DR   AlphaFoldDB; Q6CG02; -.
DR   SMR; Q6CG02; -.
DR   STRING; 4952.CAG82628; -.
DR   EnsemblFungi; CAG82628; CAG82628; YALI0_B02068g.
DR   GeneID; 2907557; -.
DR   KEGG; yli:YALI0B02068g; -.
DR   VEuPathDB; FungiDB:YALI0_B02068g; -.
DR   HOGENOM; CLU_108151_0_0_1; -.
DR   InParanoid; Q6CG02; -.
DR   OMA; PQWYSLQ; -.
DR   Proteomes; UP000001300; Chromosome B.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019364; Mediatior_Med8_fun/met.
DR   PANTHER; PTHR13074; PTHR13074; 1.
DR   Pfam; PF10232; Med8; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..194
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   8"
FT                   /id="PRO_0000304547"
SQ   SEQUENCE   194 AA;  21744 MW;  A673F3F5B219647B CRC64;
     MNQPIEPDHI NVPTEALESL RLRLTQVSHS LNTLQAQLHQ PTLPPWSSLH NQFNVLLTQL
     VSLSSTITHQ SDILQQTVTF PLPAFPTATE AGLMATLLRK KILPEVEEWC EEVKQKALGV
     KIRTVDQYGE WAAETVEEAK QEYEWYGLMT REEVDNGVKP PVYVAPEEEA GEGAKLTIEQ
     ILQFTCAGKM PTVA