MED9_ARATH
ID MED9_ARATH Reviewed; 244 AA.
AC Q8RWA2; Q9C724;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 9;
GN Name=MED9; Synonyms=MED9_1; OrderedLocusNames=At1g55080; ORFNames=T7N22.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [6]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [7]
RP INTERACTION WITH ME14/CBP1.
RX PubMed=26462908; DOI=10.1105/tpc.15.00370;
RA Li H.J., Zhu S.S., Zhang M.X., Wang T., Liang L., Xue Y., Shi D.Q., Liu J.,
RA Yang W.C.;
RT "Arabidopsis CBP1 is a novel regulator of transcription initiation in
RT central cell-mediated pollen tube guidance.";
RL Plant Cell 27:2880-2893(2015).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional pre-initiation complex with RNA polymerase II and the
CC general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex (PubMed:17560376). Interacts
CC with MEE14/CBP1 (PubMed:26462908). {ECO:0000269|PubMed:17560376,
CC ECO:0000269|PubMed:26462908}.
CC -!- INTERACTION:
CC Q8RWA2; Q9LZ00: MED4; NbExp=5; IntAct=EBI-1386224, EBI-1386214;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the plant Mediator complex subunit 9 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50844.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC073944; AAG50844.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33183.1; -; Genomic_DNA.
DR EMBL; AY093241; AAM13240.1; -; mRNA.
DR EMBL; BT006281; AAP13389.1; -; mRNA.
DR EMBL; AK227122; BAE99172.1; -; mRNA.
DR PIR; D96592; D96592.
DR RefSeq; NP_175905.2; NM_104382.5.
DR AlphaFoldDB; Q8RWA2; -.
DR SMR; Q8RWA2; -.
DR IntAct; Q8RWA2; 4.
DR STRING; 3702.AT1G55080.1; -.
DR PaxDb; Q8RWA2; -.
DR PRIDE; Q8RWA2; -.
DR ProteomicsDB; 228828; -.
DR EnsemblPlants; AT1G55080.1; AT1G55080.1; AT1G55080.
DR GeneID; 841951; -.
DR Gramene; AT1G55080.1; AT1G55080.1; AT1G55080.
DR KEGG; ath:AT1G55080; -.
DR Araport; AT1G55080; -.
DR TAIR; locus:2205662; AT1G55080.
DR eggNOG; ENOG502RYJY; Eukaryota.
DR HOGENOM; CLU_120190_0_0_1; -.
DR InParanoid; Q8RWA2; -.
DR OMA; ANYRKSV; -.
DR OrthoDB; 1535477at2759; -.
DR PRO; PR:Q8RWA2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8RWA2; baseline and differential.
DR Genevisible; Q8RWA2; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR InterPro; IPR038790; Med9_plant.
DR PANTHER; PTHR37188; PTHR37188; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..244
FT /note="Mediator of RNA polymerase II transcription subunit
FT 9"
FT /id="PRO_0000419197"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 96..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 212..239
FT /evidence="ECO:0000255"
SQ SEQUENCE 244 AA; 28585 MW; BF795E4ED4047FEE CRC64;
MDQFSGGGGN WSMIPNVQAQ GNFGTPTNHD QQLFLQQQQL QQQQQQQQQQ QFHLQQQQQT
QQQQQQFQPQ QQQEMQQYQQ FQQQQHFIQQ QQFQQQQRLL QSPPLQPQSL QSPPPQQTMV
HTPQSMMHTP QQQQQLVQTP VQTPQQHQSL ASHFHLYPMV EKLADVIENG TRDQNSDALV
NELNSHFDKC QQLLNSISGS LGSKTMTVDG QKRNVEESEQ LLQQRRDLIV EYRKSIEEIV
TMEH
