MEDAG_HUMAN
ID MEDAG_HUMAN Reviewed; 303 AA.
AC Q5VYS4; Q8IXF1; Q96K26; Q96NC8;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Mesenteric estrogen-dependent adipogenesis protein;
DE AltName: Full=Activated in W/Wv mouse stomach 3 homolog;
DE Short=hAWMS3;
DE AltName: Full=Mesenteric estrogen-dependent adipose 4;
DE Short=MEDA-4;
GN Name=MEDAG; Synonyms=AWMS3, C13orf33, MEDA4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP GLY-59.
RC TISSUE=Ovary, and Synovial cell;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057823; DOI=10.1038/nature02379;
RA Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S.,
RA Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Andrews D.T., Ashwell R.I.S., Babbage A.K., Bagguley C.L.,
RA Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P.,
RA Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C.,
RA Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P.,
RA Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L.,
RA Frankish A.G., Frankland J., French L., Garner P., Garnett J.,
RA Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M.,
RA Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D.,
RA Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D.,
RA Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S.,
RA Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R.,
RA Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W.,
RA Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P.,
RA Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L.,
RA Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R.,
RA Rogers J., Ross M.T.;
RT "The DNA sequence and analysis of human chromosome 13.";
RL Nature 428:522-528(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT GLY-59.
RC TISSUE=Heart, Lung, and Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 92-303 (ISOFORM 1).
RA Daigo Y., Takayama I., Fujino M.A.;
RT "Isolation and characterization of novel human and mouse genes, which are
RT expressed in the digestive tract.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=22510272; DOI=10.1210/en.2011-2008;
RA Zhang H., Chen X., Sairam M.R.;
RT "Novel genes of visceral adiposity: identification of mouse and human
RT mesenteric estrogen-dependent adipose (MEDA)-4 gene and its adipogenic
RT function.";
RL Endocrinology 153:2665-2676(2012).
CC -!- FUNCTION: Involved in processes that promote adipocyte differentiation,
CC lipid accumulation, and glucose uptake in mature adipocytes.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q5VYS4; Q8N5M1: ATPAF2; NbExp=3; IntAct=EBI-1050881, EBI-1166928;
CC Q5VYS4; Q7Z7H3: CATIP; NbExp=3; IntAct=EBI-1050881, EBI-10258233;
CC Q5VYS4; P57105: SYNJ2BP; NbExp=3; IntAct=EBI-1050881, EBI-1049004;
CC Q5VYS4; Q9H0E2: TOLLIP; NbExp=3; IntAct=EBI-1050881, EBI-74615;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22510272}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5VYS4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5VYS4-2; Sequence=VSP_022714, VSP_022715;
CC -!- TISSUE SPECIFICITY: Highly expressed in the visceral fat depot.
CC {ECO:0000269|PubMed:22510272}.
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DR EMBL; AK027740; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK055635; BAB70975.1; -; mRNA.
DR EMBL; AL353680; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC040165; AAH40165.1; -; mRNA.
DR EMBL; BC101624; AAI01625.1; -; mRNA.
DR EMBL; BC101626; AAI01627.1; -; mRNA.
DR EMBL; AB055407; BAC53805.1; -; mRNA.
DR CCDS; CCDS9338.1; -. [Q5VYS4-1]
DR RefSeq; NP_116238.2; NM_032849.3. [Q5VYS4-1]
DR AlphaFoldDB; Q5VYS4; -.
DR BioGRID; 124369; 5.
DR IntAct; Q5VYS4; 5.
DR STRING; 9606.ENSP00000369849; -.
DR iPTMnet; Q5VYS4; -.
DR PhosphoSitePlus; Q5VYS4; -.
DR BioMuta; MEDAG; -.
DR MassIVE; Q5VYS4; -.
DR PaxDb; Q5VYS4; -.
DR PeptideAtlas; Q5VYS4; -.
DR PRIDE; Q5VYS4; -.
DR ProteomicsDB; 65640; -. [Q5VYS4-1]
DR ProteomicsDB; 65641; -. [Q5VYS4-2]
DR Antibodypedia; 53455; 18 antibodies from 9 providers.
DR DNASU; 84935; -.
DR Ensembl; ENST00000380482.9; ENSP00000369849.4; ENSG00000102802.10. [Q5VYS4-1]
DR GeneID; 84935; -.
DR KEGG; hsa:84935; -.
DR MANE-Select; ENST00000380482.9; ENSP00000369849.4; NM_032849.4; NP_116238.3.
DR UCSC; uc001uth.5; human. [Q5VYS4-1]
DR CTD; 84935; -.
DR DisGeNET; 84935; -.
DR GeneCards; MEDAG; -.
DR HGNC; HGNC:25926; MEDAG.
DR HPA; ENSG00000102802; Tissue enhanced (adipose).
DR neXtProt; NX_Q5VYS4; -.
DR OpenTargets; ENSG00000102802; -.
DR PharmGKB; PA147358556; -.
DR VEuPathDB; HostDB:ENSG00000102802; -.
DR eggNOG; ENOG502REG3; Eukaryota.
DR GeneTree; ENSGT00390000018451; -.
DR HOGENOM; CLU_080194_0_0_1; -.
DR InParanoid; Q5VYS4; -.
DR OMA; PGNEVIN; -.
DR OrthoDB; 1192582at2759; -.
DR PhylomeDB; Q5VYS4; -.
DR TreeFam; TF328824; -.
DR PathwayCommons; Q5VYS4; -.
DR SignaLink; Q5VYS4; -.
DR BioGRID-ORCS; 84935; 13 hits in 1065 CRISPR screens.
DR ChiTaRS; MEDAG; human.
DR GenomeRNAi; 84935; -.
DR Pharos; Q5VYS4; Tdark.
DR PRO; PR:Q5VYS4; -.
DR Proteomes; UP000005640; Chromosome 13.
DR RNAct; Q5VYS4; protein.
DR Bgee; ENSG00000102802; Expressed in calcaneal tendon and 149 other tissues.
DR ExpressionAtlas; Q5VYS4; baseline and differential.
DR Genevisible; Q5VYS4; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0045600; P:positive regulation of fat cell differentiation; ISS:UniProtKB.
DR InterPro; IPR039230; MEDAG.
DR InterPro; IPR043460; MEDAG/TEX26.
DR PANTHER; PTHR33769; PTHR33769; 1.
DR PANTHER; PTHR33769:SF3; PTHR33769:SF3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Reference proteome.
FT CHAIN 1..303
FT /note="Mesenteric estrogen-dependent adipogenesis protein"
FT /id="PRO_0000274333"
FT VAR_SEQ 1..114
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_022714"
FT VAR_SEQ 223..262
FT /note="NLSSTSPEKKETIKLFLEKMSEPLIRRSSFSDRKFSVTSR -> L (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_022715"
FT VARIANT 59
FT /note="R -> G (in dbSNP:rs9531945)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_030261"
SQ SEQUENCE 303 AA; 34190 MW; AAE14F897EA2C484 CRC64;
MAGAACEPVA RPSLTSISSG ELRSLWTCDC ELALLPLAQL LRLQPGAFQL SGDQLVVARP
GEPAAARGGF NVFGDGLVRL DGQLYRLSSY IKRYVELTNY CDYKDYRETI LSKPMLFFIN
VQTKKDTSKE RTYAFLVNTR HPKIRRQIEQ GMDMVISSVI GESYRLQFDF QEAVKNFFPP
GNEVVNGENL SFAYEFKADA LFDFFYWFGL SNSVVKVNGK VLNLSSTSPE KKETIKLFLE
KMSEPLIRRS SFSDRKFSVT SRGSIDDVFN CNLSPRSSLT EPLLAELPFP SVLESEETPN
QFI
