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MEFV_RAT
ID   MEFV_RAT                Reviewed;         747 AA.
AC   Q9JJ25; A0A0G2JYG3;
DT   19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-FEB-2022, sequence version 2.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Pyrin {ECO:0000303|PubMed:10818206};
GN   Name=Mefv {ECO:0000303|PubMed:10818206, ECO:0000312|RGD:61889};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=Sprague-Dawley;
RX   PubMed=10818206; DOI=10.1007/s003350010082;
RA   Chae J.J., Centola M., Aksentijevich I., Dutra A., Tran M., Wood G.,
RA   Nagaraju K., Kingma D.W., Liu P.P., Kastner D.L.;
RT   "Isolation, genomic organization, and expression analysis of the mouse and
RT   rat homologs of MEFV, the gene for familial Mediterranean fever.";
RL   Mamm. Genome 11:428-435(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RX   PubMed=15057822; DOI=10.1038/nature02426;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA   Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA   Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA   Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA   Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA   Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA   Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA   Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA   Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA   Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA   Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA   Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA   Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA   Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA   Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA   Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA   Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA   Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA   Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA   Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA   Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA   Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA   Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA   Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA   Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA   Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA   Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA   Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA   Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA   Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA   Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA   Mockrin S., Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into mammalian
RT   evolution.";
RL   Nature 428:493-521(2004).
CC   -!- FUNCTION: Involved in the regulation of innate immunity and the
CC       inflammatory response in response to IFNG/IFN-gamma. Organizes
CC       autophagic machinery by serving as a platform for the assembly of ULK1,
CC       Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes
CC       specific autophagy targets, thus coordinating target recognition with
CC       assembly of the autophagic apparatus and initiation of autophagy. Acts
CC       as an autophagy receptor for the degradation of several inflammasome
CC       components, including CASP1, NLRP1 and NLRP3, hence preventing
CC       excessive IL1B- and IL18-mediated inflammation. However, it can also
CC       have a positive effect in the inflammatory pathway, acting as an innate
CC       immune sensor that triggers PYCARD/ASC specks formation, caspase-1
CC       activation, and IL1B and IL18 production (By similarity). Together with
CC       AIM2, also acts as a mediator of pyroptosis, necroptosis and apoptosis
CC       (PANoptosis), an integral part of host defense against pathogens, in
CC       response to bacterial infection (By similarity). It is required for
CC       PSTPIP1-induced PYCARD/ASC oligomerization and inflammasome formation.
CC       Recruits PSTPIP1 to inflammasomes, and is required for PSTPIP1
CC       oligomerization (By similarity). {ECO:0000250|UniProtKB:O15553,
CC       ECO:0000250|UniProtKB:Q9JJ26}.
CC   -!- SUBUNIT: Homotrimer. Interacts (via the B box-type zinc finger) with
CC       PSTPIP1. Interacts (via the B30.2/SPRY domain) with several components
CC       of the inflammasome complex, including CASP1 p20 and p10 subunits,
CC       CASP5, PYCARD, NLRP1, NLRP2 AND NLRP3, as well as with unprocessed
CC       IL1B; this interaction may lead to autophagic degradation of these
CC       proteins (By similarity). Component of the AIM2 PANoptosome complex, a
CC       multiprotein complex that drives inflammatory cell death (PANoptosis)
CC       (By similarity). Interacts with NFKBIA and RELA. Interacts weakly with
CC       VASP and ACTR3. Interacts with active ULK1 (phosphorylated on 'Ser-
CC       317') and BECN1 simultaneously. Also interacts with ATG16L1 (via WD
CC       repeats), and with ATG8 family members, including GABARAP, GABARAPL1
CC       and, to a lesser extent, GABARAPL2, MAP1LC3A/LC3A and MAP1LC3C/LC3C.
CC       Interacts with TRIM21. Interacts with YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ
CC       AND YWHAZ; the interaction is required for the down-regulation of pyrin
CC       pro-inflammatory activity (By similarity).
CC       {ECO:0000250|UniProtKB:O15553, ECO:0000250|UniProtKB:Q9JJ26}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:O15553}. Cell projection, ruffle
CC       {ECO:0000250|UniProtKB:O15553}. Cell projection, lamellipodium
CC       {ECO:0000250|UniProtKB:O15553}. Cytoplasm
CC       {ECO:0000250|UniProtKB:O15553}. Cytoplasmic vesicle, autophagosome
CC       {ECO:0000250|UniProtKB:O15553}. Nucleus {ECO:0000250|UniProtKB:O15553}.
CC       Note=Associated with microtubules and with the filamentous actin of
CC       perinuclear filaments and peripheral lamellar ruffles. In pre-apoptotic
CC       cells, colocalizes with PYCARD/ASC in large specks (pyroptosomes). In
CC       migrating monocytes, strongly polarized at the leading edge of the cell
CC       where it colocalizes with polymerizing actin and PYCARD/ASC (By
CC       similarity). {ECO:0000250|UniProtKB:O15553}.
CC   -!- TISSUE SPECIFICITY: Expressed in spleen and, to a lesser degree in the
CC       lung. Not expressed in thymus, testis, ovary, heart, brain, liver,
CC       kidney and muscle. {ECO:0000269|PubMed:10818206}.
CC   -!- DOMAIN: The B box-type zinc finger interacts, possibly
CC       intramolecularly, with the pyrin domain; this may be an autoinhibitory
CC       mechanism released by PSTPIP1 binding. {ECO:0000250|UniProtKB:O15553}.
CC   -!- PTM: Degraded along with the delivery of its substrates to
CC       autolysosomal compartments (at protein level).
CC       {ECO:0000250|UniProtKB:O15553}.
CC   -!- CAUTION: Lacks the B30.2/SPRY domain found in the human ortholog, thus
CC       may have divergent function(s). {ECO:0000305}.
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DR   EMBL; AF143410; AAF03767.1; -; mRNA.
DR   EMBL; AABR07072080; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_113822.1; NM_031634.1.
DR   AlphaFoldDB; Q9JJ25; -.
DR   SMR; Q9JJ25; -.
DR   STRING; 10116.ENSRNOP00000011073; -.
DR   iPTMnet; Q9JJ25; -.
DR   PhosphoSitePlus; Q9JJ25; -.
DR   PaxDb; Q9JJ25; -.
DR   PRIDE; Q9JJ25; -.
DR   GeneID; 58923; -.
DR   KEGG; rno:58923; -.
DR   UCSC; RGD:61889; rat.
DR   CTD; 4210; -.
DR   RGD; 61889; Mefv.
DR   eggNOG; KOG2177; Eukaryota.
DR   InParanoid; Q9JJ25; -.
DR   OrthoDB; 423686at2759; -.
DR   PhylomeDB; Q9JJ25; -.
DR   Reactome; R-RNO-844456; The NLRP3 inflammasome.
DR   PRO; PR:Q9JJ25; -.
DR   Proteomes; UP000002494; Chromosome 10.
DR   Bgee; ENSRNOG00000008134; Expressed in spleen and 12 other tissues.
DR   GO; GO:0005776; C:autophagosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0061702; C:inflammasome complex; ISO:RGD.
DR   GO; GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005875; C:microtubule associated complex; ISS:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
DR   GO; GO:0003779; F:actin binding; ISS:UniProtKB.
DR   GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; NAS:UniProtKB.
DR   GO; GO:0006954; P:inflammatory response; ISS:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR   GO; GO:1900016; P:negative regulation of cytokine production involved in inflammatory response; ISS:UniProtKB.
DR   GO; GO:0050728; P:negative regulation of inflammatory response; ISO:RGD.
DR   GO; GO:0032691; P:negative regulation of interleukin-1 beta production; ISO:RGD.
DR   GO; GO:0032695; P:negative regulation of interleukin-12 production; ISO:RGD.
DR   GO; GO:0071641; P:negative regulation of macrophage inflammatory protein 1 alpha production; ISO:RGD.
DR   GO; GO:1900226; P:negative regulation of NLRP3 inflammasome complex assembly; ISS:UniProtKB.
DR   GO; GO:0010508; P:positive regulation of autophagy; ISS:UniProtKB.
DR   GO; GO:2001056; P:positive regulation of cysteine-type endopeptidase activity; ISO:RGD.
DR   GO; GO:0050729; P:positive regulation of inflammatory response; ISO:RGD.
DR   GO; GO:0032731; P:positive regulation of interleukin-1 beta production; ISO:RGD.
DR   GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR   GO; GO:1904270; P:pyroptosome complex assembly; ISS:UniProtKB.
DR   GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR   GO; GO:0032651; P:regulation of interleukin-1 beta production; ISS:UniProtKB.
DR   GO; GO:0034341; P:response to interferon-gamma; ISS:UniProtKB.
DR   GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
DR   GO; GO:0034021; P:response to silicon dioxide; IEP:RGD.
DR   Gene3D; 1.10.533.10; -; 1.
DR   InterPro; IPR004020; DAPIN.
DR   InterPro; IPR011029; DEATH-like_dom_sf.
DR   InterPro; IPR028841; Pyrin.
DR   InterPro; IPR000315; Znf_B-box.
DR   PANTHER; PTHR24103:SF606; PTHR24103:SF606; 2.
DR   Pfam; PF02758; PYRIN; 1.
DR   Pfam; PF00643; zf-B_box; 1.
DR   SMART; SM00336; BBOX; 1.
DR   SMART; SM01289; PYRIN; 1.
DR   SUPFAM; SSF47986; SSF47986; 1.
DR   PROSITE; PS50824; DAPIN; 1.
DR   PROSITE; PS50119; ZF_BBOX; 1.
PE   2: Evidence at transcript level;
KW   Actin-binding; Cell projection; Coiled coil; Cytoplasm;
KW   Cytoplasmic vesicle; Cytoskeleton; Immunity; Inflammatory response;
KW   Innate immunity; Metal-binding; Microtubule; Nucleus; Reference proteome;
KW   Zinc; Zinc-finger.
FT   CHAIN           1..747
FT                   /note="Pyrin"
FT                   /id="PRO_0000220366"
FT   DOMAIN          1..92
FT                   /note="Pyrin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00061"
FT   ZN_FING         442..479
FT                   /note="B box-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT   REGION          89..181
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          487..645
FT                   /note="Required for homotrimerization and induction of
FT                   pyroptosomes"
FT                   /evidence="ECO:0000250|UniProtKB:O15553"
FT   REGION          698..720
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          479..508
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        149..164
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         442
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT   BINDING         445
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT   BINDING         465
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT   BINDING         471
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT   CONFLICT        122
FT                   /note="P -> L (in Ref. 1; AAF03767)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        200..203
FT                   /note="PQEC -> TPQNA (in Ref. 1; AAF03767)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        211
FT                   /note="R -> RN (in Ref. 1; AAF03767)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        271
FT                   /note="E -> ET (in Ref. 1; AAF03767)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        416
FT                   /note="H -> C (in Ref. 1; AAF03767)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   747 AA;  83743 MW;  72690E5EA0C122E8 CRC64;
     MANTRVDHLL NTLEELLPYE LEKFKFKLHT TSLEKGHSRI PLSLVKMARP IKLTRLLLTY
     YGEEYAVRLT LQILRATNQR QLAEELHKAT GPEHLTEENG VGGSVQSSAE NKDKGVKGSD
     VPGEDEAQQN DDESDILPPI QAEVGKGPQK KSLAKRKDQR GPESLDSQTK PGARSAAPLY
     RRTLVTQSPG DKENRAGAQP QECQLCREAA RSTAMSQGGE RSRRLKCICL QERSDPGVLK
     LPLTQKKENP QIQKLFRLKR KQEMAVSFVR EATLNGRTTG TLEKGVGIPE HSMMLDEETS
     RNMSSKISLT REKRCTASWT ENGNGGPETP ETLGETVSSI LCDSCSPKVL LSLGEKLAQT
     PEDPASLGQA ASKGRSRDKV ACPLCHTQGE LPAKACVQSS CSCSVAPGDP KASGRHSICF
     QCQSSRAGKS CEAQSPQFLP QCPRHMKQVQ LLFCEDHREP ICLICRLSQE HQGHRVRPIE
     EAALQYKEQI RKQLERLREM RGYVEEHKLP ADKKAEDFLK QTETQKQRIS CPLEKLFQFL
     EQQEQLFVTW LQELVQTIGK VRETYYTQVS LLDKLIGELE AKQDQPEWEL MQDIGATLHR
     AETMTASELL GIPPGVKEKL HLLYQKSKSA EKNMQRFSEM LGSEMAFSAS DVATREGCRP
     STTKAQALIP TVHLKCDGAH TQDFDVILCA ELEAGGSEPQ DYLHPSSAQD TPELHEIHSQ
     NNKRKFKSFL KWKPSFSRTD RCLRTCW
 
 
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