ID   MEG1_MAIZE              Reviewed;          88 AA.
AC   Q6JB15; B6SYN5;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   03-SEP-2014, sequence version 2.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=Protein MATERNALLY EXPRESSED GENE 1;
DE   Flags: Precursor;
GN   Name=MEG1; ORFNames=GRMZM2G354335 {ECO:0000305};
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2), TISSUE SPECIFICITY,
RP   DEVELOPMENTAL STAGE, 3D-STRUCTURE MODELING, GLYCOSYLATION, SUBCELLULAR
RP   LOCATION, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=15105441; DOI=10.1105/tpc.019778;
RA   Gutierrez-Marcos J.F., Costa L.M., Biderre-Petit C., Khbaya B.,
RA   O'Sullivan D.M., Wormald M., Perez P., Dickinson H.G.;
RT   "maternally expressed gene1 is a novel maize endosperm transfer cell-
RT   specific gene with a maternal parent-of-origin pattern of expression.";
RL   Plant Cell 16:1288-1301(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. B73;
RX   PubMed=19965430; DOI=10.1126/science.1178534;
RA   Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA   Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA   Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA   Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA   Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA   Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA   Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA   Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA   Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA   Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA   Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA   Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA   Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA   Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA   Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA   Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA   Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA   Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA   Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA   Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA   SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA   Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA   Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA   Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA   Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA   Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT   "The B73 maize genome: complexity, diversity, and dynamics.";
RL   Science 326:1112-1115(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA   Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA   Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT   "Insights into corn genes derived from large-scale cDNA sequencing.";
RL   Plant Mol. Biol. 69:179-194(2009).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=22245001; DOI=10.1016/j.cub.2011.11.059;
RA   Costa L.M., Yuan J., Rouster J., Paul W., Dickinson H.,
RA   Gutierrez-Marcos J.F.;
RT   "Maternal control of nutrient allocation in plant seeds by genomic
RT   imprinting.";
RL   Curr. Biol. 22:160-165(2012).
CC   -!- FUNCTION: Regulates maternal nutrient uptake, sucrose partitioning, and
CC       seed biomass yield. Necessary and sufficient for the establishment and
CC       differentiation of the endosperm nutrient transfer cells located at the
CC       mother:seed interface. Exclusive expression of the maternal allele at
CC       the early stages of endosperm development. The maternal allele is
CC       hypomethylated. At later stages, expression becomes biallelic.
CC       Regulated by the transcription factor MRP1.
CC       {ECO:0000269|PubMed:22245001}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall. Cell membrane. Secreted,
CC       extracellular space, extracellular matrix.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q6JB15-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q6JB15-2; Sequence=VSP_055463;
CC   -!- TISSUE SPECIFICITY: Expressed exclusively in endosperm. Found in basal
CC       endosperm transfer cells. {ECO:0000269|PubMed:15105441}.
CC   -!- DEVELOPMENTAL STAGE: Expressed from 4 to 20 days after pollination.
CC       {ECO:0000269|PubMed:15105441}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:15105441}.
CC   -!- SIMILARITY: Belongs to the MEG family. {ECO:0000305}.
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DR   EMBL; AY536121; AAT09810.1; -; mRNA.
DR   EMBL; AY536120; AAT36287.1; -; Genomic_DNA.
DR   EMBL; AC199699; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; EU957850; ACG29968.1; -; mRNA.
DR   RefSeq; XP_008652138.1; XM_008653916.1. [Q6JB15-1]
DR   AlphaFoldDB; Q6JB15; -.
DR   SMR; Q6JB15; -.
DR   STRING; 4577.GRMZM2G354335_P01; -.
DR   PaxDb; Q6JB15; -.
DR   EnsemblPlants; Zm00001eb302080_T001; Zm00001eb302080_P001; Zm00001eb302080. [Q6JB15-1]
DR   GeneID; 103632056; -.
DR   Gramene; Zm00001eb302080_T001; Zm00001eb302080_P001; Zm00001eb302080. [Q6JB15-1]
DR   KEGG; zma:103632056; -.
DR   OrthoDB; 1650039at2759; -.
DR   Proteomes; UP000007305; Chromosome 7.
DR   ExpressionAtlas; Q6JB15; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell membrane; Cell wall; Developmental protein;
KW   Disulfide bond; Extracellular matrix; Glycoprotein; Membrane;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..88
FT                   /note="Protein MATERNALLY EXPRESSED GENE 1"
FT                   /id="PRO_0000430073"
FT   CARBOHYD        36
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        65..87
FT                   /evidence="ECO:0000250"
FT   DISULFID        68..76
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         27..40
FT                   /note="GNGHVTDDVNVSAP -> G (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15105441"
FT                   /id="VSP_055463"
SQ   SEQUENCE   88 AA;  9810 MW;  17E09BA3C3D6EBAD CRC64;
     MEYKKRVDAL VFFSLLLLGY FAAHAHGNGH VTDDVNVSAP AEEGILREKR AQCAQGFLPC
     KDNKCYCCIG GRTHDCYYTM AQCSHACF