MEGF9_HUMAN
ID MEGF9_HUMAN Reviewed; 602 AA.
AC Q9H1U4; B7Z315; O75098;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 3.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Multiple epidermal growth factor-like domains protein 9;
DE Short=Multiple EGF-like domains protein 9;
DE AltName: Full=Epidermal growth factor-like protein 5;
DE Short=EGF-like protein 5;
DE Flags: Precursor;
GN Name=MEGF9; Synonyms=EGFL5, KIAA0818; ORFNames=UNQ671/PRO1305;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Corpus callosum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-267.
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 228-602.
RC TISSUE=Brain;
RX PubMed=9693030; DOI=10.1006/geno.1998.5341;
RA Nakayama M., Nakajima D., Nagase T., Nomura N., Seki N., Ohara O.;
RT "Identification of high-molecular-weight proteins with multiple EGF-like
RT motifs by motif-trap screening.";
RL Genomics 51:27-34(1998).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- POLYMORPHISM: The poly-Ala stretch (positions 24 to 31) may be
CC polymorphic and varies from 6 to 8 Ala residues. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-9 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ88736.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK295380; BAH12051.1; -; mRNA.
DR EMBL; AL138836; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471090; EAW87465.1; -; Genomic_DNA.
DR EMBL; AY358370; AAQ88736.1; ALT_SEQ; mRNA.
DR EMBL; AB011542; BAA32470.1; -; mRNA.
DR CCDS; CCDS48010.2; -.
DR RefSeq; NP_001073966.2; NM_001080497.2.
DR AlphaFoldDB; Q9H1U4; -.
DR SMR; Q9H1U4; -.
DR BioGRID; 108275; 50.
DR STRING; 9606.ENSP00000363040; -.
DR GlyGen; Q9H1U4; 14 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q9H1U4; -.
DR PhosphoSitePlus; Q9H1U4; -.
DR BioMuta; MEGF9; -.
DR DMDM; 116242627; -.
DR EPD; Q9H1U4; -.
DR jPOST; Q9H1U4; -.
DR MassIVE; Q9H1U4; -.
DR MaxQB; Q9H1U4; -.
DR PaxDb; Q9H1U4; -.
DR PeptideAtlas; Q9H1U4; -.
DR PRIDE; Q9H1U4; -.
DR ProteomicsDB; 80447; -.
DR Antibodypedia; 2992; 158 antibodies from 23 providers.
DR DNASU; 1955; -.
DR Ensembl; ENST00000373930.4; ENSP00000363040.3; ENSG00000106780.9.
DR GeneID; 1955; -.
DR KEGG; hsa:1955; -.
DR MANE-Select; ENST00000373930.4; ENSP00000363040.3; NM_001080497.3; NP_001073966.2.
DR UCSC; uc022bms.2; human.
DR CTD; 1955; -.
DR DisGeNET; 1955; -.
DR GeneCards; MEGF9; -.
DR HGNC; HGNC:3234; MEGF9.
DR HPA; ENSG00000106780; Tissue enhanced (retina).
DR MIM; 604268; gene.
DR neXtProt; NX_Q9H1U4; -.
DR OpenTargets; ENSG00000106780; -.
DR VEuPathDB; HostDB:ENSG00000106780; -.
DR eggNOG; KOG0994; Eukaryota.
DR GeneTree; ENSGT00940000161177; -.
DR HOGENOM; CLU_031674_2_0_1; -.
DR InParanoid; Q9H1U4; -.
DR OMA; SGNCQCR; -.
DR OrthoDB; 619649at2759; -.
DR PhylomeDB; Q9H1U4; -.
DR PathwayCommons; Q9H1U4; -.
DR BioGRID-ORCS; 1955; 8 hits in 1074 CRISPR screens.
DR ChiTaRS; MEGF9; human.
DR GenomeRNAi; 1955; -.
DR Pharos; Q9H1U4; Tdark.
DR PRO; PR:Q9H1U4; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q9H1U4; protein.
DR Bgee; ENSG00000106780; Expressed in olfactory bulb and 202 other tissues.
DR Genevisible; Q9H1U4; HS.
DR GO; GO:0005604; C:basement membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009887; P:animal organ morphogenesis; IBA:GO_Central.
DR GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR GO; GO:0034446; P:substrate adhesion-dependent cell spreading; IBA:GO_Central.
DR GO; GO:0009888; P:tissue development; IBA:GO_Central.
DR CDD; cd00055; EGF_Lam; 5.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR002049; LE_dom.
DR Pfam; PF00053; Laminin_EGF; 5.
DR SMART; SM00181; EGF; 5.
DR SMART; SM00180; EGF_Lam; 5.
DR PROSITE; PS00022; EGF_1; 2.
DR PROSITE; PS01186; EGF_2; 1.
DR PROSITE; PS01248; EGF_LAM_1; 5.
DR PROSITE; PS50027; EGF_LAM_2; 5.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Laminin EGF-like domain; Membrane;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..602
FT /note="Multiple epidermal growth factor-like domains
FT protein 9"
FT /id="PRO_0000007526"
FT TOPO_DOM 31..514
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 515..535
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 536..602
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 204..253
FT /note="Laminin EGF-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DOMAIN 254..300
FT /note="Laminin EGF-like 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DOMAIN 301..348
FT /note="Laminin EGF-like 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DOMAIN 349..399
FT /note="Laminin EGF-like 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DOMAIN 400..451
FT /note="Laminin EGF-like 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT REGION 38..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..120
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 127..159
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 160..174
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..190
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 40
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 182
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 205
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 218
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 245
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 267
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 305
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 428
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 468
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 481
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 500
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 204..217
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 206..224
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 226..235
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 238..251
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 254..266
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 256..272
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 274..283
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 286..298
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 301..310
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 303..317
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 320..329
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 332..346
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 349..360
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 351..371
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 374..383
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 386..397
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 400..415
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 402..422
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 425..434
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DISULFID 437..449
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
SQ SEQUENCE 602 AA; 62984 MW; C2EF73E588C8E375 CRC64;
MNGGAERAMR SLPSLGGLAL LCCAAAAAAA AVASAASAGN VTGGGGAAGQ VDASPGPGLR
GEPSHPFPRA TAPTAQAPRT GPPRATVHRP LAATSPAQSP ETTPLWATAG PSSTTFQAPL
GPSPTTPPAA ERTSTTSQAP TRPAPTTLST TTGPAPTTPV ATTVPAPTTP RTPTPDLPSS
SNSSVLPTPP ATEAPSSPPP EYVCNCSVVG SLNVNRCNQT TGQCECRPGY QGLHCETCKE
GFYLNYTSGL CQPCDCSPHG ALSIPCNSSG KCQCKVGVIG SICDRCQDGY YGFSKNGCLP
CQCNNRSASC DALTGACLNC QENSKGNHCE ECKEGFYQSP DATKECLRCP CSAVTSTGSC
SIKSSELEPE CDQCKDGYIG PNCNKCENGY YNFDSICRKC QCHGHVDPVK TPKICKPESG
ECINCLHNTT GFWCENCLEG YVHDLEGNCI KKEVILPTPE GSTILVSNAS LTTSVPTPVI
NSTFTPTTLQ TIFSVSTSEN STSALADVSW TQFNIIILTV IIIVVVLLMG FVGAVYMYRE
YQNRKLNAPF WTIELKEDNI SFSSYHDSIP NADVSGLLED DGNEVAPNGQ LTLTTPIHNY
KA
