MEGG1_CAEEL
ID MEGG1_CAEEL Reviewed; 636 AA.
AC Q21126;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Protein meg-1 {ECO:0000305};
DE AltName: Full=Maternal effect germ cell defective 1 {ECO:0000303|PubMed:18202375};
GN Name=meg-1 {ECO:0000303|PubMed:18202375, ECO:0000312|WormBase:K02B9.1};
GN ORFNames=K02B9.1 {ECO:0000312|WormBase:K02B9.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=18202375; DOI=10.1534/genetics.107.080218;
RA Leacock S.W., Reinke V.;
RT "MEG-1 and MEG-2 are embryo-specific P-granule components required for
RT germline development in Caenorhabditis elegans.";
RL Genetics 178:295-306(2008).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=21305687; DOI=10.1002/dvg.20726;
RA Kapelle W.S., Reinke V.;
RT "C. elegans meg-1 and meg-2 differentially interact with nanos family
RT members to either promote or inhibit germ cell proliferation and
RT survival.";
RL Genesis 49:380-391(2011).
RN [4] {ECO:0000305}
RP FUNCTION, INTERACTION WITH PPTR-1; PPTR-2 AND PGL-1, DISRUPTION PHENOTYPE,
RP PHOSPHORYLATION AT SER-574, AND MUTAGENESIS OF SER-574.
RX PubMed=25535836; DOI=10.7554/elife.04591;
RA Wang J.T., Smith J., Chen B.C., Schmidt H., Rasoloson D., Paix A.,
RA Lambrus B.G., Calidas D., Betzig E., Seydoux G.;
RT "Regulation of RNA granule dynamics by phosphorylation of serine-rich,
RT intrinsically disordered proteins in C. elegans.";
RL Elife 3:e04591-e04591(2014).
CC -!- FUNCTION: P granule component, which acts redundantly with P granule
CC component meg-2 to promote P granule segregation during embryogenesis,
CC and germ cell proliferation and differentiation in larval stages
CC (PubMed:18202375, PubMed:21305687, PubMed:25535836). In its
CC phosphorylated form, and together with meg-2, promotes the disassembly
CC of zygotic P granules in the anterior cytoplasm of pre-gastrulation
CC embryos (PubMed:25535836). In its dephosphorylated form, and together
CC with meg-2, promotes the assembly and accumulation of zygotic P
CC granules in the posterior cytoplasm of pre-gastrulation embryos
CC (PubMed:25535836). May function with the nanos family members nos-2 and
CC nos-3 to promote germ cell proliferation during larval development
CC (PubMed:21305687). Required for fertility (PubMed:18202375,
CC PubMed:21305687). {ECO:0000269|PubMed:18202375,
CC ECO:0000269|PubMed:21305687, ECO:0000269|PubMed:25535836}.
CC -!- SUBUNIT: Interacts with pptr-1, pptr-2 and pgl-1.
CC {ECO:0000269|PubMed:25535836}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic granule
CC {ECO:0000269|PubMed:18202375}. Note=Localizes to P granules in
CC embryogenesis (PubMed:18202375). Localization to P granules may depend
CC on mes-1 (PubMed:18202375). {ECO:0000269|PubMed:18202375}.
CC -!- TISSUE SPECIFICITY: Not expressed in the adult germline or in any
CC somatic tissues. {ECO:0000269|PubMed:18202375}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the P lineage during embryogenesis
CC (PubMed:18202375). First expressed at the 4- to 8-cell stage, and
CC expression remains throughout the remaining P cell divisions in P2, P3
CC and P4 cells, and subsequently in primordial germ cells Z2 and Z3
CC (PubMed:18202375). Expression begins to diminish at the 100-cell stage
CC (PubMed:18202375). {ECO:0000269|PubMed:18202375}.
CC -!- PTM: Phosphorylated by mbk-2, which promotes the disassembly of zygotic
CC P granules in the anterior cytoplasm of pre-gastrulation embryos
CC (PubMed:25535836). Dephosphorylated by a phosphatase complex containing
CC the PP2A regulatory subunit pptr-1, which promotes the assembly and
CC accumulation of zygotic P granules in the posterior cytoplasm of pre-
CC gastrulation embryos (PubMed:25535836). {ECO:0000269|PubMed:25535836}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in sterility, and
CC in P granule segregation defects (PubMed:18202375). Simultaneous RNAi-
CC mediated knockdown of meg-1 and meg-2 results in defects in P granule
CC disassembly in the anterior cytoplasm of the P1 blastomere causing some
CC P granules to be mis-segregated to somatic blastomeres
CC (PubMed:25535836). {ECO:0000269|PubMed:18202375,
CC ECO:0000269|PubMed:25535836}.
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DR EMBL; BX284606; CAA93511.1; -; Genomic_DNA.
DR PIR; T23232; T23232.
DR RefSeq; NP_510319.1; NM_077918.5.
DR AlphaFoldDB; Q21126; -.
DR SMR; Q21126; -.
DR DIP; DIP-27481N; -.
DR STRING; 6239.K02B9.1; -.
DR iPTMnet; Q21126; -.
DR EPD; Q21126; -.
DR PaxDb; Q21126; -.
DR EnsemblMetazoa; K02B9.1.1; K02B9.1.1; WBGene00010492.
DR GeneID; 181502; -.
DR KEGG; cel:CELE_K02B9.1; -.
DR UCSC; K02B9.1; c. elegans.
DR CTD; 181502; -.
DR WormBase; K02B9.1; CE16206; WBGene00010492; meg-1.
DR GeneTree; ENSGT00970000198175; -.
DR HOGENOM; CLU_430371_0_0_1; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00010492; Expressed in germ line (C elegans) and 3 other tissues.
DR GO; GO:0043186; C:P granule; IDA:WormBase.
DR GO; GO:0036093; P:germ cell proliferation; IGI:WormBase.
DR GO; GO:0032504; P:multicellular organism reproduction; IMP:WormBase.
DR GO; GO:1903864; P:P granule disassembly; IGI:WormBase.
DR GO; GO:0000003; P:reproduction; IMP:WormBase.
PE 1: Evidence at protein level;
KW Developmental protein; Phosphoprotein; Reference proteome.
FT CHAIN 1..636
FT /note="Protein meg-1"
FT /id="PRO_0000456204"
FT MOD_RES 574
FT /note="Phosphoserine; by mbk-2"
FT /evidence="ECO:0000269|PubMed:25535836"
FT MUTAGEN 574
FT /note="S->A: Reduces phosphorylation."
FT /evidence="ECO:0000269|PubMed:25535836"
SQ SEQUENCE 636 AA; 70018 MW; 8ECCABA1901818BF CRC64;
MDNRGHFSSN GNFPPQGYHR REQSQEGMRI GNHHGPFSSG NMRSIGGSAQ NQQQRHWTNM
LNGSNNVENS WVCFSGNTSL ADDPNVLSNF SALAQQRVNH FDTIVQERDN RNASFLNGSA
VGNNLNTSFS VFGNLRGGDQ DHRVLSDHTG IYTGLGSTGQ NAVGTGIRLA ADVANGNFLE
QRAPTAMGHN QSYSALNQSL APTLLDQYNQ ALLDQYNQSS QMAQGRGYPA PNIAYGLQNA
GFPAPQIAHR PNTQNADPQA MNMNNRLRDH TFQMPHTNAQ VPMSSLPGLF NLSMNHGSGN
QQFQMNQSSS GPAPQLPNLS ESFQMAQGSS QVTMSSRPAH SNTSMNHSSR NQYNHVDQRP
SRPAPHLPTL TKEEEAFLET PDFEQFGRQL YNYLLPGILP NGAADTFSDA PKHQLLKLAK
TLKPMLYDYW RKTMQMQNRG ANINIIQWII DFNAKFAALN SSVAKASNSS DVLNQTLPTA
AEVSDVARED ASTSQPSKSR SMYIRPAASL DNTLETLDEN LDSSQSHAGP VPAASTKPKT
PSFEKMIRYS GIKKRSTMDM DNFVRMLDEK INFSPEPSTS SDIASSVKGY MSQSFLHQQD
DEAPDCTKNV HSESDLKQAE PQESDKQSDK ELPSNE
