ID   MEGG2_CAEEL             Reviewed;         819 AA.
AC   Q21127;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 2.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Protein meg-2 {ECO:0000305};
DE   AltName: Full=Maternal effect germ cell defective 2 {ECO:0000303|PubMed:18202375};
GN   Name=meg-2 {ECO:0000303|PubMed:18202375, ECO:0000312|WormBase:K02B9.2};
GN   ORFNames=K02B9.2 {ECO:0000312|WormBase:K02B9.2};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=18202375; DOI=10.1534/genetics.107.080218;
RA   Leacock S.W., Reinke V.;
RT   "MEG-1 and MEG-2 are embryo-specific P-granule components required for
RT   germline development in Caenorhabditis elegans.";
RL   Genetics 178:295-306(2008).
RN   [3] {ECO:0000305}
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=21305687; DOI=10.1002/dvg.20726;
RA   Kapelle W.S., Reinke V.;
RT   "C. elegans meg-1 and meg-2 differentially interact with nanos family
RT   members to either promote or inhibit germ cell proliferation and
RT   survival.";
RL   Genesis 49:380-391(2011).
RN   [4] {ECO:0000305}
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=25535836; DOI=10.7554/elife.04591;
RA   Wang J.T., Smith J., Chen B.C., Schmidt H., Rasoloson D., Paix A.,
RA   Lambrus B.G., Calidas D., Betzig E., Seydoux G.;
RT   "Regulation of RNA granule dynamics by phosphorylation of serine-rich,
RT   intrinsically disordered proteins in C. elegans.";
RL   Elife 3:e04591-e04591(2014).
CC   -!- FUNCTION: P granule component, which acts redundantly with P granule
CC       component meg-1 to promote P granule segregation during embryogenesis,
CC       and germ cell proliferation and differentiation in larval stages
CC       (PubMed:18202375, PubMed:21305687, PubMed:25535836). Together with
CC       phosphorylated meg-1, promotes the disassembly of zygotic P granules in
CC       the anterior cytoplasm of pre-gastrulation embryos (PubMed:25535836).
CC       Together with dephosphorylated meg-1, promotes the assembly and
CC       accumulation of zygotic P granules in the posterior cytoplasm of pre-
CC       gastrulation embryos (PubMed:25535836). Required for fertility
CC       (PubMed:18202375, PubMed:21305687). {ECO:0000269|PubMed:18202375,
CC       ECO:0000269|PubMed:21305687, ECO:0000269|PubMed:25535836}.
CC   -!- INTERACTION:
CC       Q21127; O17670: eya-1; NbExp=3; IntAct=EBI-317734, EBI-311862;
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic granule
CC       {ECO:0000269|PubMed:18202375}. Note=Localizes to P granules in
CC       embryogenesis. {ECO:0000269|PubMed:18202375}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the P lineage during embryogenesis
CC       (PubMed:18202375). Expressed at the 4- to 8-cell stage, and expression
CC       remains throughout the remaining P cell divisions in P2, P3 and P4
CC       cells, and subsequently in primordial germ cells Z2 and Z3
CC       (PubMed:18202375). {ECO:0000269|PubMed:18202375}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in sterility, and
CC       in P granule segregation defects (PubMed:18202375). RNAi-mediated
CC       knockdown increases the sterility phenotype of meg-1 vr10 and meg-1
CC       vr11 mutants from 15 to 100% and from 4 to 93%, respectively, and
CC       results in a larger proportion of under-proliferated gonads than in the
CC       single meg-1 mutants (PubMed:18202375, PubMed:21305687,
CC       PubMed:25535836). Simultaneous RNAi-mediated knockdown of meg-1 and
CC       meg-2 results in defects in P granule disassembly in the anterior
CC       cytoplasm of the P1 blastomere causing some P granules to be mis-
CC       segregated to somatic blastomeres (PubMed:25535836).
CC       {ECO:0000269|PubMed:18202375, ECO:0000269|PubMed:21305687,
CC       ECO:0000269|PubMed:25535836}.
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DR   EMBL; BX284606; CAA93509.2; -; Genomic_DNA.
DR   PIR; T23231; T23231.
DR   RefSeq; NP_510318.2; NM_077917.3.
DR   AlphaFoldDB; Q21127; -.
DR   DIP; DIP-27499N; -.
DR   IntAct; Q21127; 11.
DR   STRING; 6239.K02B9.2; -.
DR   EPD; Q21127; -.
DR   PaxDb; Q21127; -.
DR   EnsemblMetazoa; K02B9.2.1; K02B9.2.1; WBGene00010493.
DR   GeneID; 181501; -.
DR   KEGG; cel:CELE_K02B9.2; -.
DR   UCSC; K02B9.2; c. elegans.
DR   CTD; 181501; -.
DR   WormBase; K02B9.2; CE35572; WBGene00010493; meg-2.
DR   GeneTree; ENSGT00970000198175; -.
DR   HOGENOM; CLU_345214_0_0_1; -.
DR   Proteomes; UP000001940; Chromosome X.
DR   Bgee; WBGene00010493; Expressed in embryo and 2 other tissues.
DR   GO; GO:0043186; C:P granule; IDA:WormBase.
DR   GO; GO:1903864; P:P granule disassembly; IGI:WormBase.
PE   1: Evidence at protein level;
KW   Developmental protein; Reference proteome.
FT   CHAIN           1..819
FT                   /note="Protein meg-2"
FT                   /id="PRO_0000456203"
SQ   SEQUENCE   819 AA;  89393 MW;  61086043B648E1D3 CRC64;
     MENCDDLSSD ENLPPPGDHR REQSQERARV GNRDESVSST NMRSIGGAVH NQQQLYWTDM
     YNGSNNLENF WLCFSGNTSL ADDPTVQSDS SASTQSRTNQ SGTIAQETDN RNASNFPPRS
     TTRRGQTYES RDESQGGQAP SHQNQVPGVD RDEYAGDRSN VSINLPSVDQ WREYSFSDNS
     FQGESRTVQR RSSRATHRVK NQFGSIGDGR TSNNTSLQEP VIQDTRNRNR SESSSTMSSQ
     SRTEHAPIFR LQYNYQREAD NRSSRLVQIQ GHSAPNNANG LQNTGGQAPD IGPEVNGQSS
     DTRAMNMGNQ MAQGDSHVPM NFVPRPLSNG VPRLHGVPRL VDMYDYELAQ RAHARRYPTS
     NMPYGFPNAG FQAPPHMGLW PNNQNADTRA MNMDNRSRDR TFQMPPANAQ VPTSSLSGLF
     NSSINHGSGN QQFQMGQSSS GSAPQLPALP ENFLMAHANS QIPTSSLSGL FNAMNHGSGN
     RMNHSSSGPA PQLPNLPESF LMAHGIGQVE TRSPAGPSNS SINHGSGNQQ FPVQQSSSRP
     APQLPNLPES FLMAHGLSQI QTSSTAGPSN SSMNHGSGNQ QFRMGQSSSR PAPQLPSLQE
     SFLMAHGIAQ VETSSTAGPS NSSMNHGSGN QQFPVQQSSS RPAPHLPDLI RQEEEFLNTL
     EFEQFARRLY NDLLPGVDPN YDSGTSTSET PVLKFSITLK AMLYDYWRTM VQMQYRGANI
     NFLQSIINLN ARSAALNSSN ARASGSADVQ NQTVSATVEG ADDASANVNA LSDLLAQSFP
     RQAEGEASRR NDIDHPEDDL NQADSPKSDN QSDKDPPSN