ID   MEH_CHLAA               Reviewed;         280 AA.
AC   A9WC41;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Mesaconyl-C(4)-CoA hydratase;
DE            EC=4.2.1.153;
DE   AltName: Full=3-methylfumaryl-CoA hydratase;
GN   Name=meh; OrderedLocusNames=Caur_0180;
OS   Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl).
OC   Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Chloroflexineae;
OC   Chloroflexaceae; Chloroflexus.
OX   NCBI_TaxID=324602;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29366 / DSM 635 / J-10-fl;
RX   PubMed=21714912; DOI=10.1186/1471-2164-12-334;
RA   Tang K.H., Barry K., Chertkov O., Dalin E., Han C.S., Hauser L.J.,
RA   Honchak B.M., Karbach L.E., Land M.L., Lapidus A., Larimer F.W.,
RA   Mikhailova N., Pitluck S., Pierson B.K., Blankenship R.E.;
RT   "Complete genome sequence of the filamentous anoxygenic phototrophic
RT   bacterium Chloroflexus aurantiacus.";
RL   BMC Genomics 12:334-334(2011).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ACTIVITY
RP   REGULATION, AND SUBUNIT.
RC   STRAIN=DSM 636 / Ok-70-fl;
RX   PubMed=19955419; DOI=10.1073/pnas.0908356106;
RA   Zarzycki J., Brecht V., Muller M., Fuchs G.;
RT   "Identifying the missing steps of the autotrophic 3-hydroxypropionate CO2
RT   fixation cycle in Chloroflexus aurantiacus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:21317-21322(2009).
CC   -!- FUNCTION: Involved in the glyoxylate assimilation cycle used to
CC       regenerate acetyl-CoA and produce pyruvate as universal precursor for
CC       biosynthesis. Catalyzes the hydration of 3-methylfumaryl-CoA
CC       (mesaconyl-C4-CoA) to (3S)-citramalyl-CoA.
CC       {ECO:0000269|PubMed:19955419}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3S)-citramalyl-CoA = 3-methylfumaryl-CoA + H2O;
CC         Xref=Rhea:RHEA:38271, ChEBI:CHEBI:15377, ChEBI:CHEBI:58668,
CC         ChEBI:CHEBI:75636; EC=4.2.1.153;
CC         Evidence={ECO:0000269|PubMed:19955419};
CC   -!- ACTIVITY REGULATION: Inhibited by 3-methylfumaryl-CoA concentrations
CC       above 0.3 mM. {ECO:0000269|PubMed:19955419}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=75 uM for 3-methylfumaryl-CoA (at 45 degrees Celsius)
CC         {ECO:0000269|PubMed:19955419};
CC         Note=kcat is 1045 sec(-1) for hydratase activity with 3-
CC         methylfumaryl-CoA (at 45 degrees Celsius).;
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:19955419}.
CC   -!- SIMILARITY: Belongs to the HTD2 family. {ECO:0000305}.
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DR   EMBL; CP000909; ABY33434.1; -; Genomic_DNA.
DR   RefSeq; WP_012256090.1; NC_010175.1.
DR   RefSeq; YP_001633823.1; NC_010175.1.
DR   AlphaFoldDB; A9WC41; -.
DR   SMR; A9WC41; -.
DR   STRING; 324602.Caur_0180; -.
DR   EnsemblBacteria; ABY33434; ABY33434; Caur_0180.
DR   KEGG; cau:Caur_0180; -.
DR   PATRIC; fig|324602.8.peg.209; -.
DR   eggNOG; COG3777; Bacteria.
DR   HOGENOM; CLU_028690_3_0_0; -.
DR   InParanoid; A9WC41; -.
DR   OMA; IHYDRRY; -.
DR   BioCyc; MetaCyc:MON-17294; -.
DR   BRENDA; 4.2.1.153; 1352.
DR   Proteomes; UP000002008; Chromosome.
DR   GO; GO:0019171; F:3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity; IBA:GO_Central.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   InterPro; IPR029069; HotDog_dom_sf.
DR   SUPFAM; SSF54637; SSF54637; 2.
PE   1: Evidence at protein level;
KW   Carbon dioxide fixation; Lyase; Reference proteome.
FT   CHAIN           1..280
FT                   /note="Mesaconyl-C(4)-CoA hydratase"
FT                   /id="PRO_0000430342"
SQ   SEQUENCE   280 AA;  31000 MW;  BC192AE560FA20F7 CRC64;
     MSSADWMAWI GRTEQVEDDI CLAQAIAAAA TLEPPSGAPT ADSPLPPLWH WFYFLPRAPQ
     SQLSSDGHPQ RGGFIPPIPY PRRMFAGARI RFHHPLRIGQ PARREGVIRN ITQKSGRSGP
     LAFVTVGYQI YQHEMLCIEE EQDIVYREPG APVPAPTPVE LPPVHDAITR TVVPDPRLLF
     RFSALTFNAH RIHYDRPYAQ HEEGYPGLVV HGPLVAVLLM ELARHHTSRP IVGFSFRSQA
     PLFDLAPFRL LARPNGDRID LEAQGPDGAT ALSATVELGG