ID   MEI2_CAEEL              Reviewed;         280 AA.
AC   O44740;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Meiotic spindle formation protein 2;
GN   Name=mei-2; ORFNames=F57B10.12;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR
RP   LOCATION, AND INTERACTION WITH MEI-1.
RX   PubMed=10809666;
RA   Srayko M., Buster D.W., Bazirgan O.A., McNally F.J., Mains P.E.;
RT   "MEI-1/MEI-2 katanin-like microtubule severing activity is required for
RT   Caenorhabditis elegans meiosis.";
RL   Genes Dev. 14:1072-1084(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Forms a heterodimeric complex in conjunction with mei-1 which
CC       severs microtubules in vitro in an ATP-dependent manner. This activity
CC       may promote rapid reorganization of cellular microtubule arrays. May
CC       act to target mei-1 within the cell. Required specifically for meiotic
CC       spindle formation in the female germline.
CC       {ECO:0000269|PubMed:10809666}.
CC   -!- SUBUNIT: Interacts with mei-1. {ECO:0000269|PubMed:10809666}.
CC   -!- INTERACTION:
CC       O44740; P34808: mei-1; NbExp=3; IntAct=EBI-323243, EBI-323248;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle pole
CC       {ECO:0000269|PubMed:10809666}. Note=Localized to the spindle poles and
CC       condensed chromatin during female meiosis. Also localized to the polar
CC       body. Correct localization requires mei-1.
CC   -!- DEVELOPMENTAL STAGE: Highly expressed in the female germline.
CC       {ECO:0000269|PubMed:10809666}.
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DR   EMBL; AF248052; AAF62184.1; -; mRNA.
DR   EMBL; FO081416; CCD71480.1; -; Genomic_DNA.
DR   PIR; T32747; T32747.
DR   RefSeq; NP_491894.1; NM_059493.6.
DR   AlphaFoldDB; O44740; -.
DR   SMR; O44740; -.
DR   BioGRID; 37825; 15.
DR   ComplexPortal; CPX-3889; Katanin complex.
DR   DIP; DIP-27215N; -.
DR   IntAct; O44740; 4.
DR   STRING; 6239.F57B10.12; -.
DR   EPD; O44740; -.
DR   PaxDb; O44740; -.
DR   PeptideAtlas; O44740; -.
DR   EnsemblMetazoa; F57B10.12.1; F57B10.12.1; WBGene00003184.
DR   GeneID; 172374; -.
DR   KEGG; cel:CELE_F57B10.12; -.
DR   UCSC; F57B10.12; c. elegans.
DR   CTD; 172374; -.
DR   WormBase; F57B10.12; CE11320; WBGene00003184; mei-2.
DR   HOGENOM; CLU_1016461_0_0_1; -.
DR   InParanoid; O44740; -.
DR   OMA; ENKYIRR; -.
DR   OrthoDB; 1876136at2759; -.
DR   PhylomeDB; O44740; -.
DR   PRO; PR:O44740; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00003184; Expressed in germ line (C elegans) and 3 other tissues.
DR   GO; GO:0000794; C:condensed nuclear chromosome; IDA:WormBase.
DR   GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR   GO; GO:0008352; C:katanin complex; IDA:WormBase.
DR   GO; GO:0001940; C:male pronucleus; IDA:WormBase.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0000922; C:spindle pole; IDA:WormBase.
DR   GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR   GO; GO:0051229; P:meiotic spindle disassembly; IC:ComplexPortal.
DR   GO; GO:0000212; P:meiotic spindle organization; IMP:WormBase.
DR   GO; GO:0007019; P:microtubule depolymerization; IDA:WormBase.
DR   InterPro; IPR028021; Katanin_C-terminal.
DR   Pfam; PF13925; Katanin_con80; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Meiosis; Microtubule;
KW   Reference proteome.
FT   CHAIN           1..280
FT                   /note="Meiotic spindle formation protein 2"
FT                   /id="PRO_0000096400"
FT   REGION          1..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..23
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        63..86
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        87..104
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   280 AA;  31458 MW;  8962A88949693054 CRC64;
     MSGLDDRKKL THAKNRKPLN DIPKSAENRP NTRSTSSRRG AEKDVPITFI GSSRTVKADL
     PEFTNTRSRR PLHSESKKEL SRNPVSRGEE HSSSLPKSSP ESSVSVMSSN ASLWSACTEE
     VNKIGVCAKR ESRNLRVYKM KSFTSNMEQI LSNDNQLAPT VIRILNSRNS WCLNSCHACL
     TFIMENITSD NYGKRSACLK ALASITNSLL DTIIGFASTK TRRIGVDVVA EERAAKATEC
     IHNFRKIVKN RDKIYKQIDQ ETIYKLDAIL ERLKKVSSHK