MEI4_YEAST
ID MEI4_YEAST Reviewed; 408 AA.
AC P29467; D3DLU5; P87265;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Meiosis-specific protein MEI4;
GN Name=MEI4; OrderedLocusNames=YER044C-A; ORFNames=YER044BC;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1545815; DOI=10.1128/mcb.12.3.1340-1351.1992;
RA Menees T.M., Ross-Macdonald P.B., Roeder G.S.;
RT "MEI4, a meiosis-specific yeast gene required for chromosome synapsis.";
RL Mol. Cell. Biol. 12:1340-1351(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP INTERACTION WITH MER2 AND REC114.
RX PubMed=14992724; DOI=10.1016/s1097-2765(04)00063-2;
RA Arora C., Kee K., Maleki S., Keeney S.;
RT "Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break
RT formation, independent of its cytoplasmic role in RNA metabolism.";
RL Mol. Cell 13:549-559(2004).
RN [5]
RP IDENTIFICATION IN THE MER2-MEI4-REC114 COMPLEX, FUNCTION OF THE
RP MER2-MEI4-REC114 COMPLEX, INTERACTION WITH MER2 AND REC114, AND SUBCELLULAR
RP LOCATION.
RX PubMed=16783010; DOI=10.1534/genetics.106.058768;
RA Li J., Hooker G.W., Roeder G.S.;
RT "Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for
RT meiotic double-strand break formation.";
RL Genetics 173:1969-1981(2006).
CC -!- FUNCTION: Required for meiotic induction of recombination, viable spore
CC production and chromosome synapsis. Component of the MER2-MEI4-REC114
CC complex which seems to be required for meiotic double-strand break
CC (DSB) formation. {ECO:0000269|PubMed:16783010}.
CC -!- SUBUNIT: Interacts with MER2 and REC114. Component of the MER2-MEI4-
CC REC114 complex. {ECO:0000269|PubMed:14992724,
CC ECO:0000269|PubMed:16783010}.
CC -!- INTERACTION:
CC P29467; P33323: REC104; NbExp=5; IntAct=EBI-2566907, EBI-16412992;
CC P29467; P21651: REC107; NbExp=7; IntAct=EBI-2566907, EBI-10742;
CC P29467; P32841: REC114; NbExp=8; IntAct=EBI-2566907, EBI-14447;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16783010}. Chromosome
CC {ECO:0000269|PubMed:16783010}. Note=Localizes to chromosomes.
CC -!- DEVELOPMENTAL STAGE: Expressed only in meiosis.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M84764; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; M84765; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U18796; AAB64589.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07699.1; -; Genomic_DNA.
DR PIR; S78084; S78084.
DR RefSeq; NP_010963.1; NM_001180023.1.
DR AlphaFoldDB; P29467; -.
DR SMR; P29467; -.
DR BioGRID; 36781; 48.
DR ComplexPortal; CPX-1809; MER2-MEI4-REC114 meiotic recombination initiation complex.
DR IntAct; P29467; 5.
DR STRING; 4932.YER044C-A; -.
DR PaxDb; P29467; -.
DR PRIDE; P29467; -.
DR EnsemblFungi; YER044C-A_mRNA; YER044C-A; YER044C-A.
DR GeneID; 856768; -.
DR KEGG; sce:YER044C-A; -.
DR SGD; S000001954; MEI4.
DR VEuPathDB; FungiDB:YER044C-A; -.
DR eggNOG; ENOG502S67C; Eukaryota.
DR HOGENOM; CLU_061029_0_0_1; -.
DR InParanoid; P29467; -.
DR OMA; YRKFIQC; -.
DR BioCyc; YEAST:G3O-30346-MON; -.
DR PRO; PR:P29467; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P29467; protein.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IGI:SGD.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR025888; MEI4.
DR Pfam; PF13971; Mei4; 1.
PE 1: Evidence at protein level;
KW Chromosome; DNA recombination; Meiosis; Nucleus; Reference proteome;
KW Sporulation.
FT CHAIN 1..408
FT /note="Meiosis-specific protein MEI4"
FT /id="PRO_0000096402"
FT CONFLICT 329
FT /note="P -> T (in Ref. 1; M84764)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 408 AA; 48215 MW; 86CE544E7567FDD6 CRC64;
MSRGKLEDME QKETSEVDWI ICFALIQSRN PTLWKRALSR KKGDVEDVGA LKSEKNLKIN
PRENSKHIYK WVAPFENGFL NNKSLFAHLE PIYNFLCQNK YKSFEDAVGL KELQSFSKDV
STADINNWFL PRYKILLKIL SLKTKEIDFR GLSQVFQTLQ ILLVSHYSHR IDSDSSFKRT
LIDVHVFNFI AKFLFNRILL KKNQNDPKWL QNFYDQGDGK HLCDKVDYKR LCSLHFTLIY
SIINIQLIKI KTNQTFEPQI LKYVSVLKLI EHILIIIESL IHVLIRFVSK HKLICINRKK
AYCRVYLERE LSLKKTYLKN FYSVISGVPE KELGGLLKIL KIVILSLLET FESIEWQHLK
PFLEKFPAHE ISLQKKRKYI QAALLITAER NLIARFRLSR WFNETENI
