MEI5_YEAST
ID MEI5_YEAST Reviewed; 222 AA.
AC P32489; D6W3P6; Q02946;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Meiosis protein 5;
GN Name=MEI5; OrderedLocusNames=YPL121C; ORFNames=LPH6C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Weber L., Byers B.;
RL Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH DMC1 AND SAE3.
RX PubMed=15620352; DOI=10.1016/j.cell.2004.10.031;
RA Hayase A., Takagi M., Miyazaki T., Oshiumi H., Shinohara M., Shinohara A.;
RT "A protein complex containing Mei5 and Sae3 promotes the assembly of the
RT meiosis-specific RecA homolog Dmc1.";
RL Cell 119:927-940(2004).
CC -!- FUNCTION: Involved in meiotic DNA-break repair. Required for the
CC recruitment of DCM1 to meiosis recombination hot spots.
CC {ECO:0000269|PubMed:15620352}.
CC -!- SUBUNIT: Forms a ternary complex with DMC1 and SAE3.
CC -!- INTERACTION:
CC P32489; P25453: DMC1; NbExp=4; IntAct=EBI-10685, EBI-5955;
CC P32489; P89114: SAE3; NbExp=4; IntAct=EBI-10685, EBI-16412526;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15620352}.
CC -!- SIMILARITY: Belongs to the SFR1/MEI5 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L03182; AAA34768.1; -; Genomic_DNA.
DR EMBL; U43503; AAB68241.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11312.1; -; Genomic_DNA.
DR PIR; S62001; S62001.
DR RefSeq; NP_015204.1; NM_001183935.1.
DR AlphaFoldDB; P32489; -.
DR SMR; P32489; -.
DR BioGRID; 36060; 55.
DR ComplexPortal; CPX-1288; MEI5-SAE3 DMC1 recombinase assembly factor complex.
DR DIP; DIP-2964N; -.
DR IntAct; P32489; 5.
DR MINT; P32489; -.
DR STRING; 4932.YPL121C; -.
DR PaxDb; P32489; -.
DR PRIDE; P32489; -.
DR EnsemblFungi; YPL121C_mRNA; YPL121C; YPL121C.
DR GeneID; 855982; -.
DR KEGG; sce:YPL121C; -.
DR SGD; S000006042; MEI5.
DR VEuPathDB; FungiDB:YPL121C; -.
DR eggNOG; ENOG502S2TF; Eukaryota.
DR HOGENOM; CLU_084833_1_0_1; -.
DR InParanoid; P32489; -.
DR OMA; AQMASNY; -.
DR BioCyc; YEAST:G3O-34020-MON; -.
DR PRO; PR:P32489; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P32489; protein.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
DR GO; GO:0000228; C:nuclear chromosome; IDA:ComplexPortal.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0000707; P:meiotic DNA recombinase assembly; IMP:SGD.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR018468; SFR1/Mei5.
DR Pfam; PF10376; Mei5; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; Meiosis; Nucleus; Reference proteome; Sporulation.
FT CHAIN 1..222
FT /note="Meiosis protein 5"
FT /id="PRO_0000096403"
FT CONFLICT 155..156
FT /note="SD -> VT (in Ref. 1; AAA34768)"
FT /evidence="ECO:0000305"
FT CONFLICT 160..162
FT /note="QLS -> RTFR (in Ref. 1; AAA34768)"
FT /evidence="ECO:0000305"
FT CONFLICT 168
FT /note="E -> G (in Ref. 1; AAA34768)"
FT /evidence="ECO:0000305"
FT CONFLICT 217
FT /note="S -> R (in Ref. 1; AAA34768)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 222 AA; 26461 MW; 81F662E1353AEFBB CRC64;
MHNQEEWLDK DKTLVNEEEN TCINHSYTKK DTNNYRVGKS GIKDLKKPTN QKEIAIKNRE
LTKQLTLLRQ ENNHLQQACK ILSENKIIEN RKSIEKWRTI CEMELSFILN STLIKINRMG
GYKDFLEKEM EAKKRRLEYQ IDNGMEDQIC EIKESDDFRQ LSEVEKQEWE SQMNEQLKEL
EKKKIAELEK LNKVLHDSEG KDFGMAELCT RLKLDYSLIF PQ
