MEIG1_MOUSE
ID MEIG1_MOUSE Reviewed; 88 AA.
AC Q61845;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Meiosis-expressed gene 1 protein;
DE AltName: Full=Protein expressed in male leptotene and zygotene spermatocytes 278;
DE Short=MLZ-278;
GN Name=Meig1; Synonyms=Meg1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE
RP SPECIFICITY.
RC STRAIN=Swiss Webster; TISSUE=Testis;
RX PubMed=1390336;
RA Don J., Wolgemuth D.J.;
RT "Identification and characterization of the regulated pattern of expression
RT of a novel mouse gene, meg1, during the meiotic cell cycle.";
RL Cell Growth Differ. 3:495-505(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, INTERACTION WITH PACRG, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=19805151; DOI=10.1073/pnas.0906414106;
RA Zhang Z., Shen X., Gude D.R., Wilkinson B.M., Justice M.J.,
RA Flickinger C.J., Herr J.C., Eddy E.M., Strauss J.F. III;
RT "MEIG1 is essential for spermiogenesis in mice.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:17055-17060(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=20339383; DOI=10.1038/jhg.2010.26;
RA Kogo H., Kowa-Sugiyama H., Yamada K., Bolor H., Tsutsumi M., Ohye T.,
RA Inagaki H., Taniguchi M., Toda T., Kurahashi H.;
RT "Screening of genes involved in chromosome segregation during meiosis I:
RT toward the identification of genes responsible for infertility in humans.";
RL J. Hum. Genet. 55:293-299(2010).
CC -!- FUNCTION: Essential for spermiogenesis. {ECO:0000269|PubMed:1390336,
CC ECO:0000269|PubMed:19805151}.
CC -!- SUBUNIT: Interacts with PACRG. {ECO:0000269|PubMed:19805151}.
CC -!- INTERACTION:
CC Q61845; Q9DAK2: Pacrg; NbExp=3; IntAct=EBI-15805257, EBI-8572392;
CC -!- TISSUE SPECIFICITY: Several isoforms have been identified differing in
CC their 5'-untranslated exons. These isoforms show different tissue
CC expression. Some are expressed in various tissues, including lung,
CC liver, brain, testis, oviduct and oocytes. Some are testis-specific.
CC {ECO:0000269|PubMed:1390336, ECO:0000269|PubMed:19805151,
CC ECO:0000269|PubMed:20339383}.
CC -!- DEVELOPMENTAL STAGE: Detected as early as 6 days after birth and up-
CC regulated during spermatogenesis. {ECO:0000269|PubMed:1390336,
CC ECO:0000269|PubMed:19805151, ECO:0000269|PubMed:20339383}.
CC -!- DISRUPTION PHENOTYPE: Mice are viable, but males are sterile, producing
CC only a few sperm that are morphologically abnormal. Spermatogenesis is
CC dramatically impaired at the stage of elongation and condensation.
CC Spermatozoa exhibit failure of flagellar formation, disorganization of
CC sperm axonemes and deformed heads. {ECO:0000269|PubMed:19805151}.
CC -!- SIMILARITY: Belongs to the MEIG1 family. {ECO:0000305}.
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DR EMBL; X64455; CAA45786.1; -; mRNA.
DR EMBL; BC049538; AAH49538.1; -; mRNA.
DR CCDS; CCDS38038.1; -.
DR PIR; A49012; A49012.
DR RefSeq; NP_032605.1; NM_008579.4.
DR RefSeq; XP_006497370.1; XM_006497307.3.
DR RefSeq; XP_006497371.1; XM_006497308.3.
DR PDB; 2N2Y; NMR; -; A=1-88.
DR PDBsum; 2N2Y; -.
DR AlphaFoldDB; Q61845; -.
DR BMRB; Q61845; -.
DR SMR; Q61845; -.
DR BioGRID; 222579; 1.
DR DIP; DIP-48982N; -.
DR IntAct; Q61845; 1.
DR STRING; 10090.ENSMUSP00000070310; -.
DR PhosphoSitePlus; Q61845; -.
DR PaxDb; Q61845; -.
DR PRIDE; Q61845; -.
DR ProteomicsDB; 295923; -.
DR Antibodypedia; 51531; 85 antibodies from 18 providers.
DR DNASU; 104362; -.
DR Ensembl; ENSMUST00000064685; ENSMUSP00000070310; ENSMUSG00000026650.
DR Ensembl; ENSMUST00000115081; ENSMUSP00000110733; ENSMUSG00000026650.
DR Ensembl; ENSMUST00000115082; ENSMUSP00000110734; ENSMUSG00000026650.
DR Ensembl; ENSMUST00000115083; ENSMUSP00000110735; ENSMUSG00000026650.
DR Ensembl; ENSMUST00000115084; ENSMUSP00000110736; ENSMUSG00000026650.
DR GeneID; 104362; -.
DR KEGG; mmu:104362; -.
DR UCSC; uc008idw.1; mouse.
DR CTD; 644890; -.
DR MGI; MGI:1202878; Meig1.
DR VEuPathDB; HostDB:ENSMUSG00000026650; -.
DR eggNOG; ENOG502S7BQ; Eukaryota.
DR GeneTree; ENSGT00390000013550; -.
DR HOGENOM; CLU_160103_0_0_1; -.
DR InParanoid; Q61845; -.
DR OMA; QVKQVDM; -.
DR OrthoDB; 1489247at2759; -.
DR PhylomeDB; Q61845; -.
DR TreeFam; TF329080; -.
DR BioGRID-ORCS; 104362; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Meig1; mouse.
DR PRO; PR:Q61845; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q61845; protein.
DR Bgee; ENSMUSG00000026650; Expressed in seminiferous tubule of testis and 70 other tissues.
DR ExpressionAtlas; Q61845; baseline and differential.
DR Genevisible; Q61845; MM.
DR GO; GO:0005829; C:cytosol; IDA:MGI.
DR GO; GO:0002177; C:manchette; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:1905198; P:manchette assembly; IMP:MGI.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0008104; P:protein localization; IMP:MGI.
DR GO; GO:0007288; P:sperm axoneme assembly; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR InterPro; IPR020186; Meiosis-expressed_gene_1.
DR PANTHER; PTHR17008; PTHR17008; 1.
DR Pfam; PF15163; Meiosis_expr; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Differentiation; Meiosis; Reference proteome;
KW Spermatogenesis.
FT CHAIN 1..88
FT /note="Meiosis-expressed gene 1 protein"
FT /id="PRO_0000096404"
FT STRAND 10..13
FT /evidence="ECO:0007829|PDB:2N2Y"
FT HELIX 19..30
FT /evidence="ECO:0007829|PDB:2N2Y"
FT HELIX 35..42
FT /evidence="ECO:0007829|PDB:2N2Y"
FT TURN 51..53
FT /evidence="ECO:0007829|PDB:2N2Y"
FT STRAND 56..60
FT /evidence="ECO:0007829|PDB:2N2Y"
FT STRAND 66..68
FT /evidence="ECO:0007829|PDB:2N2Y"
FT HELIX 77..82
FT /evidence="ECO:0007829|PDB:2N2Y"
FT STRAND 83..87
FT /evidence="ECO:0007829|PDB:2N2Y"
SQ SEQUENCE 88 AA; 10823 MW; 7EDB2CF7A31CADC0 CRC64;
MATSDVKPKS ISRAKKWSEE IENLYRFQQA GYRDEIEYKQ VKQVAMVDRW PETGYVKKLQ
RRDNTFFYYN KERECEDKEV HKVKVYVY
