MEII1_MOUSE
ID MEII1_MOUSE Reviewed; 1268 AA.
AC Q9D4I2; B2RQ44; F8WHB9;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2017, sequence version 4.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Meiosis inhibitor protein 1;
DE AltName: Full=Meiosis defective protein 1;
GN Name=Mei1 {ECO:0000312|MGI:MGI:3028590};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAP74551.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=129S1/Sv {ECO:0000312|EMBL:AAP74551.1};
RX PubMed=14668445; DOI=10.1073/pnas.2432067100;
RA Libby B.J., Reinholdt L.G., Schimenti J.C.;
RT "Positional cloning and characterization of Mei1, a vertebrate-specific
RT gene required for normal meiotic chromosome synapsis in mice.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:15706-15711(2003).
RN [2] {ECO:0000305, ECO:0000312|EMBL:BAB30279.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:BAB30279.2};
RC TISSUE=Testis {ECO:0000312|EMBL:BAB30279.2};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAI37750.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5] {ECO:0000305}
RP DISRUPTION PHENOTYPE.
RX PubMed=11820814; DOI=10.1006/dbio.2001.0535;
RA Libby B.J., De La Fuente R., O'Brien M.J., Wigglesworth K., Cobb J.,
RA Inselman A., Eaker S., Handel M.A., Eppig J.J., Schimenti J.C.;
RT "The mouse meiotic mutation mei1 disrupts chromosome synapsis with sexually
RT dimorphic consequences for meiotic progression.";
RL Dev. Biol. 242:174-187(2002).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=15928951; DOI=10.1007/s00412-005-0346-4;
RA Reinholdt L.G., Schimenti J.C.;
RT "Mei1 is epistatic to Dmc1 during mouse meiosis.";
RL Chromosoma 114:127-134(2005).
RN [7]
RP DISRUPTION PHENOTYPE.
RX PubMed=30388401; DOI=10.1016/j.ajhg.2018.10.007;
RA Nguyen N.M.P., Ge Z.J., Reddy R., Fahiminiya S., Sauthier P., Bagga R.,
RA Sahin F.I., Mahadevan S., Osmond M., Breguet M., Rahimi K., Lapensee L.,
RA Hovanes K., Srinivasan R., Van den Veyver I.B., Sahoo T., Ao A.,
RA Majewski J., Taketo T., Slim R.;
RT "Causative mutations and mechanism of androgenetic hydatidiform moles.";
RL Am. J. Hum. Genet. 103:740-751(2018).
CC -!- FUNCTION: Required for normal meiotic chromosome synapsis. May be
CC involved in the formation of meiotic double-strand breaks (DSBs) in
CC spermatocytes. {ECO:0000269|PubMed:14668445,
CC ECO:0000269|PubMed:15928951}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9D4I2-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:14668445, ECO:0000269|PubMed:16141072};
CC IsoId=Q9D4I2-2; Sequence=VSP_052892;
CC -!- TISSUE SPECIFICITY: Strongly expressed in testis, weakly in brain, and
CC not detected in spleen, liver, kidney, small intestine or colon.
CC {ECO:0000269|PubMed:14668445}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in 17 dpc ovaries. Expression is
CC significantly reduced in newborn ovaries, and is undetectable in
CC ovaries from 5 day and 8 week old mice. {ECO:0000269|PubMed:14668445}.
CC -!- DISRUPTION PHENOTYPE: Mice are infertile due to meiotic arrest caused
CC by defects in chromosome synapsis. They lack zygotene Rad51 foci, and
CC are likely to be deficient in meiotic double-strand break (DSB)
CC formation. In addition, mutant spermatocytes exhibit meiotic arrest at
CC entry into pachynema, whereas oocytes progress to an abnormal metaphase
CC I. MEI1-deficient oocytes are capable of fertilization. The zygotes are
CC capable of initiating embryonic development but mostly arrest at the 2-
CC 4 cell stage. Among all the zygotes, approximately 5% are androgenetic
CC and do not contain maternal chromosomes (PubMed:30388401).
CC {ECO:0000269|PubMed:11820814, ECO:0000269|PubMed:14668445,
CC ECO:0000269|PubMed:30388401}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY270177; AAP74551.1; -; mRNA.
DR EMBL; AK016514; BAB30279.2; -; mRNA.
DR EMBL; AC102176; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC137749; AAI37750.1; -; mRNA.
DR CCDS; CCDS79396.1; -. [Q9D4I2-2]
DR CCDS; CCDS88810.1; -. [Q9D4I2-1]
DR RefSeq; NP_001297364.1; NM_001310435.1. [Q9D4I2-2]
DR RefSeq; NP_083173.2; NM_028897.3. [Q9D4I2-1]
DR AlphaFoldDB; Q9D4I2; -.
DR BioGRID; 216697; 1.
DR STRING; 10090.ENSMUSP00000086582; -.
DR iPTMnet; Q9D4I2; -.
DR PhosphoSitePlus; Q9D4I2; -.
DR PaxDb; Q9D4I2; -.
DR PRIDE; Q9D4I2; -.
DR Antibodypedia; 27052; 81 antibodies from 15 providers.
DR Ensembl; ENSMUST00000089178; ENSMUSP00000086582; ENSMUSG00000068117. [Q9D4I2-1]
DR Ensembl; ENSMUST00000188048; ENSMUSP00000139689; ENSMUSG00000068117. [Q9D4I2-2]
DR Ensembl; ENSMUST00000189540; ENSMUSP00000140479; ENSMUSG00000068117. [Q9D4I2-2]
DR GeneID; 74369; -.
DR KEGG; mmu:74369; -.
DR UCSC; uc007wyd.2; mouse. [Q9D4I2-2]
DR CTD; 150365; -.
DR MGI; MGI:3028590; Mei1.
DR VEuPathDB; HostDB:ENSMUSG00000068117; -.
DR eggNOG; ENOG502QV5Z; Eukaryota.
DR GeneTree; ENSGT00390000002077; -.
DR InParanoid; Q9D4I2; -.
DR OMA; GLLCNVE; -.
DR OrthoDB; 67575at2759; -.
DR PhylomeDB; Q9D4I2; -.
DR TreeFam; TF336500; -.
DR BioGRID-ORCS; 74369; 0 hits in 66 CRISPR screens.
DR ChiTaRS; Mei1; mouse.
DR PRO; PR:Q9D4I2; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9D4I2; protein.
DR Bgee; ENSMUSG00000068117; Expressed in lumbar dorsal root ganglion and 24 other tissues.
DR ExpressionAtlas; Q9D4I2; baseline and differential.
DR Genevisible; Q9D4I2; MM.
DR GO; GO:0007276; P:gamete generation; IMP:MGI.
DR GO; GO:0007141; P:male meiosis I; IMP:MGI.
DR GO; GO:0007127; P:meiosis I; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IMP:MGI.
DR GO; GO:0000212; P:meiotic spindle organization; IMP:MGI.
DR GO; GO:0045141; P:meiotic telomere clustering; IMP:MGI.
DR GO; GO:0048477; P:oogenesis; IMP:MGI.
DR GO; GO:0040038; P:polar body extrusion after meiotic divisions; IMP:MGI.
DR GO; GO:0007286; P:spermatid development; IMP:MGI.
DR GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR InterPro; IPR016024; ARM-type_fold.
DR SUPFAM; SSF48371; SSF48371; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Meiosis; Reference proteome.
FT CHAIN 1..1268
FT /note="Meiosis inhibitor protein 1"
FT /id="PRO_0000347265"
FT VAR_SEQ 1..374
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14668445,
FT ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:16141072"
FT /id="VSP_052892"
FT CONFLICT 680
FT /note="A -> V (in Ref. 4; AAI37750)"
FT /evidence="ECO:0000305"
FT CONFLICT 863
FT /note="V -> A (in Ref. 4; AAI37750)"
FT /evidence="ECO:0000305"
FT CONFLICT 866
FT /note="W -> R (in Ref. 4; AAI37750)"
FT /evidence="ECO:0000305"
FT CONFLICT 894
FT /note="A -> S (in Ref. 4; AAI37750)"
FT /evidence="ECO:0000305"
FT CONFLICT 1199
FT /note="L -> R (in Ref. 4; AAI37750)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1268 AA; 140874 MW; 4A63F758A69CE22D CRC64;
MTAADHPAFG RDEEAALLLE RTHHRHDPRW LLPVSPHVCM ACALELLPEP GVSLVRKKHV
VFCFQDALVR HTSLVAQLVA QDQRVCIHFV RVLFGLLNSV EDGSMADLCI EVLVQLTTQP
NMEQTIRCLM NECHRELCNL RSMGGSLATT TLLGKLVDTI PGLADELVME HGNLMEHLLR
GLVYPNEGVQ ASICYLYGKL YSSPTAAEML SGHFREKLCA LFLSTLDSAQ TKDLQINCLG
LLRQLLKYDL FVSLIMNKSV PVEGAESVER PSRETSLPLV LKKFLLSRDE ILQVASSHCI
TAVLVHSPAK HAVAFIHADI PEFLFEHLSS SSEILVWSSY NCLILLAEEP LFFSKCHTVY
GIEAVVRSLQ GSQQMTNTEL HTQGLLLFKE ILTRQPEEIR LFTSSALCRD ASRALQEAVS
SPVLAVAAEA LRAISAFLRK DHQSSLPVQY RALRALLEAM LSRCMEFSQT PLNRRSLGHA
CSRNSEKATL RKGSFLLSTL EGFRNACRLA VEFQGEPSAQ ENPFTAPSAE KEDTLEAFSE
YLLSACDSQC IPMVMRYSEE ATHPKLMEVF LSILHSLFVI IPHMKVKFSR KLADSSFIRL
TLELKARFCS GQSHSSLNQV CSSFLYYMCL NLLSAPEKTE PLSQEELSAV SEFLQHGLPH
ISSRTPESLA FLSDRQYVEA ATRQRQYCIL LLFYLAHIHD DRFVPEAELF VAVQSFLLSL
QDQGECPPPV VCKASMYLLA VCGDKDSALA EAVISAIRKF LEGIPDLRGV YTHHPLLLRF
FLAYPGLMSR FGHRVLELWF SWEESGYENL DDDSSPGRTV FPANLAALFR VLQSTPSILL
ILLDLVYSSP VDTARKVLIV LRVFLWENED VKVGGLIRGH FLLILQRLLV EYGASTSGGN
LPLLLNLLSL VQMRNESEQE LDSMAMKLLH QVSMLCGKCS PAHVDILQPS FNFLYWSLHQ
TTPSSQKRAA AVLLSSTALL ELLEKMLALT WTETGSSPRT PLLSSAWLLT ASFSAQQHNG
NLQVHRTLSV ELNQVLKALS FPKKMSALLS AAILRFLRTA LQQSFSSALV VLVPSGDQPL
STPEDAVLAP LGKSQVLALL IGLQNLLVQK DPLLSQACIG CLEALLDYLH ARSPDIALHV
ASQPWNRFLL FTLLDAGENS FLRPEILRLM TLFVQYRSSC VLSREEVGLI LQGAALVDLS
ALSNDTLQAL HGFLLQVQSM GLLNDQHMTQ TLQSSLEGLC SRTFPAQPLF QDMLCLGGVS
VSQAHIRG
