MEIKN_HUMAN
ID MEIKN_HUMAN Reviewed; 373 AA.
AC A0A087WXM9; A0A0A8IBZ9;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 04-MAR-2015, sequence version 2.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=Meiosis-specific kinetochore protein {ECO:0000303|PubMed:25533956};
GN Name=MEIKIN {ECO:0000303|PubMed:25533956};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=25533956; DOI=10.1038/nature14097;
RA Kim J., Ishiguro K., Nambu A., Akiyoshi B., Yokobayashi S., Kagami A.,
RA Ishiguro T., Pendas A.M., Takeda N., Sakakibara Y., Kitajima T.S.,
RA Tanno Y., Sakuno T., Watanabe Y.;
RT "Meikin is a conserved regulator of meiosis-I-specific kinetochore
RT function.";
RL Nature 517:466-471(2015).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
CC -!- FUNCTION: Key regulator of kinetochore function during meiosis I:
CC required both for mono-orientation of kinetochores on sister
CC chromosomes and protection of centromeric cohesin from separase-
CC mediated cleavage. Acts by facilitating kinetochore mono-orientation
CC during meiosis I, when kinetochores on sister chromosomes face the same
CC direction and are thus captured and pulled by spindle fibers from the
CC same pole. Also required to prevent cleavage of cohesin at centromeres
CC during meiosis I, possibly by acting as a regulator of the shugoshin-
CC dependent protection pathway. Acts in collaboration with PLK1: required
CC for PLK1 enrichment to kinetochores. Not required during meiosis II or
CC mitosis. {ECO:0000250|UniProtKB:Q5F2C3}.
CC -!- SUBUNIT: Interacts with CENPC. Interacts with PLK1; required for
CC recruitment of PLK1 at kinetochores. {ECO:0000250|UniProtKB:Q5F2C3}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere
CC {ECO:0000250|UniProtKB:Q5F2C3}. Chromosome, centromere, kinetochore
CC {ECO:0000250|UniProtKB:Q5F2C3}. Note=Localizes at kinetochores in
CC meiosis I but undetectable in meiosis II.
CC {ECO:0000250|UniProtKB:Q5F2C3}.
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DR EMBL; AB987829; BAP91162.1; -; mRNA.
DR EMBL; AC025772; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC026398; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC034228; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KC877112; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS75292.2; -.
DR RefSeq; NP_001290551.1; NM_001303622.1.
DR AlphaFoldDB; A0A087WXM9; -.
DR SMR; A0A087WXM9; -.
DR IntAct; A0A087WXM9; 8.
DR PhosphoSitePlus; A0A087WXM9; -.
DR BioMuta; MEIKIN; -.
DR MassIVE; A0A087WXM9; -.
DR PeptideAtlas; A0A087WXM9; -.
DR Antibodypedia; 77546; 20 antibodies from 3 providers.
DR DNASU; 728637; -.
DR Ensembl; ENST00000442687.6; ENSP00000488568.1; ENSG00000239642.6.
DR GeneID; 728637; -.
DR KEGG; hsa:728637; -.
DR MANE-Select; ENST00000442687.6; ENSP00000488568.1; NM_001303622.2; NP_001290551.1.
DR UCSC; uc031skz.2; human.
DR CTD; 728637; -.
DR DisGeNET; 728637; -.
DR GeneCards; MEIKIN; -.
DR HGNC; HGNC:51253; MEIKIN.
DR HPA; ENSG00000239642; Tissue enhanced (brain, choroid plexus, testis).
DR MIM; 616223; gene.
DR neXtProt; NX_A0A087WXM9; -.
DR OpenTargets; ENSG00000239642; -.
DR VEuPathDB; HostDB:ENSG00000239642; -.
DR GeneTree; ENSGT00390000016270; -.
DR HOGENOM; CLU_701239_0_0_1; -.
DR OMA; NEFPANT; -.
DR OrthoDB; 1201334at2759; -.
DR PathwayCommons; A0A087WXM9; -.
DR SignaLink; A0A087WXM9; -.
DR BioGRID-ORCS; 728637; 2 hits in 103 CRISPR screens.
DR ChiTaRS; MEIKIN; human.
DR GenomeRNAi; 728637; -.
DR Pharos; A0A087WXM9; Tdark.
DR PRO; PR:A0A087WXM9; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; A0A087WXM9; protein.
DR Bgee; ENSG00000239642; Expressed in right testis and 85 other tissues.
DR ExpressionAtlas; A0A087WXM9; baseline and differential.
DR Genevisible; A0A087WXM9; HS.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; ISS:UniProtKB.
DR GO; GO:0000776; C:kinetochore; ISS:UniProtKB.
DR GO; GO:0016321; P:female meiosis chromosome segregation; ISS:UniProtKB.
DR GO; GO:0045143; P:homologous chromosome segregation; ISS:UniProtKB.
DR GO; GO:0007060; P:male meiosis chromosome segregation; ISS:UniProtKB.
DR GO; GO:0010789; P:meiotic sister chromatid cohesion involved in meiosis I; ISS:UniProtKB.
DR GO; GO:0051754; P:meiotic sister chromatid cohesion, centromeric; ISS:UniProtKB.
DR InterPro; IPR034545; Meikin.
DR PANTHER; PTHR38006; PTHR38006; 1.
PE 2: Evidence at transcript level;
KW Centromere; Chromosome; Chromosome partition; Kinetochore; Meiosis;
KW Reference proteome.
FT CHAIN 1..373
FT /note="Meiosis-specific kinetochore protein"
FT /id="PRO_0000432390"
FT REGION 1..91
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 332..335
FT /note="Required for localization to kinetochores"
FT /evidence="ECO:0000250|UniProtKB:Q5F2C3"
FT MOTIF 275..277
FT /note="POLO box domain (PBD)-binding"
FT /evidence="ECO:0000250|UniProtKB:Q5F2C3"
FT COMPBIAS 1..17
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 35..55
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 373 AA; 40829 MW; 6ED94C880370D246 CRC64;
MWPLRVYTRK KREGQRLNLT PTPDLGSPAK AEAPPGSKRK GKVHGLSKIA EKAERSRQGG
SGSGPFSPRL GVTGEKSLQE NRSSEDTQDE KIASLRESVT DDLQVDSSSS NSELVSGLSL
HHGMASSLLS YSVTDSYAEY KSFEESFPSP ELFRKSDYLD WECPNLEEHM QWKNSTLLDT
SKAVAIEKAP QFSNVSAIFS TSSEDYQKCH RKTVMTVADQ NVSPKAKCAS NSESDNAACE
ILLAEKTCPS TPEKTKKKKT NSSTPGKKNR GLLTSTPSSE TAGFVIDLSS VQKASFEELF
PNVSNYVNSN EIVPVSSLQE NSSNEFPANA SEICCIIRTS PGTRQVKNKG VIVKKKKYSL
PKDTPQDIII KMA
