MEIKN_MOUSE
ID MEIKN_MOUSE Reviewed; 434 AA.
AC Q5F2C3; Q3KNB2;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Meiosis-specific kinetochore protein {ECO:0000303|PubMed:25533956};
GN Name=Meikin {ECO:0000303|PubMed:25533956};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND
RP INTERACTION WITH PLK1 ANS CENPC.
RX PubMed=25533956; DOI=10.1038/nature14097;
RA Kim J., Ishiguro K., Nambu A., Akiyoshi B., Yokobayashi S., Kagami A.,
RA Ishiguro T., Pendas A.M., Takeda N., Sakakibara Y., Kitajima T.S.,
RA Tanno Y., Sakuno T., Watanabe Y.;
RT "Meikin is a conserved regulator of meiosis-I-specific kinetochore
RT function.";
RL Nature 517:466-471(2015).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Key regulator of kinetochore function during meiosis I:
CC required both for mono-orientation of kinetochores on sister
CC chromosomes and protection of centromeric cohesin from separase-
CC mediated cleavage. Acts by facilitating kinetochore mono-orientation
CC during meiosis I, when kinetochores on sister chromosomes face the same
CC direction and are thus captured and pulled by spindle fibers from the
CC same pole. Also required to prevent cleavage of cohesin at centromeres
CC during meiosis I, possibly by acting as a regulator of the shugoshin-
CC dependent protection pathway. Acts in collaboration with PLK1: required
CC for PLK1 enrichment to kinetochores. Not required during meiosis II or
CC mitosis. {ECO:0000269|PubMed:25533956}.
CC -!- SUBUNIT: Interacts with CENPC. Interacts with PLK1; required for
CC recruitment of PLK1 at kinetochores. {ECO:0000269|PubMed:25533956}.
CC -!- INTERACTION:
CC Q5F2C3; P49452: Cenpc; NbExp=5; IntAct=EBI-20739301, EBI-1186252;
CC Q5F2C3; Q07832: Plk1; NbExp=4; IntAct=EBI-20739301, EBI-2552999;
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere
CC {ECO:0000269|PubMed:25533956}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:25533956}. Note=Localizes at kinetochores in
CC meiosis I but undetectable in meiosis II.
CC {ECO:0000269|PubMed:25533956}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5F2C3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5F2C3-2; Sequence=VSP_057509;
CC -!- TISSUE SPECIFICITY: Germ cell-specific. Expressed in both testis and
CC ovary. Not expressed in other tissues. {ECO:0000269|PubMed:25533956}.
CC -!- DEVELOPMENTAL STAGE: Appears at centromeres during the pachytene stage,
CC when homologous chromosomes are synapsed. Peaks during diplotene stage,
CC persists with gradual reduction until metaphase I, and disappears in
CC anaphase I. Does not reappear on chromatin in meiosis II.
CC {ECO:0000269|PubMed:25533956}.
CC -!- DISRUPTION PHENOTYPE: Infertility due to defects in meiosis. Mice
CC develop normally and show no visible phenotype but are completely
CC infertile (both males and females) due to defects in meiotic chromosome
CC segregation during meiosis I. Monooriented kinetochores split
CC prematurely and sister chromosomes separate entirely before anaphase of
CC meiosis II. {ECO:0000269|PubMed:25533956}.
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DR EMBL; AB987828; BAP91161.1; -; mRNA.
DR EMBL; AL596127; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC107368; AAI07369.1; -; mRNA.
DR CCDS; CCDS48798.1; -. [Q5F2C3-1]
DR RefSeq; NP_083381.1; NM_029105.2. [Q5F2C3-1]
DR AlphaFoldDB; Q5F2C3; -.
DR IntAct; Q5F2C3; 56.
DR STRING; 10090.ENSMUSP00000091745; -.
DR iPTMnet; Q5F2C3; -.
DR PhosphoSitePlus; Q5F2C3; -.
DR PaxDb; Q5F2C3; -.
DR PRIDE; Q5F2C3; -.
DR ProteomicsDB; 295872; -. [Q5F2C3-1]
DR ProteomicsDB; 295873; -. [Q5F2C3-2]
DR Antibodypedia; 77546; 20 antibodies from 3 providers.
DR Ensembl; ENSMUST00000094193; ENSMUSP00000091745; ENSMUSG00000020332. [Q5F2C3-1]
DR GeneID; 74847; -.
DR KEGG; mmu:74847; -.
DR UCSC; uc007ixv.3; mouse. [Q5F2C3-1]
DR CTD; 728637; -.
DR MGI; MGI:1922097; Meikin.
DR VEuPathDB; HostDB:ENSMUSG00000020332; -.
DR eggNOG; ENOG502S9GN; Eukaryota.
DR GeneTree; ENSGT00390000016270; -.
DR HOGENOM; CLU_701239_0_0_1; -.
DR InParanoid; Q5F2C3; -.
DR OMA; NEFPANT; -.
DR PhylomeDB; Q5F2C3; -.
DR TreeFam; TF338954; -.
DR BioGRID-ORCS; 74847; 3 hits in 73 CRISPR screens.
DR PRO; PR:Q5F2C3; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5F2C3; protein.
DR Bgee; ENSMUSG00000020332; Expressed in animal zygote and 8 other tissues.
DR Genevisible; Q5F2C3; MM.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:MGI.
DR GO; GO:0000776; C:kinetochore; IDA:UniProtKB.
DR GO; GO:0016321; P:female meiosis chromosome segregation; IMP:MGI.
DR GO; GO:0045143; P:homologous chromosome segregation; IMP:MGI.
DR GO; GO:0007060; P:male meiosis chromosome segregation; IMP:MGI.
DR GO; GO:0010789; P:meiotic sister chromatid cohesion involved in meiosis I; IMP:MGI.
DR GO; GO:0051754; P:meiotic sister chromatid cohesion, centromeric; IMP:MGI.
DR InterPro; IPR034545; Meikin.
DR PANTHER; PTHR38006; PTHR38006; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Centromere; Chromosome; Chromosome partition;
KW Kinetochore; Meiosis; Reference proteome.
FT CHAIN 1..434
FT /note="Meiosis-specific kinetochore protein"
FT /id="PRO_0000432391"
FT REGION 1..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 249..289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 391..394
FT /note="Required for localization to kinetochores"
FT /evidence="ECO:0000305|PubMed:25533956"
FT REGION 404..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 334..336
FT /note="POLO box domain (PBD)-binding"
FT /evidence="ECO:0000305|PubMed:25533956"
FT COMPBIAS 1..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 47..61
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..99
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 272..289
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..108
FT /note="Missing (in isoform 2)"
FT /id="VSP_057509"
FT CONFLICT 303
FT /note="A -> S (in Ref. 3; AAI07369)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 434 AA; 47423 MW; CE2F1CB44293D81C CRC64;
MDKIWHMGPR GDYTRKKRAG ERLNLTPKPD LALPGRTEAL PGLKGKGKEQ GLRKITEKKE
LSRLTGSSSQ RPSLLSVTGG EHLQENSPGQ ETPEEKTPPC ETVTDTFEMD SLLSSTELVS
GPAEQDDFSS CLPSCSNAEL HTESTDERGS SFPSPELFRG SDCLDWEHPK LEDYMFYKNS
TLLDTSKAVV IEKAPQFANL SAVLSSSSKN YEKRHRKIGM TLAAQHLSPE PKYASNLASV
VDNAASEVVF AEKTGPPTTE KTQKKPENES EDSGPLVQTK LSSGHPDNKA LCSPLSSALE
STAVRYTLLP QPLEPVLKKG CILPDKQSKA LLTSTPSSDI AEFVIDLSPV QNVSFEELFP
NVSNYVNSSE VVPVSSLQES SSNEFSPNTS EICCIIRSSP GTRQMRRKDP AVKNRCSPPK
DVPLDIIMKT NGRT
