ID   MEIOB_RAT               Reviewed;         470 AA.
AC   B0BMX9;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Meiosis-specific with OB domain-containing protein {ECO:0000305};
DE            EC=3.1.-.-;
GN   Name=Meiob {ECO:0000312|RGD:1583204};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Single-stranded DNA-binding protein required for homologous
CC       recombination in meiosis I. Required for double strand breaks (DSBs)
CC       repair and crossover formation and promotion of faithful and complete
CC       synapsis. Not required for the initial loading of recombinases but
CC       required to maintain a proper number of RAD51 and DMC1 foci after the
CC       zygotene stage. May act by ensuring the stabilization of recombinases,
CC       which is required for successful homology search and meiotic
CC       recombination. Displays Single-stranded DNA 3'-5' exonuclease activity
CC       in vitro. {ECO:0000250|UniProtKB:Q9D513}.
CC   -!- SUBUNIT: Component of a multiprotein complex with RPA2 and SPATA22.
CC       Interacts with the complex BRME1:HSF2BP:BRCA2.
CC       {ECO:0000250|UniProtKB:Q9D513}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9D513}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9D513}. Chromosome
CC       {ECO:0000250|UniProtKB:Q9D513}. Note=Co-localizes with the RPA complex
CC       on meiotic chromosome axes. Accumulates on resected DNA. Localization
CC       is dependent on SPATA22. {ECO:0000250|UniProtKB:Q9D513}.
CC   -!- SIMILARITY: Belongs to the MEIOB family. {ECO:0000305}.
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DR   EMBL; BC158604; AAI58605.1; -; mRNA.
DR   RefSeq; NP_001108505.1; NM_001115033.1.
DR   RefSeq; XP_006246093.1; XM_006246031.3.
DR   AlphaFoldDB; B0BMX9; -.
DR   SMR; B0BMX9; -.
DR   STRING; 10116.ENSRNOP00000057290; -.
DR   PaxDb; B0BMX9; -.
DR   PRIDE; B0BMX9; -.
DR   Ensembl; ENSRNOT00000060551; ENSRNOP00000057290; ENSRNOG00000014711.
DR   GeneID; 685099; -.
DR   KEGG; rno:685099; -.
DR   CTD; 254528; -.
DR   RGD; 1583204; Meiob.
DR   eggNOG; KOG0851; Eukaryota.
DR   GeneTree; ENSGT00390000001723; -.
DR   HOGENOM; CLU_042457_2_0_1; -.
DR   InParanoid; B0BMX9; -.
DR   OMA; SCTLIYE; -.
DR   OrthoDB; 615895at2759; -.
DR   PhylomeDB; B0BMX9; -.
DR   TreeFam; TF323670; -.
DR   PRO; PR:B0BMX9; -.
DR   Proteomes; UP000002494; Chromosome 10.
DR   Bgee; ENSRNOG00000014711; Expressed in testis and 4 other tissues.
DR   GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR   GO; GO:0008310; F:single-stranded DNA 3'-5' exodeoxyribonuclease activity; ISS:UniProtKB.
DR   GO; GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; ISS:UniProtKB.
DR   GO; GO:0007144; P:female meiosis I; ISS:UniProtKB.
DR   GO; GO:0009566; P:fertilization; ISS:UniProtKB.
DR   GO; GO:0007129; P:homologous chromosome pairing at meiosis; ISS:UniProtKB.
DR   GO; GO:0007141; P:male meiosis I; ISO:RGD.
DR   GO; GO:0007140; P:male meiotic nuclear division; ISS:UniProtKB.
DR   GO; GO:0000712; P:resolution of meiotic recombination intermediates; ISS:UniProtKB.
DR   Gene3D; 2.40.50.140; -; 3.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   SUPFAM; SSF50249; SSF50249; 3.
PE   2: Evidence at transcript level;
KW   Chromosome; Cytoplasm; DNA-binding; Exonuclease; Hydrolase; Meiosis;
KW   Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..470
FT                   /note="Meiosis-specific with OB domain-containing protein"
FT                   /id="PRO_0000337137"
FT   DNA_BIND        167..272
FT                   /note="OB"
SQ   SEQUENCE   470 AA;  52819 MW;  89463E920A2CE599 CRC64;
     MAKFFALKNF TALSDLHPNM ANLKIIGIVI GKTDVKGFPD RKNIGSERYT FSFTIRDSPN
     HFVNVSSWGS EDYIRSLSDN FKVGECVIIE NPLIQRKETE REERFSPATP SNYKLLLSEN
     HSMVKVCSPY EVDTKLLSLI HLPVKESRDY YSLADIVANG HSLDGRIINV LAAVRSVGEP
     KYFTTSDRRK GQRCEVKLFD ETEPSFTMTC WDNESILLAQ SWMARETVIF ASDVRINFNK
     FQNCMAATVI SKTIITVNPD TPEANILLNF IRENKETSIA DEIDSYLKES VNLNTIVNVY
     TVEQLKGKAL ENEGKVDPFY GILYAYISTL NIDDETTKVV RNRCSSCGYI VNDASNTCTI
     CSKDSSRSRS FCLSFDVLVD LTDHTGTLRS CSLSGSVAEE TLGCTINEFL TMTSEQKTKL
     KWQLLLERSK IYLKLILSHR ARGGLKVTIL SCKLADPIEA SRDLAGQGHT