MEIOC_MOUSE
ID MEIOC_MOUSE Reviewed; 965 AA.
AC A2AG06; Q3V2K8;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Meiosis-specific coiled-coil domain-containing protein MEIOC {ECO:0000305};
DE AltName: Full=Meiosis-specific with coiled-coil domain protein {ECO:0000303|PubMed:26742488, ECO:0000312|MGI:MGI:2686410};
GN Name=Meioc {ECO:0000312|MGI:MGI:2686410}; Synonyms=Gm1564;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 57-583 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH YTHDC2,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=26742488; DOI=10.1038/ncomms10324;
RA Abby E., Tourpin S., Ribeiro J., Daniel K., Messiaen S., Moison D.,
RA Guerquin J., Gaillard J.C., Armengaud J., Langa F., Toth A., Martini E.,
RA Livera G.;
RT "Implementation of meiosis prophase I programme requires a conserved
RT retinoid-independent stabilizer of meiotic transcripts.";
RL Nat. Commun. 7:10324-10324(2016).
RN [4]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, FUNCTION,
RP IDENTIFICATION BY MASS SPECTROMETRY, AND INTERACTION WITH YTHDC2.
RX PubMed=28380054; DOI=10.1371/journal.pgen.1006704;
RA Soh Y.Q.S., Mikedis M.M., Kojima M., Godfrey A.K., de Rooij D.G.,
RA Page D.C.;
RT "Meioc maintains an extended meiotic prophase I in mice.";
RL PLoS Genet. 13:E1006704-E1006704(2017).
RN [5]
RP INTERACTION WITH YTHDC2.
RX PubMed=29033321; DOI=10.1016/j.molcel.2017.09.021;
RA Wojtas M.N., Pandey R.R., Mendel M., Homolka D., Sachidanandam R.,
RA Pillai R.S.;
RT "Regulation of m6A transcripts by the 3'-5' RNA helicase YTHDC2 is
RT essential for a successful meiotic program in the mammalian germline.";
RL Mol. Cell 68:374-387(2017).
CC -!- FUNCTION: Is required for meiosis completion in both male and female
CC germ cells. Confers stability to numerous meiotic mRNAs in gonads
CC allowing proper initiation and progression into meiosis prophase I. The
CC function may involve YTHDC2 and is independent of induction by retinoic
CC acid (RA). Maintains an extended meiotic prophase I by properly
CC promoting the transition from a mitotic to a meiotic cell cycle program
CC by binding transcripts through its interaction with YTHDC2 that
CC regulate the mitotic cell cycle (PubMed:28380054).
CC {ECO:0000269|PubMed:26742488, ECO:0000269|PubMed:28380054}.
CC -!- SUBUNIT: Interacts with YTHDC2; binds transcripts that regulate the
CC mitotic cell cycle inhibiting progression into metaphase, thereby
CC allowing meiotic prophase to proceed normally (PubMed:26742488,
CC PubMed:28380054, PubMed:29033321). {ECO:0000269|PubMed:26742488,
CC ECO:0000269|PubMed:28380054, ECO:0000269|PubMed:29033321}.
CC -!- INTERACTION:
CC A2AG06; B2RR83: Ythdc2; NbExp=4; IntAct=EBI-11664020, EBI-8572369;
CC A2AG06; Q9H6S0: YTHDC2; Xeno; NbExp=2; IntAct=EBI-11664020, EBI-1057466;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:26742488,
CC ECO:0000269|PubMed:28380054}. Nucleus {ECO:0000269|PubMed:28380054}.
CC Note=at late pachytene a fraction is nuclear.
CC {ECO:0000269|PubMed:28380054}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A2AG06-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2AG06-2; Sequence=VSP_038974;
CC -!- TISSUE SPECIFICITY: Expressed specifically in fetal ovary and postnatal
CC and adult testes (at protein level) (PubMed:26742488). In adult testis
CC expressed in spermatocytes, beginning in preleptotene and extending
CC through most stages of meiotic prophase I, including leptotene,
CC zygotene, and pachytene (PubMed:28380054).
CC {ECO:0000269|PubMed:26742488, ECO:0000269|PubMed:28380054}.
CC -!- DEVELOPMENTAL STAGE: In the ovary appears at 13.5 dpc and disappears
CC shortly after. In postnatal testes, expression increases between 5 and
CC 10 dpp and is maintained through adulthood. In male and female germal
CC cells present from preleptotene to diplotene stages of meiosis prophase
CC I. {ECO:0000269|PubMed:26742488, ECO:0000269|PubMed:28380054}.
CC -!- DISRUPTION PHENOTYPE: Normal growth but mice are fully infertile with
CC barely detectable ovaries and testes at adulthood. Complete absence of
CC follicles in the ovaries and spermatozoon in the seminiferous tubules
CC and epidymides. In testis, approximately 60% of the tubules contain
CC spermatocytes arrested early in meiosis prophase I, the rest contains
CC only spermatogonia. Testes completely lack postmeiotic germ cells, and
CC are depleted for meiotic germ cells. In some individuals, germ cells
CC does not progress past preleptotene, while in others, germ cells
CC advanced to the zygotene stage of meiotic prophase (PubMed:28380054).
CC {ECO:0000269|PubMed:26742488, ECO:0000269|PubMed:28380054}.
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DR EMBL; AL672269; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK131737; BAE20789.1; -; mRNA.
DR CCDS; CCDS48945.1; -. [A2AG06-1]
DR RefSeq; NP_001121048.1; NM_001127576.2. [A2AG06-1]
DR AlphaFoldDB; A2AG06; -.
DR SMR; A2AG06; -.
DR BioGRID; 234510; 1.
DR IntAct; A2AG06; 27.
DR STRING; 10090.ENSMUSP00000097947; -.
DR iPTMnet; A2AG06; -.
DR PhosphoSitePlus; A2AG06; -.
DR PaxDb; A2AG06; -.
DR PRIDE; A2AG06; -.
DR ProteomicsDB; 295874; -. [A2AG06-1]
DR ProteomicsDB; 295875; -. [A2AG06-2]
DR Antibodypedia; 17505; 40 antibodies from 15 providers.
DR Ensembl; ENSMUST00000100378; ENSMUSP00000097947; ENSMUSG00000051455. [A2AG06-1]
DR GeneID; 268491; -.
DR KEGG; mmu:268491; -.
DR UCSC; uc011yfv.2; mouse. [A2AG06-1]
DR CTD; 284071; -.
DR MGI; MGI:2686410; Meioc.
DR VEuPathDB; HostDB:ENSMUSG00000051455; -.
DR eggNOG; ENOG502QPMP; Eukaryota.
DR GeneTree; ENSGT00390000003267; -.
DR HOGENOM; CLU_015343_0_0_1; -.
DR InParanoid; A2AG06; -.
DR OMA; TSFYDCY; -.
DR OrthoDB; 127098at2759; -.
DR PhylomeDB; A2AG06; -.
DR TreeFam; TF313161; -.
DR BioGRID-ORCS; 268491; 6 hits in 71 CRISPR screens.
DR ChiTaRS; Meioc; mouse.
DR PRO; PR:A2AG06; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; A2AG06; protein.
DR Bgee; ENSMUSG00000051455; Expressed in spermatocyte and 27 other tissues.
DR ExpressionAtlas; A2AG06; baseline and differential.
DR Genevisible; A2AG06; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0070192; P:chromosome organization involved in meiotic cell cycle; IMP:UniProtKB.
DR GO; GO:0006302; P:double-strand break repair; IMP:UniProtKB.
DR GO; GO:0007144; P:female meiosis I; IMP:UniProtKB.
DR GO; GO:0051729; P:germline cell cycle switching, mitotic to meiotic cell cycle; IMP:UniProtKB.
DR GO; GO:0007141; P:male meiosis I; IMP:UniProtKB.
DR GO; GO:0051310; P:metaphase plate congression; IMP:UniProtKB.
DR GO; GO:0048255; P:mRNA stabilization; IMP:UniProtKB.
DR GO; GO:0048599; P:oocyte development; IMP:UniProtKB.
DR GO; GO:0007286; P:spermatid development; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR GO; GO:0007130; P:synaptonemal complex assembly; IMP:UniProtKB.
DR InterPro; IPR027963; MEIOC.
DR PANTHER; PTHR33861; PTHR33861; 1.
DR Pfam; PF15189; MEIOC; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..965
FT /note="Meiosis-specific coiled-coil domain-containing
FT protein MEIOC"
FT /id="PRO_0000393412"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 946..965
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..56
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_038974"
SQ SEQUENCE 965 AA; 108830 MW; D1DBA1EA7412DD62 CRC64;
MEVSGGDTCR PRHPQGLREG PEPKVAAAAA AFRGSANRCW NLSVDTSNRL SDVFNSMMLT
GSAPFYDCYK SQNEDNVDLR QTCTPLSSST EYASSIDSSL FYAPWSTYGD DIKQPPSSQI
SVKNRIQTER NDYGSETDLY GLVSNILEEQ DKSQPYFAEG TCSSNLKSVW PMNTSRFVDH
HDLLTEPKRP VDTSISQQAF YSGESVSAVE KQYLHNSSLT PQQKIDELYH GYTGLDLEEQ
WLYLSRSDHS NCYNSQANDT VKATFQEYPF VKNCFTPQTG LSDIMKESGI DTYAYGREKI
CTKGLETPLQ HKRAEIFLSQ FNRYNENADY CRYPEYAHPN KAKLNKCSNF SVQDGKKLAN
GTPETPTVEA DAYTKLFQVK PANQKKMEET IPDQQNFAFP KTTPHLTEKQ FAKEAAFTAD
FGLKSEYGLK PHTACPTNND FANVSEKQQF AKPDPLNSEY FKSVNLFSNS ATSSGGISLN
RPTWMNVQTK NNLPIPYRNQ GNLMKLNSHL SAASKGSNHS SDFPQLSSTN LTSNSNLFQK
YCQENPSAFS SFDFSYNGAE RIQSVNHMEG LTKTGEDNLF ESVTEKKIKQ PNGFCDSYSA
SQYGIIENVN KHNFQAKPQS GHYDPEDIPK HFDGLPQNTY QDLLESQGHF NSHRQGSGDN
NINSRVNRTQ ASCFSNNYMM GDLRHNQGFQ QLGSNGFPLR STHPFGHSVV PLLDSYDLFS
YDDLSHLYPY FNDMMYGDNS FSGFVPTFGF QRPIKTRSGP ASELHIRLEE CYEQWRALEK
ERKKTELALA KNYPGKKVSS TNNTPIPRLT SNPSRVDRLI VDELREQARV VTLLGKMERL
RSSPLHANIS TALDRHLESI HIVQSRRKDE IVNASNRQRQ GVPRCQDDRD VFALATAIKE
MCVATRKART TLWCALQMTL PKTASTAGQA DMEKAFQDLV NCEEKVHESI NSSNPMNQRG
ETSKH
