MEIOS_MOUSE
ID MEIOS_MOUSE Reviewed; 589 AA.
AC A0A5K7RLP0; D3YZT1;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2020, sequence version 1.
DT 03-AUG-2022, entry version 13.
DE RecName: Full=Meiosis initiator protein {ECO:0000305};
GN Name=Meiosin {ECO:0000303|PubMed:32032549};
GN Synonyms=Bhmg1 {ECO:0000312|MGI:MGI:3647482};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:BBD04307.1};
RN [1] {ECO:0000312|EMBL:BBD04307.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH STRA8,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=32032549; DOI=10.1016/j.devcel.2020.01.010;
RA Ishiguro K.I., Matsuura K., Tani N., Takeda N., Usuki S., Yamane M.,
RA Sugimoto M., Fujimura S., Hosokawa M., Chuma S., Ko M.S.H., Araki K.,
RA Niwa H.;
RT "MEIOSIN Directs the Switch from Mitosis to Meiosis in Mammalian Germ
RT Cells.";
RL Dev. Cell 52:429-445.e10(2020).
RN [2] {ECO:0000312|Proteomes:UP000000589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J {ECO:0000312|Proteomes:UP000000589};
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3] {ECO:0007744|PubMed:21183079}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Gatekeeper of meiotic initiation in both male and female germ
CC cells (PubMed:32032549). In complex with STRA8, directly activates the
CC transcription of a subset of critical meiotic genes playing a central
CC role in cell-cycle switching from mitosis to meiosis. Temporal
CC expression of MEIOSIN is required for meiotic entry decision
CC (PubMed:32032549). {ECO:0000269|PubMed:32032549}.
CC -!- SUBUNIT: Interacts with STRA8. {ECO:0000269|PubMed:32032549}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:32032549}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A0A5K7RLP0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A0A5K7RLP0-2; Sequence=VSP_060768;
CC -!- TISSUE SPECIFICITY: Specifically expressed in adult testis and
CC embryonic ovary. {ECO:0000269|PubMed:32032549}.
CC -!- DEVELOPMENTAL STAGE: Induced in 13.5 dpc in the embryonic ovary,
CC expression persists at least until 18.5 dpc (PubMed:32032549). In
CC testes, expressed in spermatocytes during the preleptotene stage in the
CC stage VII-VIII seminiferous tubules (at protein level)
CC (PubMed:32032549). {ECO:0000269|PubMed:32032549}.
CC -!- DISRUPTION PHENOTYPE: Mutant mice develop normally. Males show defects
CC in reproductive organs with smaller-than-normal testes
CC (PubMed:32032549). They have severely impaired spermatogenesis and the
CC absence of postmeiotic spermatids or sperm in testes and epididymis
CC (PubMed:32032549). Female ovaries are degenerated with apparently fewer
CC mature follicles at the age of 4 weeks old (PubMed:32032549).
CC {ECO:0000269|PubMed:32032549}.
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DR EMBL; LC371381; BBD04307.1; -; mRNA.
DR EMBL; AC145199; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_006540527.1; XM_006540464.2.
DR RefSeq; XP_011249055.1; XM_011250753.2. [A0A5K7RLP0-2]
DR AlphaFoldDB; A0A5K7RLP0; -.
DR PhosphoSitePlus; A0A5K7RLP0; -.
DR ProteomicsDB; 310668; -.
DR Ensembl; ENSMUST00000141380; ENSMUSP00000115575; ENSMUSG00000085601. [A0A5K7RLP0-2]
DR GeneID; 243866; -.
DR CTD; 388553; -.
DR MGI; MGI:3647482; Meiosin.
DR VEuPathDB; HostDB:ENSMUSG00000085601; -.
DR eggNOG; KOG0527; Eukaryota.
DR GeneTree; ENSGT00570000080851; -.
DR HOGENOM; CLU_2326153_0_0_1; -.
DR OMA; KQYIRAC; -.
DR OrthoDB; 1031202at2759; -.
DR BioGRID-ORCS; 243866; 0 hits in 34 CRISPR screens.
DR Proteomes; UP000000589; Chromosome 7.
DR Bgee; ENSMUSG00000085601; Expressed in testis and 37 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0090427; P:activation of meiosis; IMP:UniProtKB.
DR GO; GO:0071300; P:cellular response to retinoic acid; IMP:UniProtKB.
DR GO; GO:0051321; P:meiotic cell cycle; IMP:UniProtKB.
DR GO; GO:0048477; P:oogenesis; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Meiosis; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..589
FT /note="Meiosis initiator protein"
FT /id="PRO_0000451166"
FT DOMAIN 14..68
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DNA_BIND 484..552
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 14..27
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 28..68
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 89..191
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 342..363
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 422..457
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 544..566
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..31
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 96..123
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..190
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 422..454
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 403..425
FT /note="Missing (in isoform 2)"
FT /id="VSP_060768"
SQ SEQUENCE 589 AA; 65090 MW; 3EC75E53C0CE0E33 CRC64;
MLGSDKFSCF SDQHRARSPS PTDRKDKKNH TNKLRELALL IPVTMKTRDK KYTKKEILLR
VLHYIQYLQR NIDMTKALLK LHSSNGKGRF VGPGLNPSAG QTQQQHSTPS SSQKPSLWST
SSKPRKKKFT RVSEHPSWPY NPRRSLALDQ AENPNTIHPG LKEENEECAT YPGVLSPSTY
PTTEPSVSEG DGQGAQLVFL DMAQNIFAYD ILSDHAVEVQ GGEPNADIKV QRSFFLTRAQ
PCVSSCRQKL FLCTSSEADK EAPDSDPWLP VWTSEDSPNG SPLALGSSQI NTWHVADYLN
EILGVSSSLF SSPSKILPDH VLEDGTYFLT EGLLESSPAT CEVESPQEKE VSSEGPTGPP
NFQSSVSLDH CYLSLSENVK VLSNCGSSSE STDTESLWGQ EEVRGVATYP RRTGVFSLAQ
PSVLQQANPE GLQTSSDEDR DYTWTPTGQS SGLPVASKKI KKVQASQGPV KPKDSRKACP
GQVKKKCVNG FIMFCRMNRK QYIRACPGTA STAATKDLAQ LWRGMTLEEK KPYCTKARRF
SRQNNRIVKQ ENSSSEDDDG ETPKPFYQLL AEKAQVSLGL TSLPTPNCQ
