MEIS1_XENLA
ID MEIS1_XENLA Reviewed; 390 AA.
AC P79937; P79938;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Homeobox protein Meis1;
DE Short=XMeis1;
GN Name=meis1;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Oocyte;
RX PubMed=9049632; DOI=10.1101/gr.7.2.142;
RA Steelman S., Moskow J.J., Muzynski K., North C., Druck T., Montgomery J.C.,
RA Huebner K., Daar I.O., Buchberg A.M.;
RT "Identification of a conserved family of Meis1-related homeobox genes.";
RL Genome Res. 7:142-156(1997).
RN [2]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11313877; DOI=10.1038/sj.onc.1204250;
RA Maeda R., Mood K., Jones T.L., Aruga J., Buchberg A.M., Daar I.O.;
RT "Xmeis1, a protooncogene involved in specifying neural crest cell fate in
RT Xenopus embryos.";
RL Oncogene 20:1329-1342(2001).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH PBX1 ISOFORM B.
RX PubMed=11960001; DOI=10.1073/pnas.082654899;
RA Maeda R., Ishimura A., Mood K., Park E.K., Buchberg A.M., Daar I.O.;
RT "Xpbx1b and Xmeis1b play a collaborative role in hindbrain and neural crest
RT gene expression in Xenopus embryos.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:5448-5453(2002).
RN [4]
RP FUNCTION.
RX PubMed=16631621; DOI=10.1016/j.bbrc.2006.03.235;
RA Kelly L.E., Carrel T.L., Herman G.E., El-Hodiri H.M.;
RT "Pbx1 and Meis1 regulate activity of the Xenopus laevis Zic3 promoter
RT through a highly conserved region.";
RL Biochem. Biophys. Res. Commun. 344:1031-1037(2006).
CC -!- FUNCTION: Induces expression of a number of neural crest marker genes
CC as part of a heterodimer with isoform b of pbx1, to specify neural
CC crest cell fate. Binds to a highly conserved region in the promoter of
CC the neural crest marker gene zic3. {ECO:0000269|PubMed:11313877,
CC ECO:0000269|PubMed:11960001, ECO:0000269|PubMed:16631621}.
CC -!- SUBUNIT: Interacts with pbx1 isoform b. {ECO:0000269|PubMed:11960001}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11960001}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:11960001}.
CC Note=Relocation from the cytoplasm to the nucleus is dependent on
CC interaction with pbx1 isoform b.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist.;
CC Name=1; Synonyms=XMeis1-1, a;
CC IsoId=P79937-1; Sequence=Displayed;
CC Name=2; Synonyms=XMeis1-2, b;
CC IsoId=P79937-2; Sequence=VSP_002241;
CC -!- TISSUE SPECIFICITY: In the embryo, displays a broad expression pattern
CC with high levels observed in tissues of neural cell fate such as
CC midbrain, hindbrain, dorsal portion of the neural tube, and neural
CC crest-derived branchial arches. Widely expressed in the adult with
CC highest levels in brain and spleen. {ECO:0000269|PubMed:11313877}.
CC -!- DEVELOPMENTAL STAGE: Levels of isoform 1 decrease slightly during
CC gastrulation but show a marked increase during neurulation and
CC throughout embryogenesis. Expression of isoform 2 is low during early
CC embryogenesis but increases after tailbud stages.
CC {ECO:0000269|PubMed:11313877}.
CC -!- SIMILARITY: Belongs to the TALE/MEIS homeobox family. {ECO:0000305}.
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DR EMBL; U68386; AAB19196.1; -; mRNA.
DR EMBL; U68387; AAB19197.1; -; mRNA.
DR RefSeq; NP_001084104.1; NM_001090635.1.
DR AlphaFoldDB; P79937; -.
DR SMR; P79937; -.
DR GeneID; 399305; -.
DR KEGG; xla:399305; -.
DR CTD; 399305; -.
DR Xenbase; XB-GENE-479443; meis1.S.
DR OrthoDB; 1126341at2759; -.
DR Proteomes; UP000186698; Chromosome 5S.
DR Bgee; 399305; Expressed in pancreas and 19 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IGI:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0014036; P:neural crest cell fate specification; IGI:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR032453; PKNOX/Meis_N.
DR Pfam; PF05920; Homeobox_KN; 1.
DR Pfam; PF16493; Meis_PKNOX_N; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Cytoplasm; Developmental protein;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription.
FT CHAIN 1..390
FT /note="Homeobox protein Meis1"
FT /id="PRO_0000049107"
FT DOMAIN 108..191
FT /note="MEIS N-terminal"
FT /evidence="ECO:0000255"
FT DNA_BIND 272..334
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 188..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..329
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250|UniProtKB:O00470"
FT COMPBIAS 226..260
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 261..276
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 373..390
FT /note="PMSGMGMNMGMEGQWHYM -> LQGMPGSYISPSGPMGMSMAQPSYTTSQMP
FT LHHAQLRHGTSVHTYIPGHHHHPAMMMHGGPPQPGMPISASSPSVLNTGDPSMSGHVMN
FT IHAQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:9049632"
FT /id="VSP_002241"
FT VARIANT 34
FT /note="K -> Q"
FT VARIANT 91
FT /note="F -> L"
FT VARIANT 178
FT /note="E -> K"
FT VARIANT 273
FT /note="N -> H"
FT VARIANT 275
FT /note="G -> K"
FT VARIANT 315
FT /note="Missing"
SQ SEQUENCE 390 AA; 42887 MW; D0D98637D08CF32F CRC64;
MAQRYDDLPH YGGMDGVGLP SSMYGDPHAA RSMKSVHHLN HGPPLHPHQY PHSAHTNAMP
PSMGSSVNDA LKRDKDSIYG HPLFPLLALI FEKCELATCT PREPGVAGGD VCSSESFNED
IAVFSKQIRA EKPLFSSNPE LDNLMIQAIQ VLRFHLLELE KVHELCDNFC HRYISCLEGK
MPIDLVIDDR DGGSKSDSED LTRSAPLTDQ PSWSRDHDDA ASIRSGGTPG PSSGGHTSHS
GDNSSEQGDG LDNSIASPST GDDDDPDKEK KRNKGRGIFP KVATNIMRAW LFQHLTHPYP
SEEQKKQLAQ DTGLTILQVN NWFINARRRI VQPMIDQSNR AVSQGTPYNP DGQPIGGFVM
DGQQHMGIRA PGPMSGMGMN MGMEGQWHYM
