MEL1_ORYSJ
ID MEL1_ORYSJ Reviewed; 1058 AA.
AC Q851R2; A0A0P0W4J0; Q0DMP1;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Protein argonaute MEL1;
DE AltName: Full=Protein MEIOSIS ARRESTED AT LEPTOTENE 1;
DE Short=OsMEL1;
GN Name=MEL1; Synonyms=MEAL1; OrderedLocusNames=Os03g0800200, LOC_Os03g58600;
GN ORFNames=OSJNBa0052F07.11;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. Nipponbare; TISSUE=Flower;
RX PubMed=17675402; DOI=10.1105/tpc.107.053199;
RA Nonomura K., Morohoshi A., Nakano M., Eiguchi M., Miyao A., Hirochika H.,
RA Kurata N.;
RT "A germ cell specific gene of the ARGONAUTE family is essential for the
RT progression of premeiotic mitosis and meiosis during sporogenesis in
RT rice.";
RL Plant Cell 19:2583-2594(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18826656; DOI=10.1186/1471-2164-9-451;
RA Kapoor M., Arora R., Lama T., Nijhawan A., Khurana J.P., Tyagi A.K.,
RA Kapoor S.;
RT "Genome-wide identification, organization and phylogenetic analysis of
RT dicer-like, argonaute and RNA-dependent RNA polymerase gene families and
RT their expression analysis during reproductive development and stress in
RT rice.";
RL BMC Genomics 9:451-451(2008).
CC -!- FUNCTION: Essential for the progression of premeiotic mitosis and
CC meiosis during sporogenesis. Regulates the cell division of premeiotic
CC germ cells, the proper modification of meiotic chromosomes, and the
CC faithful progression of meiosis, probably via small RNA-mediated gene
CC silencing. May be involved in histone H3 'Lys-9' demethylation in the
CC pericentromeric region. {ECO:0000269|PubMed:17675402}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed specifically in male and female
CC archesporial cells and sporogenous cells (SC) before meiosis but not in
CC the nursery cells supporting SC. {ECO:0000269|PubMed:17675402}.
CC -!- DISRUPTION PHENOTYPE: Arrest of meiosis in male and female germ cells,
CC causing seed sterility. {ECO:0000269|PubMed:17675402}.
CC -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF13497.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB297928; BAF80153.1; -; mRNA.
DR EMBL; AC104321; AAO37538.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF99382.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF13497.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014959; BAS86885.1; -; Genomic_DNA.
DR RefSeq; XP_015629235.1; XM_015773749.1.
DR AlphaFoldDB; Q851R2; -.
DR SMR; Q851R2; -.
DR STRING; 4530.OS03T0800200-00; -.
DR PaxDb; Q851R2; -.
DR PRIDE; Q851R2; -.
DR EnsemblPlants; Os03t0800200-01; Os03t0800200-01; Os03g0800200.
DR GeneID; 4334449; -.
DR Gramene; Os03t0800200-01; Os03t0800200-01; Os03g0800200.
DR KEGG; osa:4334449; -.
DR eggNOG; KOG1041; Eukaryota.
DR HOGENOM; CLU_004544_0_1_1; -.
DR InParanoid; Q851R2; -.
DR OMA; IHDEIFT; -.
DR OrthoDB; 159407at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q851R2; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IMP:Gramene.
DR GO; GO:0005731; C:nucleolus organizer region; IMP:Gramene.
DR GO; GO:0004521; F:endoribonuclease activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0035197; F:siRNA binding; ISS:Gramene.
DR GO; GO:0007143; P:female meiotic nuclear division; IMP:Gramene.
DR GO; GO:0033169; P:histone H3-K9 demethylation; IDA:Gramene.
DR GO; GO:0007140; P:male meiotic nuclear division; IMP:Gramene.
DR GO; GO:0009561; P:megagametogenesis; IMP:Gramene.
DR GO; GO:0055046; P:microgametogenesis; IMP:Gramene.
DR GO; GO:2000616; P:negative regulation of histone H3-K9 acetylation; IMP:CACAO.
DR GO; GO:0061866; P:negative regulation of histone H3-S10 phosphorylation; IMP:CACAO.
DR GO; GO:1900111; P:positive regulation of histone H3-K9 dimethylation; IMP:CACAO.
DR GO; GO:1903343; P:positive regulation of meiotic DNA double-strand break formation; IMP:CACAO.
DR GO; GO:0035194; P:post-transcriptional gene silencing by RNA; IBA:GO_Central.
DR CDD; cd04657; Piwi_ago-like; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR014811; ArgoL1.
DR InterPro; IPR032472; ArgoL2.
DR InterPro; IPR032473; Argonaute_Mid_dom.
DR InterPro; IPR032474; Argonaute_N.
DR InterPro; IPR003100; PAZ_dom.
DR InterPro; IPR036085; PAZ_dom_sf.
DR InterPro; IPR003165; Piwi.
DR InterPro; IPR045246; Piwi_ago-like.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF08699; ArgoL1; 1.
DR Pfam; PF16488; ArgoL2; 1.
DR Pfam; PF16487; ArgoMid; 1.
DR Pfam; PF16486; ArgoN; 1.
DR Pfam; PF02170; PAZ; 1.
DR Pfam; PF02171; Piwi; 1.
DR SMART; SM01163; DUF1785; 1.
DR SMART; SM00949; PAZ; 1.
DR SMART; SM00950; Piwi; 1.
DR SUPFAM; SSF101690; SSF101690; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
DR PROSITE; PS50821; PAZ; 1.
DR PROSITE; PS50822; PIWI; 1.
PE 2: Evidence at transcript level;
KW Meiosis; Nucleus; Reference proteome; RNA-mediated gene silencing.
FT CHAIN 1..1058
FT /note="Protein argonaute MEL1"
FT /id="PRO_0000378442"
FT DOMAIN 406..520
FT /note="PAZ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00142"
FT DOMAIN 696..1016
FT /note="Piwi"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00150"
FT REGION 1..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 115..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..74
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1058 AA; 117034 MW; 96E62DA4984E1F42 CRC64;
MAYRGGGRGG RGGEQRPPYS GRGDVPGRGG GGGGGGAPPY RPASGFVWPP PGMTPRPGPP
QPQYPRPGPP AVVYGAPMPA AHHQGAYQPG GVYRAPSPGV PVIGGYARST PVTIRAPPPS
HSSAPAPYQP AAAAPAPSSS STAPSATALA KEFEQKLFVS ETALAPPAAV ASAAAAPAGE
ASVESDKDLA PVSKKGLAHP ARPGFGAAGK KVMIRANHFL VNVADNNLFH YDVSINPESK
SRATNREVLN ELIKLHGKTS LGGKLPAYDG RKSLYTAGSL PFESEEFVVK LIDPEKKDKE
RAEREYKITI RIAGRTDLYH LQQFLLGRQR DMPQETIQVL DVVLRESPSW NYVTVSRSFF
STQFGHRGDI GEGLECWRGY YQSLRPTQMG LSLNIDISAT SFFKPVTVIQ FVEEFLNIRD
TSRPLSDRDR VKIKKALRGV RIETNHQEDQ IRRYKITGIT PIPMSQLIFP VDDNGTRKTV
VQYFWDRYNY RLKYASWPCL QSGSDSRPVY LPMEVCKIVE GQRYSKKLND KQVTNILRAT
CQRPQQREQS IHEMVLHNKY TEDRFAQEFG IKVCNDLVSV PARVLPPPML KYHDSGREKT
CAPSVGQWNM INKKMINGGT VDNWTCLSFS RMRPEEVQRF CGDLIQMCNA TGMSFNPRPV
VDVRSTNPNN IENALRDVHR RTSELLAREG KGGLQLLIVI LPEVSGSYGK IKRVCETDLG
IVSQCCLPRH ASRPNKQYLE NVALKINVKV GGRNTVLERA FIRNGIPFVS EVPTIIFGAD
VTHPPPGEDS ASSIAAVVAS MDWPEITKYR GLVSAQPHRQ EIIEDLFSVG KDPVKVVNGG
MIRELLIAFR KKTGRRPERI IFYRDGVSEG QFSHVLLHEM DAIRKACASL EEGYLPPVTF
VVVQKRHHTR LFPEVHGRRD MTDKSGNILP GTVVDRQICH PTEFDFYLCS HAGIQGTSRP
THYHVLYDEN HFTADALQSL TNNLCYTYAR CTRAVSVVPP AYYAHLAAFR ARYYVEGESS
DGGSTPGSSG QAVAREGPVE VRQLPKIKEN VKDVMFYC
