ID   MELA_SHECO              Reviewed;         346 AA.
AC   P23996;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Protein MelA;
GN   Name=melA;
OS   Shewanella colwelliana (Alteromonas colwelliana).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=23;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1756973; DOI=10.1016/0378-1119(91)90598-6;
RA   Fuqua W.C., Coyne V.E., Stein D.C., Lin C.-M., Weiner R.M.;
RT   "Characterization of melA: a gene encoding melanin biosynthesis from the
RT   marine bacterium Shewanella colwelliana.";
RL   Gene 109:131-136(1991).
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC       Note=Binds 1 Fe cation per subunit. {ECO:0000250};
CC   -!- PATHWAY: Pigment biosynthesis; melanin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- INDUCTION: By L-tyrosine.
CC   -!- SIMILARITY: Belongs to the 4HPPD family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M59289; AAA26510.1; -; Genomic_DNA.
DR   PIR; JQ1388; JQ1388.
DR   RefSeq; WP_028762012.1; NZ_BPEU01000001.1.
DR   AlphaFoldDB; P23996; -.
DR   SMR; P23996; -.
DR   STRING; 23.BEL05_04305; -.
DR   UniPathway; UPA00785; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003868; F:4-hydroxyphenylpyruvate dioxygenase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009072; P:aromatic amino acid family metabolic process; IEA:InterPro.
DR   GO; GO:0042438; P:melanin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd07250; HPPD_C_like; 1.
DR   CDD; cd08342; HPPD_N_like; 1.
DR   Gene3D; 3.10.180.10; -; 2.
DR   InterPro; IPR005956; 4OHPhenylPyrv_dOase.
DR   InterPro; IPR041735; 4OHPhenylPyrv_dOase_C.
DR   InterPro; IPR041736; 4OHPhenylPyrv_dOase_N.
DR   InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR   InterPro; IPR004360; Glyas_Fos-R_dOase_dom.
DR   InterPro; IPR037523; VOC.
DR   PANTHER; PTHR11959; PTHR11959; 1.
DR   Pfam; PF00903; Glyoxalase; 1.
DR   PIRSF; PIRSF009283; HPP_dOase; 1.
DR   SUPFAM; SSF54593; SSF54593; 1.
DR   TIGRFAMs; TIGR01263; 4HPPD; 1.
DR   PROSITE; PS51819; VOC; 2.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Iron; Melanin biosynthesis; Metal-binding; Repeat.
FT   CHAIN           1..346
FT                   /note="Protein MelA"
FT                   /id="PRO_0000088411"
FT   DOMAIN          12..141
FT                   /note="VOC 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT   DOMAIN          155..305
FT                   /note="VOC 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT   BINDING         158
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         237
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         314
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   346 AA;  39453 MW;  C5239DBBDE7D95A7 CRC64;
     MASEQNPLGL LGIEFTEFAT PDLDFMHKVF IDFGFSKLKK HKQKDIVYYK QNDINFLLNN
     EKQGFSAQFA KTHGPAISSM GWRVEDANFA FEGAVARGAK PAADEVKDLP YPAIYGIGDS
     LIYFIDTFGD DNNIYTSDFE ALDEPIITQE KGFIEVDHLT NNVHKGTMEY WSNFYKDIFG
     FTEVRYFDIK GSQTALISYA LRSPDGSFCI PINEGKGDDR NQIDEYLKEY DGPGVQHLAF
     RSRDIVASLD AMEGSSIQTL DIIPEYYDTI FEKLPQVTED RDRIKHHQIL VDGDEDGYLL
     QIFTKNLFGP IFIEIIQRKN NLGFGEGNFK ALFESIERDQ VRRGVL