MELB_KLEPN
ID MELB_KLEPN Reviewed; 471 AA.
AC Q02581;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Melibiose permease {ECO:0000305};
DE AltName: Full=Melibiose carrier {ECO:0000303|PubMed:1339436};
DE AltName: Full=Melibiose transporter;
GN Name=melB {ECO:0000303|PubMed:1339436};
OS Klebsiella pneumoniae.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=2002;
RX PubMed=1339436; DOI=10.1016/s0021-9258(19)36971-6;
RA Hama H., Wilson T.H.;
RT "Primary structure and characteristics of the melibiose carrier of
RT Klebsiella pneumoniae.";
RL J. Biol. Chem. 267:18371-18376(1992).
CC -!- FUNCTION: Mediates the transport of melibiose and other galactosides by
CC a symport mechanism. Can use protons and lithium as coupling cations
CC for cotransport, depending on the sugar substrate. It catalyzes proton-
CC melibiose, lithium-lactose, and proton/lithium-methyl-1-thio-beta-D-
CC galactopyranoside (TMG) cotransport. This protein seems to be lacking
CC the ability to recognize sodium cations. {ECO:0000269|PubMed:1339436}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + melibiose(in) = H(+)(out) + melibiose(out);
CC Xref=Rhea:RHEA:28855, ChEBI:CHEBI:15378, ChEBI:CHEBI:28053;
CC Evidence={ECO:0000269|PubMed:1339436};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P02921}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P02921}.
CC -!- SIMILARITY: Belongs to the sodium:galactoside symporter (TC 2.A.2)
CC family. {ECO:0000305}.
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DR EMBL; M97257; AAA25067.1; -; Genomic_DNA.
DR PIR; B44166; B44166.
DR RefSeq; WP_019724884.1; NZ_WXZN01000001.1.
DR AlphaFoldDB; Q02581; -.
DR SMR; Q02581; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0006814; P:sodium ion transport; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR039672; MFS_2.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR001927; Na/Gal_symport.
DR InterPro; IPR018043; Na/Gal_symport_CS.
DR PANTHER; PTHR11328; PTHR11328; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00792; gph; 1.
DR PROSITE; PS00872; NA_GALACTOSIDE_SYMP; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Sugar transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..471
FT /note="Melibiose permease"
FT /id="PRO_0000170755"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..36
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 58..79
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 101..106
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..149
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 171..175
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..234
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 256..266
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..296
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 318..323
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 345..373
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..411
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 433..471
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 471 AA; 52329 MW; 6D373D09BF4BAEC7 CRC64;
MSISMTTKLS YGFGAFGKDF AIGIVYMYLM YYYTDIVGLS VGVVGTLFLV ARILDAIADP
IMGWIVNCTR SRWGKFKPWI LIGTITNSVV LYMLFSAHHF SGGALLAWVW LTYLLWGFTY
TIMDVPFWSL VPTITLDKRE REQLVPYPRF FASLAGFVTA GVTLPFVNAV GGADRGFGFQ
MFTLVLIAFF VVSTLVTLRN VHEVYSSDSG VSEDSSHLSL RQMVALIYKN DQLACLLGMA
LAYNTAANII AGFAIYYFTY VIGSAEMFPY YMSYAGAANL LTLILFPRLV KGLSRRILWA
GASIMPVLGC GVLLLMALGG VYNIALISLA GVLLNIGTAL FWVLQVIMVA DTVDYGEYTM
NIRCESIAYS VQTLVVKAGS AFAAWFIAIV LGIIGYVPNV VQSSHTLLGM QAIMIALPTL
FFALTLFLYF RYYKLNGDML RRIQIHLLDK YRRVPENDVE PERPIVVPNQ V
