MELC_BACSU
ID MELC_BACSU Reviewed; 276 AA.
AC O34518; Q795Q7;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Melibiose/raffinose/stachyose import permease protein MelC {ECO:0000305};
GN Name=melC {ECO:0000303|PubMed:31138628}; Synonyms=amyC;
GN OrderedLocusNames=BSU30290;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT 200 kb rrnB-dnaB region.";
RL Microbiology 143:3431-3441(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=168 / KM0;
RX PubMed=31138628; DOI=10.1128/jb.00109-19;
RA Morabbi Heravi K., Watzlawick H., Altenbuchner J.;
RT "The melREDCA operon encodes a utilization system for the raffinose family
RT of oligosaccharides in Bacillus subtilis.";
RL J. Bacteriol. 201:E00109-E00109(2019).
CC -!- FUNCTION: Part of the ABC transporter complex MelEDC-MsmX involved in
CC melibiose, raffinose and stachyose import. Probably responsible for the
CC translocation of the substrate across the membrane.
CC {ECO:0000269|PubMed:31138628}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (MsmX),
CC two transmembrane proteins (MelC and MelD) and a solute-binding protein
CC (MelE). {ECO:0000269|PubMed:31138628}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:31138628};
CC Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC -!- INDUCTION: Repressed by the transcriptional regulator MelR. Induced by
CC melibiose and raffinose. {ECO:0000269|PubMed:31138628}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene abolishes induction in the
CC presence of melibiose and raffinose. {ECO:0000269|PubMed:31138628}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. {ECO:0000305}.
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DR EMBL; AF008220; AAC00384.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15007.1; -; Genomic_DNA.
DR PIR; D69585; D69585.
DR RefSeq; NP_390907.1; NC_000964.3.
DR RefSeq; WP_004398630.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; O34518; -.
DR SMR; O34518; -.
DR STRING; 224308.BSU30290; -.
DR PaxDb; O34518; -.
DR EnsemblBacteria; CAB15007; CAB15007; BSU_30290.
DR GeneID; 935927; -.
DR KEGG; bsu:BSU30290; -.
DR PATRIC; fig|224308.179.peg.3285; -.
DR eggNOG; COG0395; Bacteria.
DR InParanoid; O34518; -.
DR OMA; LQRYYMR; -.
DR PhylomeDB; O34518; -.
DR BioCyc; BSUB:BSU30290-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015774; P:polysaccharide transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Polysaccharide transport; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..276
FT /note="Melibiose/raffinose/stachyose import permease
FT protein MelC"
FT /id="PRO_0000387964"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 69..261
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 276 AA; 30375 MW; AC3B77CEA23C8DC8 CRC64;
MRAARTKSMR IITLLAAIVA CAHFIPFYIL LTTSLKAKGD YSSKWIFPAD ISFHNFSEAW
ERASLGNSFI NTMIITGFSA LLLIIFGSLA AYPLARRETK LNKAVFALLI SIMIIPPLTS
MVPLYRMVVD AGMVNTHAIA IFINTAAYMP LTVFLYSGFI RSTIPKELVE AARIDGAGML
KIFFTIVFPL LKPITATICI ISCVFIWNDY QFAIFFLQDQ KVQTLTVAMA GFFGENANNL
HLVAAAALMA MLPMVVLFLA LQKYFIAGLS SGAVKG
