ID   MELR_BACSU              Reviewed;         344 AA.
AC   O34829; Q795R0;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=HTH-type transcriptional repressor MelR {ECO:0000305};
GN   Name=melR {ECO:0000303|PubMed:31138628}; Synonyms=msmR;
GN   OrderedLocusNames=BSU30260;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA   Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT   "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT   200 kb rrnB-dnaB region.";
RL   Microbiology 143:3431-3441(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, AND INDUCTION.
RC   STRAIN=168 / KM0;
RX   PubMed=31138628; DOI=10.1128/jb.00109-19;
RA   Morabbi Heravi K., Watzlawick H., Altenbuchner J.;
RT   "The melREDCA operon encodes a utilization system for the raffinose family
RT   of oligosaccharides in Bacillus subtilis.";
RL   J. Bacteriol. 201:E00109-E00109(2019).
CC   -!- FUNCTION: Represses the melibiose operon melREDCA in the absence of
CC       melibiose or raffinose. Binds to two binding sites at the promoter
CC       region of the operon. {ECO:0000269|PubMed:31138628}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:31138628}.
CC   -!- INDUCTION: Negatively autoregulated. Induced by melibiose and
CC       raffinose. {ECO:0000269|PubMed:31138628}.
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DR   EMBL; AF008220; AAC00387.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15004.1; -; Genomic_DNA.
DR   PIR; A69661; A69661.
DR   RefSeq; NP_390904.1; NC_000964.3.
DR   RefSeq; WP_004398480.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; O34829; -.
DR   SMR; O34829; -.
DR   STRING; 224308.BSU30260; -.
DR   jPOST; O34829; -.
DR   PaxDb; O34829; -.
DR   PRIDE; O34829; -.
DR   EnsemblBacteria; CAB15004; CAB15004; BSU_30260.
DR   GeneID; 937257; -.
DR   KEGG; bsu:BSU30260; -.
DR   PATRIC; fig|224308.179.peg.3282; -.
DR   eggNOG; COG1609; Bacteria.
DR   InParanoid; O34829; -.
DR   OMA; VYTEFMG; -.
DR   PhylomeDB; O34829; -.
DR   BioCyc; BSUB:BSU30260-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   CDD; cd01392; HTH_LacI; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR000843; HTH_LacI.
DR   InterPro; IPR046335; LacI/GalR-like_sensor.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   Pfam; PF00356; LacI; 1.
DR   Pfam; PF13377; Peripla_BP_3; 1.
DR   SMART; SM00354; HTH_LACI; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   PROSITE; PS00356; HTH_LACI_1; 1.
DR   PROSITE; PS50932; HTH_LACI_2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..344
FT                   /note="HTH-type transcriptional repressor MelR"
FT                   /id="PRO_0000360707"
FT   DOMAIN          2..58
FT                   /note="HTH lacI-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT   DNA_BIND        4..23
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
SQ   SEQUENCE   344 AA;  38399 MW;  0C9C394F3FAAC5CE CRC64;
     MVRIKDIALK AKVSSATVSR ILNEDESLSV AGETRQRVIN IAEELGYQTV AKRRKSRGQK
     QRAQPLIGVL SCLSPDQERQ DPYFSSIRKG IEKECFEQEI FITNSIHLGS FQEHIFRELD
     GVIVIGRVHD EAVKHISGRL EHAVFINHSP DPQAYDSIGI DFESASRQAI DHLFDLGYKR
     LGYIGGQEKE HTLKDGQSIR RTIEDKRLTA FLESAAPQPE HVLIGEYSMR EGYRLMKKAI
     DQGHLPEAFF IASDSMAIGA LKALQEAGLQ VPRDTAIVSF NGIEEAEFAS TPLTTVKVYT
     EEMGRTGVKL LLDRLNGRTL PQHVTLPTTL IVRQSCGCTA KEVT