MELR_ECOLI
ID MELR_ECOLI Reviewed; 302 AA.
AC P0ACH8; P10411; Q2M6I6;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Melibiose operon regulatory protein;
GN Name=melR; OrderedLocusNames=b4118, JW4079;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2830169; DOI=10.1016/0378-1119(87)90333-7;
RA Webster C., Kempsell K., Booth I., Busby S.;
RT "Organisation of the regulatory region of the Escherichia coli melibiose
RT operon.";
RL Gene 59:253-263(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION.
RX PubMed=2684786; DOI=10.1016/0378-1119(89)90106-6;
RA Webster C., Gardner L., Busby S.;
RT "The Escherichia coli melR gene encodes a DNA-binding protein with affinity
RT for specific sequences located in the melibiose-operon regulatory region.";
RL Gene 83:207-213(1989).
RN [6]
RP MUTAGENESIS.
RX PubMed=1445207; DOI=10.1042/bj2870493;
RA Caswell R., Williams J., Lyddiatt A., Busby S.;
RT "Overexpression, purification and characterization of the Escherichia coli
RT MelR transcription activator protein.";
RL Biochem. J. 287:493-499(1992).
RN [7]
RP CHARACTERIZATION.
RX PubMed=9108148; DOI=10.1093/nar/25.9.1685;
RA Bourgerie S.J., Michan C.M., Thomas M.S., Busby S.J.W., Hyde E.I.;
RT "DNA binding and DNA bending by the MelR transcription activator protein
RT from Escherichia coli.";
RL Nucleic Acids Res. 25:1685-1693(1997).
CC -!- FUNCTION: Transcription activator for the expression of the melAB
CC operon. MelR binds at two sites located upstream of the melAB
CC transcription site. {ECO:0000269|PubMed:2684786}.
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DR EMBL; M18425; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U14003; AAA97018.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77079.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78120.1; -; Genomic_DNA.
DR PIR; A29625; RGECMB.
DR RefSeq; NP_418542.1; NC_000913.3.
DR RefSeq; WP_001090758.1; NZ_STEB01000014.1.
DR AlphaFoldDB; P0ACH8; -.
DR SMR; P0ACH8; -.
DR BioGRID; 4262686; 12.
DR BioGRID; 852929; 5.
DR DIP; DIP-10181N; -.
DR IntAct; P0ACH8; 15.
DR STRING; 511145.b4118; -.
DR PaxDb; P0ACH8; -.
DR PRIDE; P0ACH8; -.
DR EnsemblBacteria; AAC77079; AAC77079; b4118.
DR EnsemblBacteria; BAE78120; BAE78120; BAE78120.
DR GeneID; 66671971; -.
DR GeneID; 948637; -.
DR KEGG; ecj:JW4079; -.
DR KEGG; eco:b4118; -.
DR PATRIC; fig|1411691.4.peg.2582; -.
DR EchoBASE; EB1212; -.
DR eggNOG; COG2169; Bacteria.
DR HOGENOM; CLU_000445_88_9_6; -.
DR InParanoid; P0ACH8; -.
DR OMA; MCLFWGG; -.
DR PhylomeDB; P0ACH8; -.
DR BioCyc; EcoCyc:PD00208; -.
DR PRO; PR:P0ACH8; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:EcoCyc.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR InterPro; IPR008917; TF_DNA-bd_sf.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47454; SSF47454; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..302
FT /note="Melibiose operon regulatory protein"
FT /id="PRO_0000194537"
FT DOMAIN 194..292
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 211..232
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 259..282
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT MUTAGEN 222
FT /note="N->A: No effect."
FT /evidence="ECO:0000269|PubMed:1445207"
FT MUTAGEN 269
FT /note="R->G: No effect."
FT /evidence="ECO:0000269|PubMed:1445207"
FT MUTAGEN 271
FT /note="S->A: 50% reduced activity."
FT /evidence="ECO:0000269|PubMed:1445207"
FT MUTAGEN 272
FT /note="S->N: 75% reduced activity."
FT /evidence="ECO:0000269|PubMed:1445207"
SQ SEQUENCE 302 AA; 34928 MW; 2D9BC3069AB05029 CRC64;
MNTDTFMCSS DEKQTRSPLS LYSEYQRMEI EFRAPHIMPT SHWHGQVEVN VPFDGDVEYL
INNEKVNINQ GHITLFWACT PHQLTDTGTC QSMAIFNLPM HLFLSWPLDK DLINHVTHGM
VIKSLATQQL SPFEVRRWQQ ELNSPNEQIR QLAIDEIGLM LKRFSLSGWE PILVNKTSRT
HKNSVSRHAQ FYVSQMLGFI AENYDQALTI NDVAEHVKLN ANYAMGIFQR VMQLTMKQYI
TAMRINHVRA LLSDTDKSIL DIALTAGFRS SSRFYSTFGK YVGMSPQQYR KLSQQRRQTF
PG
