MELT_DANRE
ID MELT_DANRE Reviewed; 813 AA.
AC A2BID5; A4IG20; Q8JIG6;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Ventricular zone-expressed PH domain-containing protein;
DE AltName: Full=Protein melted homolog;
GN Name=veph; ORFNames=si:ch211-283j23.5;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=15388229; DOI=10.1016/j.biochi.2004.07.010;
RA Muto E., Tabata Y., Taneda T., Aoki Y., Muto A., Arai K., Watanabe S.;
RT "Identification and characterization of Veph, a novel gene encoding a PH
RT domain-containing protein expressed in the developing central nervous
RT system of vertebrates.";
RL Biochimie 86:523-531(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Ovary;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A2BID5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2BID5-2; Sequence=VSP_027435, VSP_027436;
CC -!- TISSUE SPECIFICITY: Expressed at high levels in kidney, and at low
CC levels in eye, brain, gill and heart. {ECO:0000269|PubMed:15388229}.
CC -!- DEVELOPMENTAL STAGE: Expressed in diencephalon, mesencephalon, anterior
CC hindbrain and otic vesicles at 24 hpf. {ECO:0000269|PubMed:15388229}.
CC -!- DOMAIN: The PH domain is required for membrane targeting.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MELT/VEPH family. {ECO:0000305}.
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DR EMBL; AB085188; BAC02921.1; -; mRNA.
DR EMBL; BX901975; CAM14213.1; -; Genomic_DNA.
DR EMBL; BX255879; CAM14213.1; JOINED; Genomic_DNA.
DR EMBL; BX255879; CAM56445.1; -; Genomic_DNA.
DR EMBL; BX901975; CAM56445.1; JOINED; Genomic_DNA.
DR EMBL; BX255887; CAN88285.1; -; Genomic_DNA.
DR EMBL; CR936375; CAN88285.1; JOINED; Genomic_DNA.
DR EMBL; CU076107; CAN88285.1; JOINED; Genomic_DNA.
DR EMBL; CU076107; CAN88557.1; -; Genomic_DNA.
DR EMBL; BX255887; CAN88557.1; JOINED; Genomic_DNA.
DR EMBL; CR936375; CAN88557.1; JOINED; Genomic_DNA.
DR EMBL; CR936375; CAN88666.1; -; Genomic_DNA.
DR EMBL; BX255887; CAN88666.1; JOINED; Genomic_DNA.
DR EMBL; CU076107; CAN88666.1; JOINED; Genomic_DNA.
DR EMBL; BC134890; AAI34891.1; -; mRNA.
DR RefSeq; NP_997996.1; NM_212831.1.
DR AlphaFoldDB; A2BID5; -.
DR STRING; 7955.ENSDARP00000048984; -.
DR PaxDb; A2BID5; -.
DR GeneID; 405756; -.
DR KEGG; dre:405756; -.
DR CTD; 79674; -.
DR ZFIN; ZDB-GENE-041014-375; veph1.
DR eggNOG; KOG3723; Eukaryota.
DR InParanoid; A2BID5; -.
DR OrthoDB; 139521at2759; -.
DR PhylomeDB; A2BID5; -.
DR TreeFam; TF314736; -.
DR PRO; PR:A2BID5; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0010314; F:phosphatidylinositol-5-phosphate binding; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; IMP:ZFIN.
DR GO; GO:0042471; P:ear morphogenesis; IMP:ZFIN.
DR GO; GO:0009966; P:regulation of signal transduction; IBA:GO_Central.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR039888; Melted-like.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR21630; PTHR21630; 1.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome.
FT CHAIN 1..813
FT /note="Ventricular zone-expressed PH domain-containing
FT protein"
FT /id="PRO_0000297958"
FT DOMAIN 696..799
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 414..445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 484..522
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 484..511
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 450..451
FT /note="SK -> WD (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_027435"
FT VAR_SEQ 452..813
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_027436"
FT CONFLICT 399
FT /note="L -> Q (in Ref. 1; BAC02921 and 3; AAI34891)"
FT /evidence="ECO:0000305"
FT CONFLICT 608
FT /note="E -> A (in Ref. 1; BAC02921)"
FT /evidence="ECO:0000305"
FT CONFLICT 615
FT /note="R -> Q (in Ref. 1; BAC02921)"
FT /evidence="ECO:0000305"
FT CONFLICT 661
FT /note="E -> D (in Ref. 1; BAC02921)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 813 AA; 91209 MW; 6591DE92600CD08D CRC64;
MHQLFRLVLG QRDLSRAGDL FSLDDSEIED CLSQALEEIK VISCSPDYLT NDNDQAVVEI
CITRITTAIR ETGSIERHGA ALVSLWESCL EHNLQPQGKD EDTPHAKIAS DITSCILQNY
NRPPVMALAV PVAVRFLQQG NQELSRNMSS YLSLAAIAKA ELLAQHTEAI TCSVLAGNTM
LLRVLPSVYP RQPEPIHQHV RELTAMMPRL DQTGRQHLLR LLQMISDLHP LVLSSLVPLL
IGFLGDSDLG DSALGVLVNV AQSSPVSLAP FLSTLRNVGQ KNPWLLGHIA KIHRAVGLTS
EIQARGALQY LVSLLASMEH SAHHTLLLEI RCLTDRYTSI LGGGGKDIYR MSNSFSAIAR
LLGRKLEADG AVSRGEEKVQ DLPRPHEAES GSEMFAENLH LQVKIQAFEE KIGDEEVESE
VDGASPGPQR RHSLGQAARD ERREMRFNRS KSLALHAVRT RSINSDSSPE GEGLDLNAEL
NVCEAETSSV SSTPHNHTEL TETQPDTSPN TEGAKVEDGD PRERDRLWIH LRDNMEEIKS
FCAEMIKQIP VPEHCVIEDS HRGCVAKLSF SCPLKGHYCL YGKSSFSLCS SQPHLWIQIM
LLALQSKESE ALCCRDECVL QLRKLWEKTQ LTGAHSFELA MTQTTVPHKK DVDSLQMYLE
EVRFFDLFSY SEDKGGWLCF MCSNPEKATV VNQDGQPLIE GKLKEKQVRW KFIKRWKTRY
FTLAGNQLLF RRGKSKDELD NNTIELSKVQ SVKVVAKKRR DRGLPRAFEI FTDSKTYVLK
AQDEKHAEEW LQCINVAVAQ AKERENREAT TYL
