ID   MEMP_CUPNH              Reviewed;         513 AA.
AC   Q07252; Q0KDV6;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Membrane protein;
GN   OrderedLocusNames=H16_A0667;
OS   Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS   / H16 / Stanier 337) (Ralstonia eutropha).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8405966; DOI=10.1111/j.1574-6968.1993.tb06453.x;
RA   Jendrossek D., Kratzin H.D., Steinbuechel A.;
RT   "The Alcaligenes eutrophus ldh structural gene encodes a novel type of
RT   lactate dehydrogenase.";
RL   FEMS Microbiol. Lett. 112:229-235(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA   Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA   Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT   eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC       DIT1 subfamily. {ECO:0000305}.
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DR   EMBL; Z22737; CAA80431.1; -; Genomic_DNA.
DR   EMBL; AM260479; CAJ91815.1; -; Genomic_DNA.
DR   PIR; I39534; I39534.
DR   AlphaFoldDB; Q07252; -.
DR   SMR; Q07252; -.
DR   STRING; 381666.H16_A0667; -.
DR   EnsemblBacteria; CAJ91815; CAJ91815; H16_A0667.
DR   KEGG; reh:H16_A0667; -.
DR   eggNOG; COG0471; Bacteria.
DR   HOGENOM; CLU_005170_0_1_4; -.
DR   OMA; NFSICLM; -.
DR   Proteomes; UP000008210; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR030676; CitT-rel.
DR   InterPro; IPR001898; SLC13A/DASS.
DR   Pfam; PF00939; Na_sulph_symp; 1.
DR   PIRSF; PIRSF002457; DASS; 1.
DR   TIGRFAMs; TIGR00785; dass; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..513
FT                   /note="Membrane protein"
FT                   /id="PRO_0000172520"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        208..228
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        309..329
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        360..380
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        400..420
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        422..442
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        447..467
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        487..507
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          9..11
FT                   /note="1"
FT   REPEAT          12..14
FT                   /note="2"
FT   REPEAT          15..17
FT                   /note="3"
FT   REPEAT          18..20
FT                   /note="4"
FT   REGION          9..20
FT                   /note="4 X 3 AA tandem repeats of P-S-A"
FT   CONFLICT        74
FT                   /note="V -> L (in Ref. 1; CAA80431)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   513 AA;  53620 MW;  F6576901BEE7C64E CRC64;
     MTDTSASPPS APSAPSAPSA AAAQAVVPAP KLTLHWGLFA AVAALLVVLA IPQPEGLTVA
     GQRMLAILAF AIVVWITEAV SYETSAIMIT SLMAGLIGFA PTVNDPSVQY GTSKALGMAL
     AGFSNTALAL VAAALFISAA MTVTGLDRRI ALVTLSAIGT STRRILIGTI AVTIALSLVV
     PSATARSACV VPIMMGVIAA FGVDKKSNIA AGIMITVAQA TSIWNVGIQT AAAQNLLTVG
     FMDKLLGERI TWLQWLIAGA PWAIAMSVVL YFLVRLLLPA ETDAIPGGKE AVQRELSALG
     PMSAPQKRLA AVSLGLLLFW ATEGKLHSFD TATVTFVGLV ILMMPRIGVM DWKTMQQRTP
     WGTLIVFGVG ISLGTALLST NAGQWLGQFV VTHSGLAAHG ALLVFAILSA FLILIHLGFA
     SATALTAALL PILIAVLQTL PGDINRVGMT MLLGFTVSFG FILPINAPQN MVCLGTETFN
     GRQFARIGIP VTIIGYAMML LFAATYWRWL GWV