ID   MEN1_EUPNO              Reviewed;          52 AA.
AC   P83441;
DT   27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Mating pheromone En-1;
OS   Euplotes nobilii (Ciliate).
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC   Hypotrichia; Euplotida; Euplotidae; Euplotes.
OX   NCBI_TaxID=184062 {ECO:0000305};
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   STRAIN=AC-1;
RX   PubMed=12667606; DOI=10.1016/s0304-4165(03)00011-4;
RA   Alimenti C., Ortenzi C., Carratore V., Luporini P.;
RT   "Structural characterization of En-1, a cold-adapted protein pheromone
RT   isolated from the Antarctic ciliate Euplotes nobilii.";
RL   Biochim. Biophys. Acta 1621:17-21(2003).
RN   [2]
RP   STRUCTURE BY NMR, AND DISULFIDE BONDS.
RX   PubMed=19621350; DOI=10.1002/iub.228;
RA   Alimenti C., Vallesi A., Pedrini B., Wuthrich K., Luporini P.;
RT   "Molecular cold-adaptation: comparative analysis of two homologous families
RT   of psychrophilic and mesophilic signal proteins of the protozoan ciliate,
RT   Euplotes.";
RL   IUBMB Life 61:838-845(2009).
CC   -!- FUNCTION: Mating ciliate pheromones (or gamones) are diffusible
CC       extracellular communication signals that distinguish different
CC       intraspecific classes of cells commonly referred to as 'mating types'.
CC       They prepare the latter for conjugation by changing their cell surface
CC       properties. {ECO:0000269|PubMed:12667606}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12667606,
CC       ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=5617.6; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:12667606};
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DR   PDB; 2KC6; NMR; -; A=1-52.
DR   PDB; 2NSV; NMR; -; A=1-52.
DR   PDBsum; 2KC6; -.
DR   PDBsum; 2NSV; -.
DR   AlphaFoldDB; P83441; -.
DR   BMRB; P83441; -.
DR   SMR; P83441; -.
DR   EvolutionaryTrace; P83441; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005186; F:pheromone activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Pheromone;
KW   Secreted.
FT   CHAIN           1..52
FT                   /note="Mating pheromone En-1"
FT                   /id="PRO_0000186199"
FT   DISULFID        11..37
FT                   /evidence="ECO:0000269|PubMed:19621350"
FT   DISULFID        23..33
FT                   /evidence="ECO:0000269|PubMed:19621350"
FT   DISULFID        30..52
FT                   /evidence="ECO:0000269|PubMed:19621350"
FT   DISULFID        34..46
FT                   /evidence="ECO:0000269|PubMed:19621350"
FT   TURN            2..5
FT                   /evidence="ECO:0007829|PDB:2KC6"
FT   TURN            8..10
FT                   /evidence="ECO:0007829|PDB:2KC6"
FT   HELIX           16..23
FT                   /evidence="ECO:0007829|PDB:2KC6"
FT   STRAND          25..28
FT                   /evidence="ECO:0007829|PDB:2KC6"
FT   HELIX           31..36
FT                   /evidence="ECO:0007829|PDB:2KC6"
FT   HELIX           40..47
FT                   /evidence="ECO:0007829|PDB:2KC6"
SQ   SEQUENCE   52 AA;  5630 MW;  61FC858BE81C3BC7 CRC64;
     NPEDWFTPDT CAYGDSNTAW TTCTTPGQTC YTCCSSCFDV VGEQACQMSA QC