ID   MEN2_EUPNO              Reviewed;          60 AA.
AC   P83235;
DT   20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Mating pheromone En-2;
OS   Euplotes nobilii (Ciliate).
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC   Hypotrichia; Euplotida; Euplotidae; Euplotes.
OX   NCBI_TaxID=184062;
RN   [1]
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   STRAIN=AC-1;
RX   PubMed=11943175; DOI=10.1016/s0014-5793(02)02393-1;
RA   Alimenti C., Ortenzi C., Carratore V., Luporini P.;
RT   "Structural characterization of a protein pheromone from a cold-adapted
RT   (Antarctic) single-cell eukaryote, the ciliate Euplotes nobilii.";
RL   FEBS Lett. 514:329-332(2002).
RN   [2]
RP   STRUCTURE BY NMR, AND DISULFIDE BONDS.
RX   PubMed=19621350; DOI=10.1002/iub.228;
RA   Alimenti C., Vallesi A., Pedrini B., Wuthrich K., Luporini P.;
RT   "Molecular cold-adaptation: comparative analysis of two homologous families
RT   of psychrophilic and mesophilic signal proteins of the protozoan ciliate,
RT   Euplotes.";
RL   IUBMB Life 61:838-845(2009).
CC   -!- FUNCTION: Mating ciliate pheromones (or gamones) are diffusible
CC       extracellular communication signals that distinguish different
CC       intraspecific classes of cells commonly referred to as 'mating types'.
CC       They prepare the latter for conjugation by changing their cell surface
CC       properties.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11943175}.
CC   -!- MASS SPECTROMETRY: Mass=6298.92; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:11943175};
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DR   PDB; 2NSW; NMR; -; A=1-60.
DR   PDBsum; 2NSW; -.
DR   AlphaFoldDB; P83235; -.
DR   BMRB; P83235; -.
DR   SMR; P83235; -.
DR   EvolutionaryTrace; P83235; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005186; F:pheromone activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Pheromone;
KW   Secreted.
FT   CHAIN           1..60
FT                   /note="Mating pheromone En-2"
FT                   /id="PRO_0000186200"
FT   DISULFID        11..39
FT                   /evidence="ECO:0000269|PubMed:19621350"
FT   DISULFID        24..35
FT                   /evidence="ECO:0000269|PubMed:19621350"
FT   DISULFID        31..57
FT                   /evidence="ECO:0000269|PubMed:19621350"
FT   DISULFID        36..48
FT                   /evidence="ECO:0000269|PubMed:19621350"
FT   HELIX           3..5
FT                   /evidence="ECO:0007829|PDB:2NSW"
FT   TURN            8..10
FT                   /evidence="ECO:0007829|PDB:2NSW"
FT   HELIX           17..24
FT                   /evidence="ECO:0007829|PDB:2NSW"
FT   STRAND          27..30
FT                   /evidence="ECO:0007829|PDB:2NSW"
FT   HELIX           35..37
FT                   /evidence="ECO:0007829|PDB:2NSW"
FT   TURN            42..44
FT                   /evidence="ECO:0007829|PDB:2NSW"
FT   HELIX           45..48
FT                   /evidence="ECO:0007829|PDB:2NSW"
FT   STRAND          54..58
FT                   /evidence="ECO:0007829|PDB:2NSW"
SQ   SEQUENCE   60 AA;  6304 MW;  6EDF181F712E29CD CRC64;
     DIEDFYTSET CPYKNDSQLA WDTCSGGTGN CGTVCCGQCF SFPVSQSCAG MADSNDCPNA