ARHGJ_MOUSE
ID ARHGJ_MOUSE Reviewed; 802 AA.
AC Q8BWA8; Q6PAC2;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Rho guanine nucleotide exchange factor 19;
DE AltName: Full=Ephexin-2;
DE AltName: Full=Weakly similar to Rho GEF 5;
GN Name=Arhgef19; Synonyms=Wgef;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=129; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 522-531, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15485661; DOI=10.1016/j.bbrc.2004.09.153;
RA Wang Y., Suzuki H., Yokoo T., Tada-Iida K., Kihara R., Miura M.,
RA Watanabe K., Sone H., Shimano H., Toyoshima H., Yamada N.;
RT "WGEF is a novel RhoGEF expressed in intestine, liver, heart, and kidney.";
RL Biochem. Biophys. Res. Commun. 324:1053-1058(2004).
RN [6]
RP IDENTIFICATION.
RX PubMed=15848799; DOI=10.1016/j.neuron.2005.01.030;
RA Sahin M., Greer P.L., Lin M.Z., Poucher H., Eberhart J., Schmidt S.,
RA Wright T.M., Shamah S.M., O'connell S., Cowan C.W., Hu L., Goldberg J.L.,
RA Debant A., Corfas G., Krull C.E., Greenberg M.E.;
RT "Eph-dependent tyrosine phosphorylation of ephexin1 modulates growth cone
RT collapse.";
RL Neuron 46:191-204(2005).
CC -!- FUNCTION: Acts as guanine nucleotide exchange factor (GEF) for RhoA
CC GTPase. {ECO:0000269|PubMed:15485661}.
CC -!- TISSUE SPECIFICITY: Highly expressed in intestine, and at lower levels
CC in liver, heart and kidney. {ECO:0000269|PubMed:15485661}.
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DR EMBL; AK053075; BAC35256.1; -; mRNA.
DR EMBL; AL607087; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC060376; AAH60376.1; -; mRNA.
DR CCDS; CCDS18868.1; -.
DR RefSeq; NP_766108.1; NM_172520.2.
DR RefSeq; XP_006538767.1; XM_006538704.2.
DR RefSeq; XP_006538768.1; XM_006538705.2.
DR RefSeq; XP_006538769.1; XM_006538706.3.
DR RefSeq; XP_006538770.1; XM_006538707.3.
DR RefSeq; XP_006538771.1; XM_006538708.3.
DR RefSeq; XP_006538772.1; XM_006538709.3.
DR RefSeq; XP_011248527.1; XM_011250225.1.
DR RefSeq; XP_017175587.1; XM_017320098.1.
DR AlphaFoldDB; Q8BWA8; -.
DR SMR; Q8BWA8; -.
DR BioGRID; 229460; 5.
DR STRING; 10090.ENSMUSP00000006618; -.
DR iPTMnet; Q8BWA8; -.
DR PhosphoSitePlus; Q8BWA8; -.
DR jPOST; Q8BWA8; -.
DR MaxQB; Q8BWA8; -.
DR PaxDb; Q8BWA8; -.
DR PRIDE; Q8BWA8; -.
DR ProteomicsDB; 265091; -.
DR Antibodypedia; 46665; 84 antibodies from 27 providers.
DR DNASU; 213649; -.
DR Ensembl; ENSMUST00000006618; ENSMUSP00000006618; ENSMUSG00000028919.
DR GeneID; 213649; -.
DR KEGG; mmu:213649; -.
DR UCSC; uc008vob.1; mouse.
DR CTD; 128272; -.
DR MGI; MGI:1925912; Arhgef19.
DR VEuPathDB; HostDB:ENSMUSG00000028919; -.
DR eggNOG; KOG3523; Eukaryota.
DR GeneTree; ENSGT01030000234571; -.
DR HOGENOM; CLU_012820_1_0_1; -.
DR InParanoid; Q8BWA8; -.
DR OMA; PSMWIPG; -.
DR OrthoDB; 1176939at2759; -.
DR PhylomeDB; Q8BWA8; -.
DR TreeFam; TF316357; -.
DR Reactome; R-MMU-193648; NRAGE signals death through JNK.
DR Reactome; R-MMU-416482; G alpha (12/13) signalling events.
DR Reactome; R-MMU-8980692; RHOA GTPase cycle.
DR Reactome; R-MMU-9013148; CDC42 GTPase cycle.
DR Reactome; R-MMU-9013149; RAC1 GTPase cycle.
DR BioGRID-ORCS; 213649; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Arhgef19; mouse.
DR PRO; PR:Q8BWA8; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8BWA8; protein.
DR Bgee; ENSMUSG00000028919; Expressed in dorsal pancreas and 173 other tissues.
DR ExpressionAtlas; Q8BWA8; baseline and differential.
DR Genevisible; Q8BWA8; MM.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IGI:MGI.
DR GO; GO:0032956; P:regulation of actin cytoskeleton organization; ISO:MGI.
DR GO; GO:0042060; P:wound healing; IMP:MGI.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.20.900.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR Pfam; PF00621; RhoGEF; 1.
DR Pfam; PF00018; SH3_1; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SMART; SM00326; SH3; 1.
DR SUPFAM; SSF48065; SSF48065; 1.
DR SUPFAM; SSF50044; SSF50044; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50002; SH3; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; GTPase activation;
KW Guanine-nucleotide releasing factor; Reference proteome; SH3 domain.
FT CHAIN 1..802
FT /note="Rho guanine nucleotide exchange factor 19"
FT /id="PRO_0000285132"
FT DOMAIN 376..560
FT /note="DH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
FT DOMAIN 592..704
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 715..776
FT /note="SH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 77..136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 173..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 218..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 307..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..129
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 272..286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 492
FT /note="G -> S (in Ref. 3; AAH60376)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 802 AA; 88851 MW; 9066207998966E77 CRC64;
MDCGPPATLQ PHLAGPPGTA RRPVAVCQQE SLSFVELPPS RLCVDLFPIA PEELQAPGSR
WSLATPAPLQ GLLWPTSPGG PDTHIASTSG GMRPGRAGSW PHCPGAQPPT LVEPWSSQHM
QPQRRASHSG SEKSAWRKMQ VYHNEEATGT ETPTGLLETG QAAQEQALCA LEPGPQELSG
NARGGLEAPE RRRFSASELM TRLHSSLRLG RSTATRVLTS GSGAGAIREG KLPVRESRRA
ETRADSVSLP GPVDLNEVHG GLVEPRLDTQ DKPTQDPSSA TERRQSRFLL NSVLYQEYSD
VASARELRRQ QREEEGPGDG AEGAEEGPGP PRANLSPSSS FRAQRSTRGS TFSLWQDIPD
VRGSGVLATL SLRDCKLQEA KFELITSEAS YIHSLSVAVG HFLGSVELSE CLGTQDKQWL
FSKLPEVKST SERFLHDLEQ RLEADVLRFS VCDVVLHHCP AFRRVYLPYV TNQAYQERTY
QRLLLENPKF PGILARLEES PVCQRLPLTS FLILPFQRVT RLKMLVENIL KRTAPGSQDE
DMATKAFSAL KELVQECNAS VQSMKRTEEL IHLSKKIHFE GKIFPLISQA RWLVRHGELV
ELAPLPAAPP AKLKLSSKAV YLHLFNDCLL LSRRKELGKF AVFVHANMAE LQVRDLSLKL
QGIPGHVFLL RLLHGQRARH QLLLRARTES EKQRWISALR PSSPQEDKEI TCDGEDRPQV
QCVRTYKALQ PDELTLEKTD ILAVKTRTSD GWLEGVRLAD GEKGWVPQAH VVEISSLSAR
LRNLREYKRV SNASSKLGDP PA
