ID   ARHL1_XENLA             Reviewed;         354 AA.
AC   Q6AZR2;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=Inactive ADP-ribosyltransferase arh2 {ECO:0000305};
DE   AltName: Full=ADP-ribosylhydrolase-like protein 1 {ECO:0000305};
DE   AltName: Full=[Protein ADP-ribosylarginine] hydrolase-like protein 1;
GN   Name=adprhl1; Synonyms=arh2;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP   DISRUPTION PHENOTYPE, AND 3D-STRUCTURE MODELING.
RX   PubMed=27217161; DOI=10.1016/j.ydbio.2016.05.006;
RA   Smith S.J., Towers N., Saldanha J.W., Shang C.A., Mahmood S.R.,
RA   Taylor W.R., Mohun T.J.;
RT   "The cardiac-restricted protein ADP-ribosylhydrolase-like 1 is essential
RT   for heart chamber outgrowth and acts on muscle actin filament assembly.";
RL   Dev. Biol. 416:373-388(2016).
CC   -!- FUNCTION: Required for myofibril assembly and outgrowth of the cardiac
CC       chambers in the developing heart (PubMed:27217161). Appears to be
CC       catalytically inactive, showing no activity against O-acetyl-ADP-ribose
CC       (By similarity). {ECO:0000250|UniProtKB:Q8BGK2,
CC       ECO:0000269|PubMed:27217161}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, myofibril, sarcomere
CC       {ECO:0000269|PubMed:27217161}.
CC   -!- TISSUE SPECIFICITY: Expressed in heart (at protein level)
CC       (PubMed:27217161). A short form is detected in both heart and tadpole
CC       tail (at protein level) (PubMed:27217161).
CC       {ECO:0000269|PubMed:27217161}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in embryos, juveniles and adults. Onset
CC       of embryonic expression coincides with early myocardial differentiation
CC       (PubMed:27217161). Expression is detected in cardiac tissue from stage
CC       29 with the greatest concentration found in regions of actively growing
CC       chamber myocardium (PubMed:27217161). At stage 40, highly expressed in
CC       the left and right lateral sides marking the growing atrial chambers
CC       with lower levels in the dorsal atrial roof (PubMed:27217161).
CC       {ECO:0000269|PubMed:27217161}.
CC   -!- DISRUPTION PHENOTYPE: Morpholino knockdown results in defective
CC       embryonic cardiogenesis with hearts forming small ventricles that do
CC       not beat (PubMed:27217161). Defective endocardial maturation with
CC       endocardial cells showing a rounded morphology and no clear lumen
CC       forming in the heart (PubMed:27217161). Myofibril assembly is disrupted
CC       in the developing ventricle, preventing ventricle outgrowth
CC       (PubMed:27217161). {ECO:0000269|PubMed:27217161}.
CC   -!- SIMILARITY: Belongs to the ADP-ribosylglycohydrolase family.
CC       {ECO:0000305}.
CC   -!- CAUTION: Although it belongs to the ADP-ribosylglycohydrolase family,
CC       lacks metal-binding and substrate-binding residues, suggesting that it
CC       has no hydrolase activity. {ECO:0000305|PubMed:27217161}.
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DR   EMBL; BC077455; AAH77455.1; -; mRNA.
DR   RefSeq; NP_001086791.1; NM_001093322.1.
DR   AlphaFoldDB; Q6AZR2; -.
DR   SMR; Q6AZR2; -.
DR   DNASU; 446626; -.
DR   GeneID; 446626; -.
DR   KEGG; xla:446626; -.
DR   CTD; 446626; -.
DR   Xenbase; XB-GENE-989263; adprhl1.S.
DR   OrthoDB; 1112828at2759; -.
DR   BRENDA; 3.2.2.19; 6725.
DR   Proteomes; UP000186698; Chromosome 2S.
DR   Bgee; 446626; Expressed in heart and 4 other tissues.
DR   GO; GO:0030017; C:sarcomere; IMP:UniProtKB.
DR   GO; GO:0003875; F:ADP-ribosylarginine hydrolase activity; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0003242; P:cardiac chamber ballooning; IMP:UniProtKB.
DR   GO; GO:0055003; P:cardiac myofibril assembly; IMP:UniProtKB.
DR   GO; GO:0051725; P:protein de-ADP-ribosylation; IEA:InterPro.
DR   Gene3D; 1.10.4080.10; -; 1.
DR   InterPro; IPR012108; ADP-ribosylarg_hydro.
DR   InterPro; IPR005502; Ribosyl_crysJ1.
DR   InterPro; IPR036705; Ribosyl_crysJ1_sf.
DR   Pfam; PF03747; ADP_ribosyl_GH; 1.
DR   PIRSF; PIRSF016939; ADP_ribslarg_hdr; 1.
DR   SUPFAM; SSF101478; SSF101478; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome.
FT   CHAIN           1..354
FT                   /note="Inactive ADP-ribosyltransferase arh2"
FT                   /id="PRO_0000277611"
SQ   SEQUENCE   354 AA;  40205 MW;  EDE57B6C83095DE5 CRC64;
     MEKFKAAMLL AGTGDALGYK NFSWVFCASG VKIQEELKQL GGLENLVLSI DGWPVSNNTL
     MHIATAESLV SDYWSIEDLY RDMVKRYIDV VDKLQGRRPD PATIEGCAHL KPDNYLLAWH
     TPFNEKGSGF GAATKAMCIG MRYWKPGRLE TLIEVSIESG RMTHNHPTGF LGSLCTALFT
     SYAIQEKPLA QWGREMLKLL PMAEEYCKKT IRHMAEYQEH WFYFEAKWQF YLEEREIAEE
     NENKPKFPDK YDAEERDKAY KTWSSEGRGG RRGHDAPMIA YDALLGAGGD WKELCNRAMF
     HGGEGGATGS IAGCLYGLLY GISKVPKSLY QQLEMKERLE NLGEKLYHLA TMEK