MEP1_YEAST
ID MEP1_YEAST Reviewed; 492 AA.
AC P40260; D6VUQ2;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Ammonium transporter MEP1;
GN Name=MEP1; Synonyms=AMT1; OrderedLocusNames=YGR121C; ORFNames=G6331;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Sigma 1278B;
RX PubMed=8062822; DOI=10.1002/j.1460-2075.1994.tb06651.x;
RA Marini A.-M., Vissers S., Urrestarazu A., Andre B.;
RT "Cloning and expression of the MEP1 gene encoding an ammonium transporter
RT in Saccharomyces cerevisiae.";
RL EMBO J. 13:3456-3463(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=9046098;
RX DOI=10.1002/(sici)1097-0061(199702)13:2<171::aid-yea57>3.0.co;2-v;
RA van Dyck L., Tettelin H., Purnelle B., Goffeau A.;
RT "An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown
RT open reading frames, the gene for an Asn synthase, remnants of Ty and three
RT tRNA genes.";
RL Yeast 13:171-176(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP FUNCTION.
RC STRAIN=Sigma 1278B;
RX PubMed=9234685; DOI=10.1128/mcb.17.8.4282;
RA Marini A.-M., Soussi-Boudekou S., Vissers S., Andre B.;
RT "A family of ammonium transporters in Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 17:4282-4293(1997).
RN [6]
RP FUNCTION.
RX PubMed=11486013; DOI=10.1128/mcb.21.17.5733-5741.2001;
RA Soupene E., Ramirez R.M., Kustu S.;
RT "Evidence that fungal MEP proteins mediate diffusion of the uncharged
RT species NH(3) across the cytoplasmic membrane.";
RL Mol. Cell. Biol. 21:5733-5741(2001).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442 AND SER-445, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Transporter for ammonium (both charged and uncharged NH3 and
CC NH4) to use as a nitrogen source. Can also transport methylamine. The
CC affinity of MEP1 is about twenty times lower than that of MEP2. MEP3
CC has the lowest affinity. {ECO:0000269|PubMed:11486013,
CC ECO:0000269|PubMed:9234685}.
CC -!- INTERACTION:
CC P40260; P53390: MEP3; NbExp=3; IntAct=EBI-10714, EBI-10729;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ammonia transporter channel (TC 1.A.11.2)
CC family. {ECO:0000305}.
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DR EMBL; X77608; CAA54699.1; -; Genomic_DNA.
DR EMBL; X83099; CAA58156.1; -; Genomic_DNA.
DR EMBL; Z72906; CAA97132.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08213.1; -; Genomic_DNA.
DR PIR; S46225; S46225.
DR RefSeq; NP_011636.3; NM_001181250.3.
DR AlphaFoldDB; P40260; -.
DR SMR; P40260; -.
DR BioGRID; 33367; 132.
DR DIP; DIP-5530N; -.
DR IntAct; P40260; 14.
DR MINT; P40260; -.
DR STRING; 4932.YGR121C; -.
DR TCDB; 1.A.11.3.1; the ammonium transporter channel (amt) family.
DR iPTMnet; P40260; -.
DR PaxDb; P40260; -.
DR PRIDE; P40260; -.
DR EnsemblFungi; YGR121C_mRNA; YGR121C; YGR121C.
DR GeneID; 853019; -.
DR KEGG; sce:YGR121C; -.
DR SGD; S000003353; MEP1.
DR VEuPathDB; FungiDB:YGR121C; -.
DR eggNOG; KOG0682; Eukaryota.
DR GeneTree; ENSGT00530000064546; -.
DR HOGENOM; CLU_000445_33_0_1; -.
DR InParanoid; P40260; -.
DR OMA; MACWNSN; -.
DR BioCyc; YEAST:G3O-30828-MON; -.
DR PRO; PR:P40260; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P40260; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IDA:SGD.
DR GO; GO:0072488; P:ammonium transmembrane transport; IMP:SGD.
DR GO; GO:0019740; P:nitrogen utilization; IMP:SGD.
DR Gene3D; 1.10.3430.10; -; 1.
DR InterPro; IPR029020; Ammonium/urea_transptr.
DR InterPro; IPR001905; Ammonium_transpt.
DR InterPro; IPR018047; Ammonium_transpt_CS.
DR InterPro; IPR024041; NH4_transpt_AmtB-like_dom.
DR PANTHER; PTHR43029; PTHR43029; 1.
DR Pfam; PF00909; Ammonium_transp; 1.
DR TIGRFAMs; TIGR00836; amt; 1.
DR PROSITE; PS01219; AMMONIUM_TRANSP; 1.
PE 1: Evidence at protein level;
KW Ammonia transport; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..492
FT /note="Ammonium transporter MEP1"
FT /id="PRO_0000139754"
FT TOPO_DOM 1..18
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..49
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 71..109
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..140
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 162..174
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..210
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 232..240
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..268
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 290
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..331
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..373
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..492
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 455..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 455..475
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 442
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 445
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 492 AA; 54202 MW; CC7DF0EB838D0B77 CRC64;
MESRTTGPLT TETYDGPTVA FMILGAALVF FMVPGLGFLY SGLARRKSAL ALIWVVLMAT
LVGILQWYFW GYSLAFSKSA PNNKFIGNLD SFGFRNVYGK KFDEDAYPEL AYATFQMMFS
CVNLSIIAGA TAERGRLLPH MVFLFILATI GYCPVTYWIW SPGGWAYQWG VLDWAGGGNI
EILSAVSGFV YSWFLGKRNE KLLINFRPHN VSLVTLGTSI LWFGWLLFNS ASSLSPNLRS
VYAFMNTCLS AITGGMTWCL LDYRSEKKWS TVGLCSGIIS GLVAATPSSG CITLYGSLIQ
GIVAGVVCNF ATKLKYYAKV DDAMDILAEH GVAGVIGLIF NALFGADWVI GMDGTTEHEG
GWVTHNYKQM YKQIAYIAAS IGYTAAVTAI ICFVLGYIPG MRLRISEEAE EAGMDEDQIG
EFAYDYVEVR RDYYLWGVDE DSQRSDVNHR VNNAHLAAER SSSGTNSSSD GNGEMIQSEK
ILPIHQEDPA NR
