MEP33_SCHPO
ID MEP33_SCHPO Reviewed; 292 AA.
AC Q9USV4; P78845; P78935;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=mRNA export protein 33;
GN Name=mep33; ORFNames=SPBC28F2.02;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RA Kohnosu A., Niwa O., Yano M., Saitoh S., Katayama T., Nagao K.,
RA Yanagida M.;
RT "S.pombe chromosome II cosmid 1228 sequence.";
RL Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-292.
RC STRAIN=PR745;
RX PubMed=9501991; DOI=10.1093/dnares/4.6.363;
RA Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.;
RT "Identification of open reading frames in Schizosaccharomyces pombe
RT cDNAs.";
RL DNA Res. 4:363-369(1997).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15116432; DOI=10.1002/yea.1115;
RA Chen X.Q., Du X., Liu J., Balasubramanian M.K., Balasundaram D.;
RT "Identification of genes encoding putative nucleoporins and transport
RT factors in the fission yeast Schizosaccharomyces pombe: a deletion
RT analysis.";
RL Yeast 21:495-509(2004).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Functions as a component of the nuclear pore complex (NPC).
CC NPC components, collectively referred to as nucleoporins (NUPs), can
CC play the role of both NPC structural components and of docking or
CC interaction partners for transiently associated nuclear transport
CC factors. Active directional transport is assured by both, a Phe-Gly
CC (FG) repeat affinity gradient for these transport factors across the
CC NPC and a transport cofactor concentration gradient across the nuclear
CC envelope. Involved in the export of mRNA from the nucleus to the
CC cytoplasm. May play a role in mitotic spindle formation and/or
CC function. {ECO:0000269|PubMed:15116432}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15116432,
CC ECO:0000269|PubMed:16823372}.
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DR EMBL; D83992; BAA12184.1; -; Genomic_DNA.
DR EMBL; CU329671; CAB57931.1; -; Genomic_DNA.
DR EMBL; D89195; BAA13856.1; -; mRNA.
DR PIR; T40045; T40045.
DR PIR; T42994; T42994.
DR RefSeq; NP_595663.1; NM_001021558.2.
DR AlphaFoldDB; Q9USV4; -.
DR SMR; Q9USV4; -.
DR BioGRID; 276928; 7.
DR STRING; 4896.SPBC28F2.02.1; -.
DR iPTMnet; Q9USV4; -.
DR SwissPalm; Q9USV4; -.
DR MaxQB; Q9USV4; -.
DR PaxDb; Q9USV4; -.
DR PRIDE; Q9USV4; -.
DR EnsemblFungi; SPBC28F2.02.1; SPBC28F2.02.1:pep; SPBC28F2.02.
DR GeneID; 2540400; -.
DR KEGG; spo:SPBC28F2.02; -.
DR PomBase; SPBC28F2.02; mep33.
DR VEuPathDB; FungiDB:SPBC28F2.02; -.
DR eggNOG; KOG1763; Eukaryota.
DR HOGENOM; CLU_042870_1_0_1; -.
DR InParanoid; Q9USV4; -.
DR OMA; LWQCPNG; -.
DR PhylomeDB; Q9USV4; -.
DR PRO; PR:Q9USV4; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0002181; P:cytoplasmic translation; ISO:PomBase.
DR GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:PomBase.
DR InterPro; IPR032378; ZC3H15/TMA46_C.
DR InterPro; IPR000571; Znf_CCCH.
DR Pfam; PF16543; DFRP_C; 1.
DR PROSITE; PS50103; ZF_C3H1; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Metal-binding; mRNA transport; Reference proteome; Transport;
KW Zinc; Zinc-finger.
FT CHAIN 1..292
FT /note="mRNA export protein 33"
FT /id="PRO_0000116773"
FT ZN_FING 134..172
FT /note="C3H1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..26
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..57
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 60..76
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 111..112
FT /note="QK -> PQ (in Ref. 1; BAA12184)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 292 AA; 33282 MW; 8233D2544DC74207 CRC64;
MPPKKAAKGK GDPGKAAKKD PTKKAADATF GLKNKNRSTK VQAKIRQIEQ NAAASGSKDA
KRQEALRKRR EEEKRAAEAA KAEVAALFNA IPKKQTPQNF LTRKEEVKES QKIDLYSDVR
DQQTDLPLEK RPWINTDIVC KFFLEACETG KYGWLWQCPN GNMTCIYKHA LPYGYVLSRD
KKKDDTKEEI SLEAFIEIER HRLGPNLTPV TEENFKKWSD GRRDRILKQA EERRSNRAVG
RSNLSGREYF ESNKDKTHEV VGDEEDWDFS ALRRETEALA KAQDATAPIV SV
