ID   MEP50_PONAB             Reviewed;         342 AA.
AC   Q5RBZ2;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Methylosome protein 50;
DE            Short=MEP-50;
DE   AltName: Full=WD repeat-containing protein 77;
GN   Name=WDR77; Synonyms=MEP50;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Non-catalytic component of the methylosome complex, composed
CC       of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to
CC       dimethylarginines in several spliceosomal Sm proteins and histones.
CC       This modification targets Sm proteins to the survival of motor neurons
CC       (SMN) complex for assembly into small nuclear ribonucleoprotein core
CC       particles. Might play a role in transcription regulation. The
CC       methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2
CC       and PIWIL4), methylation of Piwi proteins being required for the
CC       interaction with Tudor domain-containing proteins and subsequent
CC       localization to the meiotic nuage. {ECO:0000250|UniProtKB:Q99J09,
CC       ECO:0000250|UniProtKB:Q9BQA1}.
CC   -!- SUBUNIT: Component of the methylosome complex composed of PRMT5, WDR77
CC       and CLNS1A (By similarity). Found in a complex composed of PRMT5, WDR77
CC       and RIOK1 (By similarity). RIOK1 and CLNS1A bound directly to PRMT5 at
CC       the same binding site, in a mutually exclusive manner, which allows the
CC       recruitment of distinct methylation substrates, such as nucleolin/NCL
CC       and Sm proteins, respectively (By similarity). Found in a complex with
CC       the component of the methylosome, PRMT5, CLNS1A, WDR77, PRMT1 and ERH.
CC       Directly interacts with PRMT5, as well as with several Sm proteins,
CC       including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Forms a
CC       compact hetero-octamer with PRMT5, decorating the outer surface of a
CC       PRMT5 tetramer. Interacts with SUZ12 and histone H2A/H2AC20, but not
CC       with histones H2B, H3 nor H4. Interacts with CTDP1 and LSM11. Interacts
CC       with APEX1, AR and NKX3-1. Interacts with CHTOP. Interacts with FAM47E.
CC       {ECO:0000250|UniProtKB:Q99J09, ECO:0000250|UniProtKB:Q9BQA1}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9BQA1}. Cytoplasm
CC       {ECO:0000250|UniProtKB:Q9BQA1}.
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DR   EMBL; CR858490; CAH90718.1; -; mRNA.
DR   RefSeq; NP_001125399.1; NM_001131927.1.
DR   AlphaFoldDB; Q5RBZ2; -.
DR   SMR; Q5RBZ2; -.
DR   STRING; 9601.ENSPPYP00000001202; -.
DR   GeneID; 100172304; -.
DR   KEGG; pon:100172304; -.
DR   CTD; 79084; -.
DR   eggNOG; KOG0284; Eukaryota.
DR   InParanoid; Q5RBZ2; -.
DR   OrthoDB; 774546at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0034709; C:methylosome; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0008327; F:methyl-CpG binding; ISS:UniProtKB.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF00400; WD40; 1.
DR   SMART; SM00320; WD40; 6.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 2.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..342
FT                   /note="Methylosome protein 50"
FT                   /id="PRO_0000331608"
FT   REPEAT          22..75
FT                   /note="WD 1"
FT   REPEAT          78..116
FT                   /note="WD 2"
FT   REPEAT          123..162
FT                   /note="WD 3"
FT   REPEAT          165..205
FT                   /note="WD 4"
FT   REPEAT          209..250
FT                   /note="WD 5"
FT   REPEAT          253..293
FT                   /note="WD 6"
FT   REPEAT          295..330
FT                   /note="WD 7"
FT   MOD_RES         5
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9BQA1"
SQ   SEQUENCE   342 AA;  36818 MW;  E7C80B9C755CF460 CRC64;
     MRKETPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG ALLLGASSLS GRCWAGSLWL
     FKDPCAAPNE GFCSAGVQTE AGVADLTWVG ERGILVASDS GAVELWELDE NETLIVSKFC
     KYEHDDIVST VSVLSSGTQA VSGSKDICIK VWDLAQQVVL NSYRAHAAQV TCVAASPHKD
     SVFLSCSEDN RILLWDTRRP KPASQIGCSA PGYLPTSLAW HPQQSEVFVF GDENGTVSLV
     DTKSTSCVLS SAVHSQCVTG LVFSPHSVPF LASLSEDCSL AVLDSSLSEL FRSQAHRDFV
     RDATWSPLNH SLLTTVGWDH QVVHHIVPTE PLPAPGPASV TE