MEPA_STAA8
ID MEPA_STAA8 Reviewed; 451 AA.
AC Q2G140; Q5Y811;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Multidrug export protein MepA;
DE AltName: Full=Staphylococcal virulence regulator protein A;
GN Name=mepA; Synonyms=svrA; OrderedLocusNames=SAOUHSC_00315;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN MULTIDRUG RESISTANCE, AND
RP INDUCTION.
RX PubMed=15855507; DOI=10.1128/aac.49.5.1857-1864.2005;
RA Kaatz G.W., McAleese F., Seo S.M.;
RT "Multidrug resistance in Staphylococcus aureus due to overexpression of a
RT novel multidrug and toxin extrusion (MATE) transport protein.";
RL Antimicrob. Agents Chemother. 49:1857-1864(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [3]
RP ROLE IN VIRULENCE.
RX PubMed=12368457; DOI=10.1099/00221287-148-10-3235;
RA Garvis S., Mei J.-M., Ruiz-Albert J., Holden D.W.;
RT "Staphylococcus aureus svrA: a gene required for virulence and expression
RT of the agr locus.";
RL Microbiology 148:3235-3243(2002).
RN [4]
RP INDUCTION.
RX PubMed=16569840; DOI=10.1128/aac.50.4.1276-1281.2006;
RA Kaatz G.W., DeMarco C.E., Seo S.M.;
RT "MepR, a repressor of the Staphylococcus aureus MATE family multidrug
RT efflux pump MepA, is a substrate-responsive regulatory protein.";
RL Antimicrob. Agents Chemother. 50:1276-1281(2006).
CC -!- FUNCTION: Multidrug resistance efflux protein involved in transporting
CC several clinically relevant monovalent and divalent biocides and the
CC fluoroquinolone antimicrobial agents norfloxacin and ciprofloxacin.
CC Required for transcription of agr and RNAIII and involved in the
CC production of protein A (spa) and toxins alpha, beta and delta.
CC {ECO:0000269|PubMed:12368457, ECO:0000269|PubMed:15855507}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Repressed by MepR. {ECO:0000269|PubMed:15855507,
CC ECO:0000269|PubMed:16569840}.
CC -!- SIMILARITY: Belongs to the multi antimicrobial extrusion (MATE) (TC
CC 2.A.66.1) family. MepA subfamily. {ECO:0000305}.
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DR EMBL; AY661734; AAU95768.1; -; Genomic_DNA.
DR EMBL; CP000253; ABD29484.1; -; Genomic_DNA.
DR RefSeq; WP_000651060.1; NZ_LS483365.1.
DR RefSeq; YP_498905.1; NC_007795.1.
DR AlphaFoldDB; Q2G140; -.
DR SMR; Q2G140; -.
DR STRING; 1280.SAXN108_0383; -.
DR ChEMBL; CHEMBL4295596; -.
DR TCDB; 2.A.66.1.13; the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.
DR EnsemblBacteria; ABD29484; ABD29484; SAOUHSC_00315.
DR GeneID; 3919542; -.
DR KEGG; sao:SAOUHSC_00315; -.
DR PATRIC; fig|93061.5.peg.287; -.
DR eggNOG; COG0534; Bacteria.
DR HOGENOM; CLU_012893_0_1_9; -.
DR OMA; MDGIWLA; -.
DR PRO; PR:Q2G140; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:InterPro.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR CDD; cd13143; MATE_MepA_like; 1.
DR InterPro; IPR002528; MATE_fam.
DR InterPro; IPR045070; MATE_MepA-like.
DR Pfam; PF01554; MatE; 2.
DR PIRSF; PIRSF006603; DinF; 1.
DR TIGRFAMs; TIGR00797; matE; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..451
FT /note="Multidrug export protein MepA"
FT /id="PRO_0000290234"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 397..417
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 451 AA; 48776 MW; 23BED4DB3B6BADB8 CRC64;
MKDEQLYYFE KSPVFKAMMH FSLPMMIGTL LSVIYGILNI YFIGFLEDSH MISAISLTLP
VFAILMGLGN LFGVGAGTYI SRLLGAKDYS KSKFVSSFSI YGGIALGLIV ILVTLPFSDQ
IAAILGARGE TLALTSNYLK VMFLSAPFVI LFFILEQFAR AIGAPMVSMI GMLASVGLNI
ILDPILIFGF DLNVVGAALG TAISNVAAAL FFIIYFMKNS DVVSVNIKLA KPNKEMLSEI
FKIGIPAFLM SILMGFTGLV LNLFLAHYGN FAIASYGISF RLVQFPELII MGLCEGVVPL
IAYNFMANKG RMKDVIKAVI MSIGVIFVVC MSAVFTIGHH MVGLFTTDQA IVEMATFILK
VTMASLLLNG IGFLFTGMLQ ATGQGRGATI MAILQGAIII PVLFIMNALF GLTGVIWSLL
IAESLCALAA MLIVYLLRDR LTVDTSELIE G