ID   MEPA_STAAN              Reviewed;         451 AA.
AC   Q7A7N0;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Multidrug export protein MepA;
GN   Name=mepA; OrderedLocusNames=SA0323;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N315;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   FUNCTION IN TIGECYCLINE RESISTANCE, AND INDUCTION.
RX   PubMed=15855508; DOI=10.1128/aac.49.5.1865-1871.2005;
RA   McAleese F., Petersen P., Ruzin A., Dunman P.M., Murphy E., Projan S.J.,
RA   Bradford P.A.;
RT   "A novel MATE family efflux pump contributes to the reduced susceptibility
RT   of laboratory-derived Staphylococcus aureus mutants to tigecycline.";
RL   Antimicrob. Agents Chemother. 49:1865-1871(2005).
CC   -!- FUNCTION: Multidrug resistance efflux protein (By similarity).
CC       Contributes to resistance to the glycylcycline antibiotic tigecycline.
CC       {ECO:0000250, ECO:0000269|PubMed:15855508}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Repressed by MepR. {ECO:0000269|PubMed:15855508}.
CC   -!- SIMILARITY: Belongs to the multi antimicrobial extrusion (MATE) (TC
CC       2.A.66.1) family. MepA subfamily. {ECO:0000305}.
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DR   EMBL; BA000018; BAB41547.1; -; Genomic_DNA.
DR   PIR; H89798; H89798.
DR   RefSeq; WP_000651051.1; NC_002745.2.
DR   AlphaFoldDB; Q7A7N0; -.
DR   SMR; Q7A7N0; -.
DR   EnsemblBacteria; BAB41547; BAB41547; BAB41547.
DR   KEGG; sau:SA0323; -.
DR   HOGENOM; CLU_012893_0_1_9; -.
DR   OMA; MDGIWLA; -.
DR   Proteomes; UP000000751; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:InterPro.
DR   GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   CDD; cd13143; MATE_MepA_like; 1.
DR   InterPro; IPR002528; MATE_fam.
DR   InterPro; IPR045070; MATE_MepA-like.
DR   Pfam; PF01554; MatE; 2.
DR   PIRSF; PIRSF006603; DinF; 1.
DR   TIGRFAMs; TIGR00797; matE; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..451
FT                   /note="Multidrug export protein MepA"
FT                   /id="PRO_0000290233"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        318..338
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        355..375
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        397..417
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        418..438
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   451 AA;  48816 MW;  99344DD83CA8E020 CRC64;
     MKDEQLYYFE KSPVFKAMMH FSLPMMIGTL LSVIYGILNI YFIGFLEDSH MISAISLTLP
     VFAILMGLGN LFGVGAGTYI SRLLGAKDYS KSKFVSSFSI YGGIALGLIV ILVTLPFSDQ
     IAAILGARGE TLALTSNYLK VMFLSAPFVI LFFILEQFAR AIGAPMISMI GMLASVGLNI
     ILDPILIFGF DLNVVGAALG TAISNVAAAL FFIIYFMKNS DVVSVNIKLA KPNKEMLSEI
     FKIGIPAFLM SILMGFTGLV LNLFLAHYGN FAIASYGISF RLVQFPELII MGLCEGVVPL
     IAYNFMANKG RMKDVIKAVI MSIGVIFVVC MIAVFTIGHH MVGLFTTDQA IVEMATFILK
     VTMASLLLNG IGFLFTGMLQ ATGQGRGATI MAILQGAIII PVLFIMNALF GLTGVIWSLL
     IAESLCALAA MLIVYLLRDR LTVDTSELIE G