ID   MEPB_PSEPU              Reviewed;        1050 AA.
AC   P0C070;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Multidrug/solvent efflux pump membrane transporter MepB;
GN   Name=mepB;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=KT2442-TOL;
RX   PubMed=9827328; DOI=10.1007/s007920050084;
RA   Fukumori F., Hirayama H., Takami H., Inoue A., Horikoshi K.;
RT   "Isolation and transposon mutagenesis of a Pseudomonas putida KT2442
RT   toluene-resistant variant: involvement of an efflux system in solvent
RT   resistance.";
RL   Extremophiles 2:395-400(1998).
CC   -!- FUNCTION: The inner membrane transporter component of an organic
CC       solvent and antibiotic efflux pump; confers resistance to toluene,
CC       hexane, p-xylene, ampicillin, penicillin G, erythromycin, novobiocin
CC       and tetracycline.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC       (TC 2.A.6) family. {ECO:0000305}.
CC   -!- CAUTION: There are 4 nearly identical operons in various strains of
CC       P.putida. This one and the ttgABC operon of strain DOT-T1E function in
CC       solvent and antibiotic efflux; however in strain S12 the arpABC operon
CC       functions only in antibiotic efflux. This may be due to different
CC       protein expression levels. In strain KT2440 the equivalent operon does
CC       not seem to function in toluene efflux. {ECO:0000305}.
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DR   RefSeq; WP_010952493.1; NZ_LT799039.1.
DR   AlphaFoldDB; P0C070; -.
DR   SMR; P0C070; -.
DR   PRIDE; P0C070; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0042908; P:xenobiotic transport; IEA:InterPro.
DR   Gene3D; 3.30.2090.10; -; 2.
DR   InterPro; IPR027463; AcrB_DN_DC_subdom.
DR   InterPro; IPR001036; Acrflvin-R.
DR   InterPro; IPR004764; HAE1.
DR   PANTHER; PTHR32063; PTHR32063; 1.
DR   Pfam; PF00873; ACR_tran; 1.
DR   PRINTS; PR00702; ACRIFLAVINRP.
DR   SUPFAM; SSF82714; SSF82714; 2.
DR   TIGRFAMs; TIGR00915; 2A0602; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1050
FT                   /note="Multidrug/solvent efflux pump membrane transporter
FT                   MepB"
FT                   /id="PRO_0000161851"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        339..359
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        370..390
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        393..413
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        440..460
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        472..492
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        539..559
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        871..891
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        893..913
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        923..943
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        972..992
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1004..1024
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1050 AA;  112836 MW;  B2F0872BE50A0E53 CRC64;
     MSKFFIDRPI FAWVIALVIM LVGALSILKL PINQYPSIAP PAIAIAVTYP GASAQTVQDT
     VVQVIEQQLN GIDNLRYVSS ESNSDGSMTI TATFEQGTNP DTAQVQVQNK LNLATPLLPQ
     EVQQQGIRVT KAVKNFLLVI GLVSEDGSMT KDDLANYIVS NMQDPISRTA GVGDFQVFGA
     QYAMRIWLDP AKLNKFQLTP VDVKTAVAAQ NVQVSSGQLG GLPALPGTQL NATIIGKTRL
     QTAEQFESIL LKVNKDGSQV RLGDVAQVGL GGENYAVSAQ FNGKPASGLA VKLATGANAL
     DTAKALRETI KGLEPFFPPG VKAVFPYDTT PVVTESISGV IHTLIEAVVL VFLVMYLFLQ
     NFRATIITTM TVPVVLLGTF GILAAAGFSI NTLTMFAMVL AIGLLVDDAI VVVENVERVM
     SEEGLPPKEA TKRSMEQIQG ALVGIALVLS AVLLPMAFFG GSTGVIYRQF SITIVSAMGL
     SVLVALIFTP ALCATMLKPL KKGEHHTAKG GFFGWFNRNF DRSVNGYERS VGAILRNKVP
     FLLAYALIVV GMIWLFARIP TAFLPEEDQG VLFAQVQTPA GSSAERTQVV VDQMREYLLK
     DEADTVSSVF TVNGFNFAGR GQSSGMAFIM LKPWDERSKE NSVFALAQRA QQHFFTFRDA
     MVFAFAPPAV LELGNATGFD VFLQDRGGVG HEKLMEARNQ FLAKAAQSKI LSAVRPNGLN
     DEPQYQLTID DERASALGVT IADINNTLSI ALGASYVNDF IDRGRVKKVY IQGEPSARMS
     PEDLQKWYVR NGAGEMVPFS SFAKGEWTYG SPKLSRYNGV EAMEILGAPA PGYSTGEAMA
     EVERIAGELP SGIGFSWTGM SYEEKLSGSQ MPALFALSVL FVFLCLAALY ESWSIPIAVV
     LVVPLGIIGA LIATSLRGLS NDVYFLVGLL TTIGLAAKNA ILIVEFAKEL HEQGRSLYDA
     AIEACRMRLR PIIMTSLAFI LGVVPLTIAS GAGAGSQHAI GTGVIGGMIS ATVLAIFWVP
     LFFVAVSSLF GSKEPEKDVT PENPRYEAGQ