ID   MEP_MELAF               Reviewed;          18 AA.
AC   P86170;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Melectin {ECO:0000303|PubMed:18942691};
DE            Short=MEP {ECO:0000303|PubMed:18942691};
OS   Melecta albifrons (Cuckoo bee) (Melecta punctata).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Melecta.
OX   NCBI_TaxID=582867;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS
RP   SPECTROMETRY, AND AMIDATION AT LYS-18.
RC   TISSUE=Venom {ECO:0000269|PubMed:18942691};
RX   PubMed=18942691; DOI=10.1002/cbic.200800476;
RA   Cerovsky V., Hovorka O., Cvacka J., Voburka Z., Bednarova L.,
RA   Borovickova L., Slaninova J., Fucik V.;
RT   "Melectin: a novel antimicrobial peptide from the venom of the
RT   cleptoparasitic bee Melecta albifrons.";
RL   ChemBioChem 9:2815-2821(2008).
CC   -!- FUNCTION: Antimicrobial activity against the Gram-positive bacteria
CC       B.subtilis (MIC=0.8 uM) and S.aureus (MIC=6.8 uM), and against the
CC       Gram-negative bacteria E.coli (MIC=2.0 uM) and P.aeruginosa (MIC=18.5
CC       uM). Stimulates degranulation from rat peritoneal mast cells with an
CC       EC(50) of 19.4 uM. Has very weak hemolytic activity towards rat
CC       erythrocytes. {ECO:0000269|PubMed:18942691}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18942691}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:18942691}.
CC   -!- MASS SPECTROMETRY: Mass=2038.24; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:18942691};
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DR   AlphaFoldDB; P86170; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:1990142; P:envenomation resulting in hemolysis in another organism; IDA:UniProtKB.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0035895; P:modulation of mast cell degranulation in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing;
KW   Hemolysis; Secreted.
FT   PEPTIDE         1..18
FT                   /note="Melectin"
FT                   /evidence="ECO:0000269|PubMed:18942691"
FT                   /id="PRO_0000363945"
FT   MOD_RES         18
FT                   /note="Lysine amide"
FT                   /evidence="ECO:0000269|PubMed:18942691"
SQ   SEQUENCE   18 AA;  2041 MW;  053CBB422FA4A8A6 CRC64;
     GFLSILKKVL PKVMAHMK