MER3_ARATH
ID MER3_ARATH Reviewed; 1133 AA.
AC Q5D892; A0A1I9LN52; Q9LVW8;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=DExH-box ATP-dependent RNA helicase DExH17 {ECO:0000305};
DE EC=3.6.4.13;
DE AltName: Full=ATP-dependent DNA helicase homolog MER3 {ECO:0000305};
DE EC=3.6.4.12;
DE AltName: Full=Protein ROCK-N-ROLLERS {ECO:0000303|PubMed:16045469};
GN Name=MER3 {ECO:0000303|PubMed:15854901};
GN Synonyms=RCK {ECO:0000303|PubMed:16045469};
GN OrderedLocusNames=At3g27730 {ECO:0000312|Araport:AT3G27730};
GN ORFNames=MGF10.14 {ECO:0000312|EMBL:BAB02697.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=15854901; DOI=10.1016/j.cub.2005.02.056;
RA Mercier R., Jolivet S., Vezon D., Huppe E., Chelysheva L., Giovanni M.,
RA Nogue F., Doutriaux M.-P., Horlow C., Grelon M., Mezard C.;
RT "Two meiotic crossover classes cohabit in Arabidopsis: one is dependent on
RT MER3, whereas the other one is not.";
RL Curr. Biol. 15:692-701(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=16045469; DOI=10.1111/j.1365-313x.2005.02461.x;
RA Chen C., Zhang W., Timofejeva L., Gerardin Y., Ma H.;
RT "The Arabidopsis ROCK-N-ROLLERS gene encodes a homolog of the yeast ATP-
RT dependent DNA helicase MER3 and is required for normal meiotic crossover
RT formation.";
RL Plant J. 43:321-334(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP GENE FAMILY.
RX PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA Xu R., Zhang S., Huang J., Zheng C.;
RT "Genome-wide comparative in silico analysis of the RNA helicase gene family
RT in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza
RT sativa.";
RL PLoS ONE 8:E78982-E78982(2013).
CC -!- FUNCTION: DNA helicase required for crossover formation, complete
CC synapsis of homologous chromosomes and bivalent formation during
CC meiosis. Is specific to recombination events resulting in interference-
CC sensitive crossovers (class I meiotic crossover).
CC {ECO:0000269|PubMed:15854901, ECO:0000269|PubMed:16045469}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000305};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in meiocytes during meiosis.
CC {ECO:0000269|PubMed:15854901, ECO:0000269|PubMed:16045469}.
CC -!- DISRUPTION PHENOTYPE: Low levels of fertility due to a significant
CC decrease of meiotic crossovers. {ECO:0000269|PubMed:15854901,
CC ECO:0000269|PubMed:16045469}.
CC -!- SIMILARITY: Belongs to the DExH box helicase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02697.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY822649; AAX14498.1; -; mRNA.
DR EMBL; AY960701; AAX58606.1; -; mRNA.
DR EMBL; AB018114; BAB02697.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE77357.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64007.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64010.1; -; Genomic_DNA.
DR RefSeq; NP_001326060.1; NM_001338909.1.
DR RefSeq; NP_001326063.1; NM_001338910.1.
DR RefSeq; NP_189410.2; NM_113689.2.
DR AlphaFoldDB; Q5D892; -.
DR SMR; Q5D892; -.
DR STRING; 3702.AT3G27730.1; -.
DR PaxDb; Q5D892; -.
DR PRIDE; Q5D892; -.
DR EnsemblPlants; AT3G27730.1; AT3G27730.1; AT3G27730.
DR EnsemblPlants; AT3G27730.6; AT3G27730.6; AT3G27730.
DR EnsemblPlants; AT3G27730.7; AT3G27730.7; AT3G27730.
DR GeneID; 822395; -.
DR Gramene; AT3G27730.1; AT3G27730.1; AT3G27730.
DR Gramene; AT3G27730.6; AT3G27730.6; AT3G27730.
DR Gramene; AT3G27730.7; AT3G27730.7; AT3G27730.
DR KEGG; ath:AT3G27730; -.
DR Araport; AT3G27730; -.
DR TAIR; locus:2089144; AT3G27730.
DR eggNOG; KOG0952; Eukaryota.
DR HOGENOM; CLU_000335_0_2_1; -.
DR InParanoid; Q5D892; -.
DR PhylomeDB; Q5D892; -.
DR PRO; PR:Q5D892; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q5D892; baseline and differential.
DR Genevisible; Q5D892; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; ISS:TAIR.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051026; P:chiasma assembly; IMP:TAIR.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:TAIR.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004179; Sec63-dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF02889; Sec63; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00973; Sec63; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; DNA-binding; Helicase; Hydrolase; Meiosis; Nucleotide-binding;
KW Nucleus; Reference proteome; RNA-binding; rRNA-binding.
FT CHAIN 1..1133
FT /note="DExH-box ATP-dependent RNA helicase DExH17"
FT /id="PRO_0000432116"
FT DOMAIN 34..229
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 263..460
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT DOMAIN 536..847
FT /note="SEC63"
FT /evidence="ECO:0000255"
FT REGION 878..919
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 165..168
FT /note="DEVH box"
FT /evidence="ECO:0000305"
FT COMPBIAS 889..912
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 47..54
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1133 AA; 128290 MW; 07583DBD0D5BAB4B CRC64;
MDTHTLKSVS DLPGNFRSAF SFRYFNSLQS ECFPLCFHSD INMIISAPTG SGKTVLFELC
ILRLFSKSIS KEGSFLHAKG ALKTVYISPS KALVQEKLRD WNQKFNSWGI SCLELTGDNE
TYSTKNIQDA DIILTTPEKF DAVSRYRVTS GGLGFFSDIA LVLIDEVHLL NDPRGAALEA
IVSRLKILSS NHELRSSTLA SVRLLAVSAT IPNIEDLAEW LKVPTAGIKR FGEEMRPVKL
TTKVFGYAAA KNDFLFEKRL QNYIYDILMQ YSKGKSALVF CSTRKGAQEA AQKLAQTAMT
YGYSNPFIKS REQLERLREA SPMCSDKQMQ SYILQGVGYH NGGLCQKDRS LVEGLFLNGD
IQVICTTNTL AHGINLPAHT VVIKSTQHFN KEKGHYMEYD RSTLLQMSGR AGRPPFDDTG
MVIIMTRRET VHLYENLLNG CEVVESQLLP CLIEHLTAEI VQLTISDITR AIEWMKCSYL
YVRMKKNPEN YAIKKGIPKD RVEKHLQELC LQKINELSQY QMIWTDTDGF VLKPEEPGRL
MTKYYLKFET MKYIINTPTS YSLDEALHIV CHAEEISWIQ LRRNEKKTLN DVNADKEGRL
RFHINDNKGK RKKRIQTREE KLFVLANDWL TGDPSVHDLS MTQDANSICS NGSRIARCMK
EYFIYKKNYK GTLSSTLLAK SLYQKLWDDS PYLLKQLPGI GMVTAKALHS MGVRSFEALA
EADPRRIEIV TGRKYPFGNT IKESLSSLPP KVEIKVEEVD CQKQGISKLA VTLSRVSQPL
QSTKRHYADL IVGSEEENLI HFHEKIRMED FSSPYSVTVL LERPHQQTKV TVKADLIFEE
YIGIDLHETL LLKKANNNKV NYKSENRMPQ YYPPMASACI ADDDNPVTSG PSNRKDKKDD
MPSFKLIDDD SEEEKEPYVT MEEDDCVIIN EHTVFDHIRE KAKCFPSLNP LNPTSSPASG
KSILKRKSLV ENNSPELDPL FQYDSVFDLP TNTKDIKQSA QQITSPGYAS FAEKTETERP
FSDETIFNYI RKRSKNSPAL ATSKIENPIT ISSQEGRNAE ISPYRTYGLL VSPATKIPRI
TSDAPSEILS FDISMVKRSD TSLEQTKGFC STLAGKSNVS DSFLGFKSIF SFL
