MER3_ORYSJ
ID MER3_ORYSJ Reviewed; 1205 AA.
AC B6DMK2; Q0DZI2;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=ATP-dependent DNA helicase MER3 homolog {ECO:0000305};
DE EC=3.6.4.12 {ECO:0000305};
DE AltName: Full=Protein RICE MEIOTIC CROSSOVER 1 {ECO:0000303|PubMed:19470578};
GN Name=MER3 {ECO:0000303|PubMed:19470578};
GN Synonyms=RMC1 {ECO:0000312|EMBL:ACI28521.1};
GN OrderedLocusNames=Os02g0617500 {ECO:0000312|EMBL:BAF09356.1},
GN LOC_Os02g40450 {ECO:0000305};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Nipponbare;
RX PubMed=19470578; DOI=10.1242/jcs.049080;
RA Wang K., Tang D., Wang M., Lu J., Yu H., Liu J., Qian B., Gong Z., Wang X.,
RA Chen J., Gu M., Cheng Z.;
RT "MER3 is required for normal meiotic crossover formation, but not for
RT presynaptic alignment in rice.";
RL J. Cell Sci. 122:2055-2063(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP FUNCTION.
RX PubMed=19417775; DOI=10.1038/cr.2009.52;
RA Chang L., Ma H., Xue H.W.;
RT "Functional conservation of the meiotic genes SDS and RCK in male meiosis
RT in the monocot rice.";
RL Cell Res. 19:768-782(2009).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=22393242; DOI=10.1242/jcs.090993;
RA Shen Y., Tang D., Wang K., Wang M., Huang J., Luo W., Luo Q., Hong L.,
RA Li M., Cheng Z.;
RT "ZIP4 in homologous chromosome synapsis and crossover formation in rice
RT meiosis.";
RL J. Cell Sci. 125:2581-2591(2012).
CC -!- FUNCTION: DNA helicase required for crossover formation, complete
CC synapsis of homologous chromosomes and bivalent formation during
CC meiosis. Is specific to recombination events resulting in interference-
CC sensitive crossovers (class I meiotic crossover) (PubMed:19470578,
CC PubMed:19417775). Works cooperatively with ZIP4 to promote crossovers
CC (PubMed:22393242). {ECO:0000269|PubMed:19417775,
CC ECO:0000269|PubMed:19470578, ECO:0000269|PubMed:22393242}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22393242}. Chromosome
CC {ECO:0000269|PubMed:22393242}. Note=Detected in punctuate foci onto the
CC chromosomes in prophase I meiocytes. {ECO:0000269|PubMed:22393242}.
CC -!- DISRUPTION PHENOTYPE: Complete loss of fertility due to defect in
CC meiosis. {ECO:0000269|PubMed:19470578}.
CC -!- SIMILARITY: Belongs to the helicase family. SKI2 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF09356.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; FJ008126; ACI28521.1; -; mRNA.
DR EMBL; AP003980; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP008208; BAF09356.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; B6DMK2; -.
DR SMR; B6DMK2; -.
DR STRING; 4530.OS02T0617500-01; -.
DR PaxDb; B6DMK2; -.
DR PRIDE; B6DMK2; -.
DR eggNOG; KOG0952; Eukaryota.
DR HOGENOM; CLU_000335_0_2_1; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; B6DMK2; OS.
DR GO; GO:0005694; C:chromosome; IMP:UniProtKB.
DR GO; GO:0005634; C:nucleus; IMP:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004179; Sec63-dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF02889; Sec63; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00973; Sec63; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chromosome; DNA-binding; Helicase; Hydrolase; Meiosis;
KW Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..1205
FT /note="ATP-dependent DNA helicase MER3 homolog"
FT /id="PRO_0000432117"
FT DOMAIN 41..236
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 266..467
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT DOMAIN 541..852
FT /note="SEC63"
FT /evidence="ECO:0000255"
FT REGION 1075..1131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 172..175
FT /note="DEAH box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT COMPBIAS 1075..1095
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1097..1126
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 54..61
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 1205 AA; 135183 MW; 6C8858DB72BA1E9F CRC64;
MAAMGHLGDP YALRSVADLP PPFRSVFGFR YFNSLQSECF PACFLSDVNM VISAPTGSGK
TVLFELCILR LLSRFLSSEW RFNLIKGTLK TIYIAPMKAL VQEKLRDWNM KLGSLGISCL
EMTGDNEFYN TKSIHDADLI LTTPEKFDSV SRHGIRDGGL GFFSDIALVL IDEVHLLNDP
RGAALEAIVS RIKMLSRLGT MKIAPLANVR FIAVSATIPN IEDIAEWLAV PSEGIKRFGE
EMRPVKLTTK VFGYAPARND FLFERRLQSF IFDILMQHSR GKSALVFCST RKGAQEAAQC
LSQTASSLGY SNPFMKSMQQ YEHLKEAALT CSDKQLQACL VHGVGYHNGG LCLKDRSVVE
GLFLKGDIQI LCTTNTLAHG INLPAHTVVI KSTQFFNKEK GLYVEYERSM VLQMCGRAGR
PPFDDTGTII IMTRRETVHL YENLLNGCEM VESQLLPCAV EHLNAEIVQL TVSDITLAIE
WLKCSYLYIR IKKNPQHYGI KKEIPRELLE KQMKDICVEK IHELGEYGLI WTDEDGFLLK
PLEPGRLMTK FYLKFDTMKL IVKASACCTL EDLLHIICHS AEITWIQLRR NEKKLLNEIN
ADKEGRLWFH VVGANGKRKK RIQTREEKIF ILANDCLTGD PLVHDLSLNQ EMNSICSNGC
RVAKCMREYF IYKKNYKSAI SSMLLAKCLH QKLWESSPFL LKQLPGIGIV TAKALKTAGI
DSFESLATAD ARKIESVTGR NYPFGDSIKS YLPSLGPKID INIEDAGNRQ GKSTIIVTLT
RLSQAVGSSK QNYADMVVGS EEDNAILFHE KIKTQEFSSP YSVKLYVPCP PNARATLKVD
VIFEEYVGLD IHKKHVVSRE DFHVTKVFGI KKAEPLYNLP AESCLVSSKT TRTNQSKYHN
GQNPLSKEVC VIEDDFRAKA PDKDDNDLEI LGTREYNNLA SLEAPSFTLL HEEDYEDVPD
VLASEPVEAE CKSATNNTIF DHIRKKSRDF PNLMLSKSMD SSYEPLILKK MKTSGDQFGL
DQSSLHAYEV TPMVFDRTEA RVSPNNTDER CRNILTRTAE TRSFQFTGKM DSISQKSEIL
NRTQGKNSTQ LAGKKAFEKS KTPDENSLHF VGKRDSSSEK SKALSKTPDE NSLQFLGKMD
SSSEKSKFCF SSPLADFQPM QCTKQVAASV QPLTIQDYCK DILASAKSSG TGASFLDVKS
VFSFL
