ID   MERB_BACCE              Reviewed;         218 AA.
AC   P16172; Q93R38;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   26-SEP-2003, sequence version 2.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Alkylmercury lyase;
DE            EC=4.99.1.2;
DE   AltName: Full=Organomercurial lyase;
GN   Name=merB1; Synonyms=merB;
OS   Bacillus cereus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1396;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RC607; TRANSPOSON=Tn5084;
RX   PubMed=2536669; DOI=10.1128/jb.171.1.83-92.1989;
RA   Wang Y., Moore M., Levinson H.S., Silver S., Walsh C.T., Mahler I.;
RT   "Nucleotide sequence of a chromosomal mercury resistance determinant from a
RT   Bacillus sp. with broad-spectrum mercury resistance.";
RL   J. Bacteriol. 171:83-92(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RC607; TRANSPOSON=Tn5084;
RX   PubMed=10559175; DOI=10.1128/jb.181.22.7080-7086.1999;
RA   Gupta A., Phung L.T., Chakravarty L., Silver S.;
RT   "Mercury resistance in Bacillus cereus RC607: transcriptional organization
RT   and two new open reading frames.";
RL   J. Bacteriol. 181:7080-7086(1999).
CC   -!- FUNCTION: Cleaves the carbon-mercury bond of organomercurials such as
CC       phenylmercuric acetate. One product is Hg(2+), which is subsequently
CC       detoxified by the mercuric reductase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkylmercury + H(+) = an alkane + Hg(2+);
CC         Xref=Rhea:RHEA:18777, ChEBI:CHEBI:15378, ChEBI:CHEBI:16793,
CC         ChEBI:CHEBI:18310, ChEBI:CHEBI:83725; EC=4.99.1.2;
CC   -!- SIMILARITY: Belongs to the MerB family. {ECO:0000305}.
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DR   EMBL; AF138877; AAA83978.1; -; Genomic_DNA.
DR   EMBL; AB066362; BAB62436.1; -; Genomic_DNA.
DR   RefSeq; WP_000845541.1; NZ_VEGO01000042.1.
DR   AlphaFoldDB; P16172; -.
DR   SMR; P16172; -.
DR   BRENDA; 4.99.1.2; 648.
DR   GO; GO:0018836; F:alkylmercury lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046413; P:organomercury catabolic process; IEA:InterPro.
DR   GO; GO:0046689; P:response to mercury ion; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00714; MerB; 1.
DR   InterPro; IPR004927; MerB.
DR   InterPro; IPR024259; MerB_HTH_dom.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF12324; HTH_15; 1.
DR   Pfam; PF03243; MerB; 1.
DR   PIRSF; PIRSF001458; MerB; 1.
DR   PRINTS; PR01699; ORGNOHGLYASE.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   3: Inferred from homology;
KW   Lyase; Mercuric resistance; Mercury; Transposable element.
FT   CHAIN           1..218
FT                   /note="Alkylmercury lyase"
FT                   /id="PRO_0000220354"
FT   CONFLICT        217
FT                   /note="D -> H (in Ref. 1; AAA83978)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   218 AA;  23863 MW;  302ABC8DF734B8C4 CRC64;
     MKTEIQEIVT RLDQQSNKGE GGESMKWLFR PLLQMLAGGE SVTIEDMATT TGKPVEEVKK
     VLQSLPSVEI DEQGRVVGLG LTLIPTPHHF TVDGKQLYAW CALDTLIFPA LIGRSVNIES
     PCHSTGEPIR LNVEPDHIVS VEPSTAVVSI VTPDDMSSIR TAFCNEVHFF SSPNAAEDWL
     DQHPGGKVLS VEDAFELGRL MGTRYEESRP ANGSCCDI