ID   MERB_CLOBU              Reviewed;         218 AA.
AC   P62224;
DT   05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Alkylmercury lyase {ECO:0000255|HAMAP-Rule:MF_00714};
DE            EC=4.99.1.2 {ECO:0000255|HAMAP-Rule:MF_00714};
DE   AltName: Full=Organomercurial lyase {ECO:0000255|HAMAP-Rule:MF_00714};
GN   Name=merB {ECO:0000255|HAMAP-Rule:MF_00714};
OS   Clostridium butyricum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1492;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Mersaru;
RA   Narita M., Koizumi T., Huang C., Endo G.;
RT   "Broad-spectrum mercury resistance and its genetic characterization of
RT   anaerobic mercury-resistant bacterium, Clostridium butyricum Mersaru,
RT   isolated from sediment of Minamata bay, Japan.";
RL   Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cleaves the carbon-mercury bond of organomercurials such as
CC       phenylmercuric acetate. One product is Hg(2+), which is subsequently
CC       detoxified by the mercuric reductase. {ECO:0000255|HAMAP-
CC       Rule:MF_00714}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkylmercury + H(+) = an alkane + Hg(2+);
CC         Xref=Rhea:RHEA:18777, ChEBI:CHEBI:15378, ChEBI:CHEBI:16793,
CC         ChEBI:CHEBI:18310, ChEBI:CHEBI:83725; EC=4.99.1.2;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00714};
CC   -!- SIMILARITY: Belongs to the MerB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00714}.
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DR   EMBL; AB024961; BAA86120.1; -; Genomic_DNA.
DR   AlphaFoldDB; P62224; -.
DR   SMR; P62224; -.
DR   GO; GO:0018836; F:alkylmercury lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046413; P:organomercury catabolic process; IEA:InterPro.
DR   GO; GO:0046689; P:response to mercury ion; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00714; MerB; 1.
DR   InterPro; IPR004927; MerB.
DR   InterPro; IPR024259; MerB_HTH_dom.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF12324; HTH_15; 1.
DR   Pfam; PF03243; MerB; 1.
DR   PIRSF; PIRSF001458; MerB; 1.
DR   PRINTS; PR01699; ORGNOHGLYASE.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   3: Inferred from homology;
KW   Lyase; Mercuric resistance; Mercury.
FT   CHAIN           1..218
FT                   /note="Alkylmercury lyase"
FT                   /id="PRO_0000220355"
SQ   SEQUENCE   218 AA;  23885 MW;  3021FC8DF734B8C4 CRC64;
     MKTEIQEIVT RLDQQSNKGE GGESMKWLFR PLLQMLAGGE SVTIEDMATT TGKPVEEVKK
     VLQSLPSVEI DEQGRVVGLG LTLIPTPHHF TVDGKQLYAW CALDTLIFPA LIGRSVNIES
     PCHSTGEPIR LNVEPDHIVS VEPSTAVVSI VTPDDMSSIR TAFCNEVHFF SSPNAAEDWL
     DQHPGGKVLS VEDAFELGRL MGTRYEESRP ANGSCCHI