MERB_ECOLX
ID MERB_ECOLX Reviewed; 212 AA.
AC P77072;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Alkylmercury lyase;
DE EC=4.99.1.2;
DE AltName: Full=Organomercurial lyase;
GN Name=merB;
OS Escherichia coli.
OG Plasmid IncM R831b.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Tolle C., Totis P., Summers A.O.;
RT "Nucleotide sequence of the organomercury resistance (OMR) locus.";
RL Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cleaves the carbon-mercury bond of organomercurials such as
CC phenylmercuric acetate. One product is Hg(2+), which is subsequently
CC detoxified by the mercuric reductase (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an alkylmercury + H(+) = an alkane + Hg(2+);
CC Xref=Rhea:RHEA:18777, ChEBI:CHEBI:15378, ChEBI:CHEBI:16793,
CC ChEBI:CHEBI:18310, ChEBI:CHEBI:83725; EC=4.99.1.2;
CC -!- SIMILARITY: Belongs to the MerB family. {ECO:0000305}.
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DR EMBL; U77087; AAB49639.1; -; Genomic_DNA.
DR PDB; 1S6L; NMR; -; A=1-212.
DR PDB; 3F0O; X-ray; 1.76 A; A/B=1-212.
DR PDB; 3F0P; X-ray; 1.64 A; A/B=1-212.
DR PDB; 3F2F; X-ray; 1.98 A; A/B=1-212.
DR PDB; 3F2G; X-ray; 1.78 A; A/B=1-212.
DR PDB; 3F2H; X-ray; 2.00 A; A/B=1-212.
DR PDB; 3FN8; X-ray; 1.88 A; A/B=1-212.
DR PDB; 5C0T; X-ray; 1.96 A; A/B=1-212.
DR PDB; 5C0U; X-ray; 1.87 A; A/B=1-212.
DR PDB; 5DSF; X-ray; 1.95 A; A/B=1-212.
DR PDB; 5U79; X-ray; 1.60 A; A/B=1-212.
DR PDB; 5U7A; X-ray; 1.53 A; A/B=1-212.
DR PDB; 5U7B; X-ray; 2.00 A; A/B=1-212.
DR PDB; 5U7C; X-ray; 1.75 A; A/B=1-212.
DR PDB; 5U82; X-ray; 1.85 A; A/B=1-212.
DR PDB; 5U83; X-ray; 1.61 A; A/B=1-212.
DR PDB; 5U88; X-ray; 1.80 A; A/B=1-212.
DR PDBsum; 1S6L; -.
DR PDBsum; 3F0O; -.
DR PDBsum; 3F0P; -.
DR PDBsum; 3F2F; -.
DR PDBsum; 3F2G; -.
DR PDBsum; 3F2H; -.
DR PDBsum; 3FN8; -.
DR PDBsum; 5C0T; -.
DR PDBsum; 5C0U; -.
DR PDBsum; 5DSF; -.
DR PDBsum; 5U79; -.
DR PDBsum; 5U7A; -.
DR PDBsum; 5U7B; -.
DR PDBsum; 5U7C; -.
DR PDBsum; 5U82; -.
DR PDBsum; 5U83; -.
DR PDBsum; 5U88; -.
DR AlphaFoldDB; P77072; -.
DR BMRB; P77072; -.
DR SMR; P77072; -.
DR BioCyc; MetaCyc:MON-3304; -.
DR BRENDA; 4.99.1.2; 2026.
DR EvolutionaryTrace; P77072; -.
DR GO; GO:0018836; F:alkylmercury lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046413; P:organomercury catabolic process; IEA:InterPro.
DR GO; GO:0046689; P:response to mercury ion; IEA:UniProtKB-UniRule.
DR DisProt; DP00575; -.
DR HAMAP; MF_00714; MerB; 1.
DR InterPro; IPR004927; MerB.
DR InterPro; IPR024259; MerB_HTH_dom.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF12324; HTH_15; 1.
DR Pfam; PF03243; MerB; 1.
DR PIRSF; PIRSF001458; MerB; 1.
DR PRINTS; PR01699; ORGNOHGLYASE.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Lyase; Mercuric resistance; Mercury; Plasmid.
FT CHAIN 1..212
FT /note="Alkylmercury lyase"
FT /id="PRO_0000220356"
FT HELIX 4..14
FT /evidence="ECO:0007829|PDB:5U7A"
FT HELIX 20..31
FT /evidence="ECO:0007829|PDB:5U7A"
FT TURN 32..34
FT /evidence="ECO:0007829|PDB:5U7A"
FT HELIX 39..46
FT /evidence="ECO:0007829|PDB:5U7A"
FT HELIX 50..59
FT /evidence="ECO:0007829|PDB:5U7A"
FT TURN 67..69
FT /evidence="ECO:0007829|PDB:1S6L"
FT STRAND 71..79
FT /evidence="ECO:0007829|PDB:5U7A"
FT STRAND 82..87
FT /evidence="ECO:0007829|PDB:5U7A"
FT STRAND 90..96
FT /evidence="ECO:0007829|PDB:5U7A"
FT HELIX 97..107
FT /evidence="ECO:0007829|PDB:5U7A"
FT STRAND 111..116
FT /evidence="ECO:0007829|PDB:5U7A"
FT TURN 118..120
FT /evidence="ECO:0007829|PDB:5U7A"
FT STRAND 123..128
FT /evidence="ECO:0007829|PDB:5U7A"
FT STRAND 133..138
FT /evidence="ECO:0007829|PDB:5U7A"
FT STRAND 142..145
FT /evidence="ECO:0007829|PDB:5U7A"
FT STRAND 150..152
FT /evidence="ECO:0007829|PDB:3F0P"
FT HELIX 155..161
FT /evidence="ECO:0007829|PDB:5U7A"
FT STRAND 164..166
FT /evidence="ECO:0007829|PDB:5U7A"
FT HELIX 168..176
FT /evidence="ECO:0007829|PDB:5U7A"
FT STRAND 180..182
FT /evidence="ECO:0007829|PDB:1S6L"
FT STRAND 185..188
FT /evidence="ECO:0007829|PDB:5U7A"
FT HELIX 189..205
FT /evidence="ECO:0007829|PDB:5U7A"
SQ SEQUENCE 212 AA; 23035 MW; 7FE9DFA8A95F490A CRC64;
MKLAPYILEL LTSVNRTNGT ADLLVPLLRE LAKGRPVSRT TLAGILDWPA ERVAAVLEQA
TSTEYDKDGN IIGYGLTLRE TSYVFEIDDR RLYAWCALDT LIFPALIGRT ARVSSHCAAT
GAPVSLTVSP SEIQAVEPAG MAVSLVLPQE AADVRQSFCC HVHFFASVPT AEDWASKHQG
LEGLAIVSVH EAFGLGQEFN RHLLQTMSSR TP
