ID   MERB_PSEPU              Reviewed;         212 AA.
AC   Q8G9P0;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Alkylmercury lyase;
DE            EC=4.99.1.2;
DE   AltName: Full=Organomercurial lyase;
GN   Name=merB;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MU10-2; TRANSPOSON=Tn5041D;
RX   PubMed=12427948; DOI=10.1099/00221287-148-11-3569;
RA   Kholodii G., Gorlenko Z., Mindlin S., Hobman J., Nikiforov V.;
RT   "Tn5041-like transposons: molecular diversity, evolutionary relationships
RT   and distribution of distinct variants in environmental bacteria.";
RL   Microbiology 148:3569-3582(2002).
CC   -!- FUNCTION: Cleaves the carbon-mercury bond of organomercurials such as
CC       phenylmercuric acetate. One product is Hg(2+), which is subsequently
CC       detoxified by the mercuric reductase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkylmercury + H(+) = an alkane + Hg(2+);
CC         Xref=Rhea:RHEA:18777, ChEBI:CHEBI:15378, ChEBI:CHEBI:16793,
CC         ChEBI:CHEBI:18310, ChEBI:CHEBI:83725; EC=4.99.1.2;
CC   -!- SIMILARITY: Belongs to the MerB family. {ECO:0000305}.
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DR   EMBL; AJ318529; CAC86907.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8G9P0; -.
DR   SMR; Q8G9P0; -.
DR   GO; GO:0018836; F:alkylmercury lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046413; P:organomercury catabolic process; IEA:InterPro.
DR   GO; GO:0046689; P:response to mercury ion; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00714; MerB; 1.
DR   InterPro; IPR004927; MerB.
DR   InterPro; IPR024259; MerB_HTH_dom.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF12324; HTH_15; 1.
DR   Pfam; PF03243; MerB; 1.
DR   PIRSF; PIRSF001458; MerB; 1.
DR   PRINTS; PR01699; ORGNOHGLYASE.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   3: Inferred from homology;
KW   Lyase; Mercuric resistance; Mercury; Transposable element.
FT   CHAIN           1..212
FT                   /note="Alkylmercury lyase"
FT                   /id="PRO_0000220358"
SQ   SEQUENCE   212 AA;  22981 MW;  433D4DCB870B5ADA CRC64;
     MDKTIYSKKI AESLSSGNHP EEFATLFVTL LRQLVMGRPV SREALGTALG WSGQRVATVL
     EPAPGTEYDE QGNIIGLGLT LRETPHVFEV DGRRLYTWCA LDTLMFPALI GKTARVTSRC
     VATGRPITLT VAPEAVLQVE PAETMVSLLT PDASPDIRCS FCCHVHFFAS PSVANAWAST
     HPGIELVPVE NAFGLGHLIA HKLLEDSERN TA