ID   MERB_STAAU              Reviewed;         216 AA.
AC   P08653;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Alkylmercury lyase;
DE            EC=4.99.1.2;
DE   AltName: Full=Organomercurial lyase;
GN   Name=merB;
OS   Staphylococcus aureus.
OG   Plasmid pI258.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3037534; DOI=10.1073/pnas.84.15.5106;
RA   Laddaga R.A., Chu L., Misra T.K., Silver S.;
RT   "Nucleotide sequence and expression of the mercurial-resistance operon from
RT   Staphylococcus aureus plasmid pI258.";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:5106-5110(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=85/2082;
RX   PubMed=11302791; DOI=10.1128/aac.45.5.1323-1336.2001;
RA   Ito T., Katayama Y., Asada K., Mori N., Tsutsumimoto K.;
RT   "Structural comparison of three types of staphylococcal cassette chromosome
RT   mec integrated in the chromosome in methicillin-resistant Staphylococcus
RT   aureus.";
RL   Antimicrob. Agents Chemother. 45:1323-1336(2001).
CC   -!- FUNCTION: Cleaves the carbon-mercury bond of organomercurials such as
CC       phenylmercuric acetate. One product is Hg(2+), which is subsequently
CC       detoxified by the mercuric reductase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkylmercury + H(+) = an alkane + Hg(2+);
CC         Xref=Rhea:RHEA:18777, ChEBI:CHEBI:15378, ChEBI:CHEBI:16793,
CC         ChEBI:CHEBI:18310, ChEBI:CHEBI:83725; EC=4.99.1.2;
CC   -!- SIMILARITY: Belongs to the MerB family. {ECO:0000305}.
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DR   EMBL; L29436; AAA98246.1; -; Genomic_DNA.
DR   EMBL; AB037671; BAB47641.1; -; Genomic_DNA.
DR   PIR; F29504; F29504.
DR   RefSeq; WP_000790333.1; NZ_WWCF01000007.1.
DR   RefSeq; YP_006937592.1; NC_013319.1.
DR   RefSeq; YP_006937779.1; NC_013323.1.
DR   RefSeq; YP_006938294.1; NC_013337.1.
DR   RefSeq; YP_006938627.1; NC_013347.1.
DR   RefSeq; YP_006938789.1; NC_013352.1.
DR   AlphaFoldDB; P08653; -.
DR   SMR; P08653; -.
DR   GO; GO:0018836; F:alkylmercury lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046413; P:organomercury catabolic process; IEA:InterPro.
DR   GO; GO:0046689; P:response to mercury ion; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00714; MerB; 1.
DR   InterPro; IPR004927; MerB.
DR   InterPro; IPR024259; MerB_HTH_dom.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF12324; HTH_15; 1.
DR   Pfam; PF03243; MerB; 1.
DR   PIRSF; PIRSF001458; MerB; 1.
DR   PRINTS; PR01699; ORGNOHGLYASE.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   3: Inferred from homology;
KW   Lyase; Mercuric resistance; Mercury; Plasmid.
FT   CHAIN           1..216
FT                   /note="Alkylmercury lyase"
FT                   /id="PRO_0000220361"
SQ   SEQUENCE   216 AA;  23645 MW;  45093644B225D97F CRC64;
     MKNISEFSAQ LDQTFDQGEA VSMEWLFRPL LKMLAEGDPV PVEDIAAETG KPVEEVKQVL
     QTLPSVELDE QGRVVGYGLT LFPTPHRFEV DGKQLYAWCA LDTLMFPALI GRTVHIASPC
     HGTGKSVRLT VEPDRVVSVE PSTAVVSIVT PDEMASVRSA FCNDVHFFSS PSAAQDWLNQ
     HPESSVLPVE DAFELGRHLG ARYEESGPTN GSCCNI