ID   MERB_STAES              Reviewed;         216 AA.
AC   Q8CU52;
DT   26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Alkylmercury lyase;
DE            EC=4.99.1.2;
DE   AltName: Full=Organomercurial lyase;
GN   Name=merB; OrderedLocusNames=SE_0086;
OS   Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12228 / FDA PCI 1200;
RX   PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q.,
RA   Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H.,
RA   Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- FUNCTION: Cleaves the carbon-mercury bond of organomercurials such as
CC       phenylmercuric acetate. One product is Hg(2+), which is subsequently
CC       detoxified by the mercuric reductase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkylmercury + H(+) = an alkane + Hg(2+);
CC         Xref=Rhea:RHEA:18777, ChEBI:CHEBI:15378, ChEBI:CHEBI:16793,
CC         ChEBI:CHEBI:18310, ChEBI:CHEBI:83725; EC=4.99.1.2;
CC   -!- SIMILARITY: Belongs to the MerB family. {ECO:0000305}.
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DR   EMBL; AE015929; AAO03683.1; -; Genomic_DNA.
DR   RefSeq; NP_763641.1; NC_004461.1.
DR   RefSeq; WP_000790334.1; NZ_WBME01000076.1.
DR   AlphaFoldDB; Q8CU52; -.
DR   SMR; Q8CU52; -.
DR   STRING; 176280.SE_0086; -.
DR   EnsemblBacteria; AAO03683; AAO03683; SE_0086.
DR   GeneID; 50017514; -.
DR   KEGG; sep:SE_0086; -.
DR   PATRIC; fig|176280.10.peg.82; -.
DR   eggNOG; COG1249; Bacteria.
DR   HOGENOM; CLU_101685_0_0_9; -.
DR   OMA; TWCALDA; -.
DR   Proteomes; UP000001411; Chromosome.
DR   GO; GO:0018836; F:alkylmercury lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046413; P:organomercury catabolic process; IEA:InterPro.
DR   GO; GO:0046689; P:response to mercury ion; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00714; MerB; 1.
DR   InterPro; IPR004927; MerB.
DR   InterPro; IPR024259; MerB_HTH_dom.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF12324; HTH_15; 1.
DR   Pfam; PF03243; MerB; 1.
DR   PIRSF; PIRSF001458; MerB; 1.
DR   PRINTS; PR01699; ORGNOHGLYASE.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   3: Inferred from homology;
KW   Lyase; Mercuric resistance; Mercury.
FT   CHAIN           1..216
FT                   /note="Alkylmercury lyase"
FT                   /id="PRO_0000220362"
SQ   SEQUENCE   216 AA;  23595 MW;  06339129C3697C56 CRC64;
     MKNISEFSAQ LNQTFDQGEA VSMEWLFRPL LKMLAEGDPV PVEDIAAETG KPVEEVKQVL
     QTLPSVELDE QGRVVGYGLT LVPTPHRFEV DGKQLYAWCA LDTLMFPALI GRTVHIASPC
     HGTGKSVRLT VEPDRVVSVE PSTAVVSIVT PDEMASVRSA FCNNVHFFSS PSAAQDWLNQ
     HPESSVLPVE DAFELGRHLG ARYEESGPTN GSCCNI