ID   MERR_ACIFR              Reviewed;         122 AA.
AC   P22896;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Mercuric resistance operon regulatory protein;
DE   Flags: Fragment;
GN   Name=merR;
OS   Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans).
OG   Plasmid pTF-FC2.
OC   Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales;
OC   Acidithiobacillaceae; Acidithiobacillus.
OX   NCBI_TaxID=920;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1400173; DOI=10.1128/jb.174.19.6230-6237.1992;
RA   Rohrer J., Rawlings D.E.;
RT   "Sequence analysis and characterization of the mobilization region of a
RT   broad-host-range plasmid, pTF-FC2, isolated from Thiobacillus
RT   ferrooxidans.";
RL   J. Bacteriol. 174:6230-6237(1992).
CC   -!- FUNCTION: Mediates the mercuric-dependent induction of mercury
CC       resistance operon. In the absence of mercury MerR represses
CC       transcription by binding tightly to the mer operator region; when
CC       mercury is present the dimeric complex binds a single ion and becomes a
CC       potent transcriptional activator, while remaining bound to the mer
CC       site.
CC   -!- CAUTION: It is not sure if the metal-binding site is on position 81 or
CC       82. {ECO:0000305}.
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DR   EMBL; M57717; AAA27396.1; -; Genomic_DNA.
DR   PIR; I43256; I43256.
DR   AlphaFoldDB; P22896; -.
DR   SMR; P22896; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0046689; P:response to mercury ion; IEA:UniProtKB-KW.
DR   InterPro; IPR009061; DNA-bd_dom_put_sf.
DR   InterPro; IPR000551; MerR-type_HTH_dom.
DR   Pfam; PF13411; MerR_1; 1.
DR   PRINTS; PR00040; HTHMERR.
DR   SMART; SM00422; HTH_MERR; 1.
DR   SUPFAM; SSF46955; SSF46955; 1.
DR   PROSITE; PS00552; HTH_MERR_1; 1.
DR   PROSITE; PS50937; HTH_MERR_2; 1.
PE   4: Predicted;
KW   Activator; DNA-binding; Mercuric resistance; Mercury; Metal-binding;
KW   Plasmid; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..>122
FT                   /note="Mercuric resistance operon regulatory protein"
FT                   /id="PRO_0000098143"
FT   DOMAIN          5..74
FT                   /note="HTH merR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT   DNA_BIND        8..27
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT   BINDING         81
FT                   /ligand="Hg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:16793"
FT                   /evidence="ECO:0000250"
FT   BINDING         117
FT                   /ligand="Hg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:16793"
FT                   /evidence="ECO:0000250"
FT   NON_TER         122
SQ   SEQUENCE   122 AA;  13618 MW;  D067FD046497CBE3 CRC64;
     MEDTMHTIGK VATLAGVSTD TLRYYEKEHL IEPASRTAAG YRLYNDEAVR RIRFIKTAQH
     CGFSLSDIQE LLTLKRTDSA CCEDVRSVAI EKRLRIARKL RVLQAMSAAL NDLIQSCEGG
     AA